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Literature summary for 2.7.4.1 extracted from
- Ellagic acid derivatives from Terminalia chebula Retz. increase the susceptibility of Pseudomonas aeruginosa to stress by inhibiting polyphosphate kinase (2015), J. Appl. Microbiol., 118, 817-825.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | enzyme PPK1 is an attractive therapeutic target to control infections caused by multiple drug resistant Pseudomonas aeruginosa | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | product inhibition | Pseudomonas aeruginosa |  |
| ellagic acid | ellagic acid derivatives from Terminalia chebula increase the susceptibility of Pseudomonas aeruginosa to stress by inhibiting polyphosphate kinase, oveview. Ellagic acid derivatives completely inhibit PPK1 activity at 0.5 mg/ml. Ellagic acid derivatives-treated Pseudomonas aeruginosa cells show marked reduction in polyphosphate granules in cytosol. Ellagic acid derivatives from Terminalia chebula inhibit PPK1 expression and its activity and increase the sensitivity of Pseudomonas aeruginosa to desiccation and oxidative stress while reducing tolerance to piperacillin | Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Pseudomonas aeruginosa | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | Pseudomonas aeruginosa | - |
ADP + (phosphate)n+1 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9S646 | gene ppk1, strains MPAO1 and ppk1 mutant PW9825, and clinical strains (P1-P11) from burn and sepsis patients | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | - |
Pseudomonas aeruginosa | ADP + (phosphate)n+1 | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| polyphosphate kinase 1 | - |
Pseudomonas aeruginosa |
| PPK1 | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Pseudomonas aeruginosa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Pseudomonas aeruginosa | ellagic acid derivatives reduce gene ppk1 expression by 93% 0.5 mg/ml. Ellagic acid derivatives-treated Pseudomonas aeruginosa cells show marked reduction in polyphosphate granules in cytosol | down |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | Pseudomonas aeruginosa strains wild-type MPAO1 and enzyme-deficient PW9825 survival in oxidative stress, overview | Pseudomonas aeruginosa |
| physiological function | polyphosphate kinase 1 plays an important role in virulence, antibiotic resistance and survival under stress conditions | Pseudomonas aeruginosa |
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