General Stability | Organism |
---|---|
ammonium chloride or neomycin B stabilizes, E. coli JR39 enzyme | Escherichia coli |
dithiothreitol stabilizes and restores activity of denatured enzyme | Staphylococcus aureus |
dithiothreitol stabilizes and restores activity of denatured enzyme | Escherichia coli |
dithiothreitol stabilizes and restores activity of denatured enzyme | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Gentamicin 1a | with neomycin as substrate, no inhibition in E. coli strain JR66/W677 or Pseudomonas aeruginosa strain Ps49 | Escherichia coli | |
Gentamicin 1a | with neomycin as substrate, no inhibition in E. coli strain JR66/W677 or Pseudomonas aeruginosa strain Ps49 | Pseudomonas aeruginosa | |
kanamycin | substrate inhibition at 2 mM | Staphylococcus aureus | |
tobramycin | - |
Escherichia coli | |
tobramycin | - |
Pseudomonas aeruginosa | |
tobramycin | - |
Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
kanamycin | pH 7.5, 35°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation at 1 mM, can replace Mg2+ | Pseudomonas aeruginosa | |
Mg2+ | - |
Pseudomonas aeruginosa | |
Mg2+ | required for activity | Escherichia coli | |
Mn2+ | activation at 1 mM, can replace Mg2+ | Pseudomonas aeruginosa | |
Zn2+ | activation at 1 mM, can replace Mg2+ | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strains JR35/W677, JR39/W677, JR66/W677, neomycin phosphotransferase II, ML1629, neomycin phosphotransferase I | - |
Pseudomonas aeruginosa | - |
neomycin phosphotransferase II | - |
Pseudomonas aeruginosa Ps49 | - |
neomycin phosphotransferase II | - |
Staphylococcus aureus | - |
multiple drug-resistant strain B294 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + kanamycin | specific for ATP | Staphylococcus aureus | ADP + kanamycin 3'-phosphate | - |
? | |
ATP + kanamycin | specific for ATP | Escherichia coli | ADP + kanamycin 3'-phosphate | - |
? | |
ATP + kanamycin | specific for ATP | Pseudomonas aeruginosa | ADP + kanamycin 3'-phosphate | - |
? | |
ATP + kanamycin | specific for ATP | Pseudomonas aeruginosa Ps49 | ADP + kanamycin 3'-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 37 | assay at | Staphylococcus aureus |
35 | 37 | assay at | Escherichia coli |
35 | 37 | assay at | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
strain ML1629, 5 min, inactivation | Escherichia coli |
55 | - |
strain H-9, 20 min stable | Pseudomonas aeruginosa |
65 | - |
strain H-9, 5 min stable | Pseudomonas aeruginosa |
75 | - |
strain H-9, inactivation | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | broad, no marked optimum | Escherichia coli |
5.5 | 8 | broad, no marked optimum | Pseudomonas aeruginosa |
7 | 7.5 | - |
Staphylococcus aureus |
7.5 | - |
with kanamycin | Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | stable | Pseudomonas aeruginosa |