Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli DF1020 | Entamoeba histolytica |
Protein Variants | Comment | Organism |
---|---|---|
H415A | site-directed mutagenesis, reduced activity, increased Km for fructose 6-phosphate and decreased Km for diphosphate | Entamoeba histolytica |
H416A | site-directed mutagenesis, reduced activity, slightly increased Km for fructose 6-phosphate | Entamoeba histolytica |
K408A | site-directed mutagenesis, activity , decreased Km for diphosphate | Entamoeba histolytica |
R377A | site-directed mutagenesis, increased Km values for fructose 6-phosphate and diphosphate | Entamoeba histolytica |
R405A | site-directed mutagenesis, increased activity, kinetic properties similar to the wild-type | Entamoeba histolytica |
R423A | site-directed mutagenesis, decrease of kcat by 10000fold, 10fold increased Km for diphosphate, 126fold increased Km for fructose 6-phosphate | Entamoeba histolytica |
S392A | site-directed mutagenesis, activity and kinetic properties similar to the wild-type | Entamoeba histolytica |
Y420A | site-directed mutagenesis, reduced activity, increased Km values for fructose 6-phosphate and diphosphate | Entamoeba histolytica |
Y420F | site-directed mutagenesis, reduced activity, increased Km for fructose 6-phosphate, decreased Km for diphosphate | Entamoeba histolytica |
Y420H | site-directed mutagenesis, reduced activity, increased Km values for fructose 6-phosphate and diphosphate | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values for substrates of mutant enzymes | Entamoeba histolytica | |
0.04 | - |
diphosphate | wild-type, pH 7.2, 30°C | Entamoeba histolytica | |
0.085 | - |
D-fructose 6-phosphate | wild-type, pH 7.2, 30°C | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate | Arg423 and Tyr 420 are involved in substrate binding and catalytic mechanism | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PPi-dependent phosphofructokinase | - |
Entamoeba histolytica |
PPi-PFK | - |
Entamoeba histolytica |
pyrophosphate-dependent phosphofructokinase | - |
Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Entamoeba histolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat values of mutant enzymes | Entamoeba histolytica | |
330 | - |
diphosphate | wild-type, pH 7.2, 30°C | Entamoeba histolytica | |
330 | - |
D-fructose 6-phosphate | wild-type, pH 7.2, 30°C | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Entamoeba histolytica |