Information on EC 2.7.4.26 - isopentenyl phosphate kinase

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The expected taxonomic range for this enzyme is: Archaea, Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.4.26
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RECOMMENDED NAME
GeneOntology No.
isopentenyl phosphate kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + isopentenyl phosphate = ADP + isopentenyl diphosphate
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mevalonate pathway II (archaea)
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mevalonate pathway III (archaea)
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mevalonate metabolism
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Terpenoid backbone biosynthesis
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Biosynthesis of secondary metabolites
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
ATP:isopentenyl phosphate phosphotransferase
The enzyme is involved in the mevalonate pathway in Archaea [1]. The activity has also been identified in the plant Mentha piperita (peppermint) [2]. It is strictly specific for ATP but can use other phosphate acceptors such as dimethylallyl phosphate, geranyl phosphate, or fosfomycin.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (2E,6E)-farnesyl phosphate
ADP + (2E,6E)-farnesyl diphosphate
show the reaction diagram
ATP + but-3-en-1-yl phosphate
ADP + but-3-en-1-yl diphosphate
show the reaction diagram
ATP + butyl phosphate
ADP + butyl diphosphate
show the reaction diagram
ATP + dimethyl allylalcohol
ADP + dimethylallyl phosphate
show the reaction diagram
very low activity
-
-
?
ATP + dimethylallyl phosphate
ADP + dimethylallyl diphosphate
show the reaction diagram
ATP + fosfomycin
ADP + ((2R,3S)-3-methyloxiran-2-yl)phosphonic phosphoric anhydride
show the reaction diagram
ATP + geranyl phosphate
ADP + geranyl diphosphate
show the reaction diagram
ATP + isopentenyl phosphate
ADP + isopentenyl diphosphate
show the reaction diagram
ATP + isopentenyl thiolophosphate
ADP + isopentenyl thiolodiphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + isopentenyl phosphate
ADP + isopentenyl diphosphate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
fosfomycin
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0031 - 0.013
ADP
0.006 - 0.559
ATP
0.0297 - 0.103
but-3-en-1-yl phosphate
0.0263 - 0.173
butyl phosphate
0.0432 - 0.174
dimethylallyl phosphate
0.11 - 0.15
fosfomycin
0.74 - 4.7
geranyl phosphate
0.0027 - 0.0076
isopentenyl diphosphate
0.0028 - 0.256
isopentenyl phosphate
0.0145 - 0.0237
isopentenyl thiolophosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16.3
ATP
Haloferax volcanii
D4GWT7
pH 7.5, 30C
8.07 - 20.5
but-3-en-1-yl phosphate
9.9 - 31.8
butyl phosphate
11.3 - 34
dimethylallyl phosphate
0.03 - 0.04
fosfomycin
0.0142 - 0.047
geranyl phosphate
2.75 - 4.5
isopentenyl diphosphate
0.04 - 330
isopentenyl phosphate
2.84 - 8.22
isopentenyl thiolophosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
340 - 1200
ADP
13
1300 - 2200
ATP
4
7.8 - 69
but-3-en-1-yl phosphate
19570
5.7 - 120
butyl phosphate
15671
6.5 - 790
dimethylallyl phosphate
11615
0.0025 - 0.0026
fosfomycin
1153
0.01 - 0.19
geranyl phosphate
9852
1 - 2800
isopentenyl phosphate
4149
200 - 350
isopentenyl thiolophosphate
19569
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.4
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less than 50% of maximal activity above and below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55 - 75
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.06
calculated from sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
X-ray structure determination
X-ray structure determination
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
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stable up to
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli BL21
expression of an N-terminally His-tagged enzyme form in Haloferax volcanii H1209
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F83A/I86A/I156A
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the mutant shows a 27% rate of conversion of (2E,6E)-farnesyl phosphate to (2E,6E)-farnesyl diphosphate. The reaction is not catalyzed by the wild-type enzyme
H60Q
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His60 plays a key role in binding and catalysis, the mutant enzyme shows increased values for Km and decreased vaules for kcat and kcat/Km
F83A/I86A/I156A
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the mutant shows a 27% rate of conversion of (2E,6E)-farnesyl phosphate to (2E,6E)-farnesyl diphosphate. The reaction is not catalyzed by the wild-type enzyme
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H60Q
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His60 plays a key role in binding and catalysis, the mutant enzyme shows increased values for Km and decreased vaules for kcat and kcat/Km
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