Information on EC 2.7.1.90 - diphosphate-fructose-6-phosphate 1-phosphotransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.7.1.90
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RECOMMENDED NAME
GeneOntology No.
diphosphate-fructose-6-phosphate 1-phosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
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Biosynthesis of secondary metabolites
-
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Fructose and mannose metabolism
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Glycolysis / Gluconeogenesis
-
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glycolysis IV (plant cytosol)
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Metabolic pathways
-
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Microbial metabolism in diverse environments
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Pentose phosphate pathway
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glycolysis
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SYSTEMATIC NAME
IUBMB Comments
diphosphate:D-fructose-6-phosphate 1-phosphotransferase
The enzyme catalyses a similar reaction to EC 2.7.1.11, 6-phosphofructokinase, but utilizes diphosphate instead of ATP as the the phosphate donor. It has been described in higher plants, primitive eukaryotes, bacteria, and archaea.
CAS REGISTRY NUMBER
COMMENTARY hide
55326-40-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
strain B-PG9
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Manually annotated by BRENDA team
strain B-PG9
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
enzyme is completely replaced by an ATP-dependent phosphofructokinase, when cells grow on only one C1-carbon source, e.g. methanol
Uniprot
Manually annotated by BRENDA team
Q9SYP2 i.e. subunit alpha1, Q9C9K3 i.e. subunit alpha2, Q8W4M5, i.e. subunit beta1, F4JGR5 i.e. subunit beta2
Q9SYP2 and Q9C9K3 and Q8W4M5 and F4JGR5
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
var. botrytis
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-
Manually annotated by BRENDA team
not induced by 0.1 M NaCl
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-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
strain Rt46 B.1
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Manually annotated by BRENDA team
strain Rt46 B.1
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Manually annotated by BRENDA team
DKB-strain
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Manually annotated by BRENDA team
strain H200
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Manually annotated by BRENDA team
F2YQ40, i.e. alpha-subunit, F2YQ39, i.e. beta-subunit
F2YQ40 and F2YQ39
UniProt
Manually annotated by BRENDA team
strain Portland-1, ATCC 30888
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Manually annotated by BRENDA team
cv. Mandarin
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain L1
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-
Manually annotated by BRENDA team
strain L1
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-
Manually annotated by BRENDA team
Musa cavendishii
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-
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Manually annotated by BRENDA team
KVL-strain
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Manually annotated by BRENDA team
KVL-strain
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Manually annotated by BRENDA team
B G-6-strain
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Manually annotated by BRENDA team
B G-6-strain
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Manually annotated by BRENDA team
no activity in Acanthamoeba castellani
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Manually annotated by BRENDA team
no activity in Acanthamoeba castellani NEFF
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Manually annotated by BRENDA team
no activity in Mycoplasma gallisepticum S6
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-
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Manually annotated by BRENDA team
no activity in Rhodopseudomonas capsulata
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-
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Manually annotated by BRENDA team
no activity in Rhodopseudomonas capsulata Kb1
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Manually annotated by BRENDA team
no activity in Rhodopseudomonas palustris
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Manually annotated by BRENDA team
no activity in Rhodopseudomonas palustris strain 1a1
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Manually annotated by BRENDA team
no activity in Rhodopseudomonas sphaeroides
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Manually annotated by BRENDA team
no activity in Rhodopseudomonas viridis
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Manually annotated by BRENDA team
no activity in Spiroplasma floricola
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Manually annotated by BRENDA team
no activity in Spiroplasma floricola 23-6
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
2 enzyme forms
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-
Manually annotated by BRENDA team
cv. Top Crop
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain S1
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Manually annotated by BRENDA team
strain S1
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Manually annotated by BRENDA team
var. Hale
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Saccharum spp.
hybrid variety NCo310
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Manually annotated by BRENDA team
cv. Super First
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
PFP is important in carbohydrate metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(polyphosphate)n + D-fructose 6-phosphate
(polyphosphate)n-1 + D-fructose 1,6-bisphosphate
show the reaction diagram
arsenate + D-fructose 1,6-bisphosphate
?
show the reaction diagram
diphosphate + 2,5-anhydro-D-mannitol 6-phosphate
phosphate + 2,5-anhydro-D-mannitol 1,6-bisphosphate
show the reaction diagram
diphosphate + D-fructose 6-phosphate
phosphate + D-fructose 1,6-bisphosphate
show the reaction diagram
diphosphate + D-fructose 6-phosphate
phosphate + D-fructose 1,6-diphosphate
show the reaction diagram
diphosphate + D-ribulose 5-phosphate
?
show the reaction diagram
-
1.4% activity compared to D-fructose 6-phosphate
-
-
?
diphosphate + D-ribulose 5-phosphate
phosphate + ?
show the reaction diagram
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-
-
-
?
diphosphate + D-sedoheptulose 6-phosphate
phosphate + D-sedoheptulose 1,6-bisposphate
show the reaction diagram
diphosphate + D-sedoheptulose 7-phosphate
phosphate + D-sedoheptulose 1,7-bisphosphate
show the reaction diagram
diphosphate + sedoheptulose 7-phosphate
phosphate + ?
show the reaction diagram
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-
-
-
?
phosphate + D-fructose 1,6-bisphosphate
diphosphate + D-fructose 6-phosphate
show the reaction diagram
phosphate + D-fructose 1,6-diphosphate
diphosphate + D-fructose 6-phosphate
show the reaction diagram
phosphate + D-glucose 1,6-bisphosphate
?
show the reaction diagram
triphosphate + D-fructose 6-phosphate
diphosphate + D-fructose 1,6-bisphosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
diphosphate + D-fructose 6-phosphate
phosphate + D-fructose 1,6-bisphosphate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
the enzyme acts ATP-independent
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activates 120% at 0.1 mM
Divalent cations
Fe2+
-
activates 120% at 0.1 mM
Na+
-
activates 135% at 10 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,n-Alkanediol biphosphates
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2,3-O-isopropylidene-alpha-L-sorbofuranose-1-phosphate
2,5-anhydro-D-glucitol-6-phosphate
2,5-Anhydro-D-mannitol 1,6-bisphosphate
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D-fructose 1,6-bisphosphate-analog, kinetics
3-phospho-D-glycerate
3-phosphoglycerate
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3-[N-(2-phenylthioethyl)-N-methylamino]-1-hydroxypropylidene-1,1-bisphosphonate
i.e. CGP 48048, bisphosphonate, synthetic diphosphate analogue; inhibition of growth
alendronate
bisphosphonate, synthetic diphosphate analogue; inhibition of growth
bicarbonate
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-
citrate
clodronate
bisphosphonate, synthetic diphosphate analogue; inhibition of initial growth after inoculation
D-arabinose-5-phosphate
D-fructose 1,6-bisphosphate
D-fructose 2,6-bisphosphate
D-fructose 6-phosphate
D-glucitol-6-phosphate
D-glucose 6-phosphate
D-tagatose 6-phosphate
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kinetics, dead-end substrate
D-tagatose-6-phosphate
diethylene glycol-monophosphate
diphosphate
etidronate
bisphosphonate, synthetic diphosphate analogue; inhibition of growth
Fe3+
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84% inhibition at 0.1 mM
Imidodiphosphate
Increased ionic strength
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e.g. 30 mM KCl, NH4Cl, 10 mM Na2SO4, (NH4)2SO4 or 5 mM sodium phosphate
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KCl
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complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction
L-sorbose-6-phosphate
magnesium diphosphate
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reverse reaction, product inhibition, kinetics
malate
Methylenediphosphonate
nucleotides
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-
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pamidronate
bisphosphonate, synthetic diphosphate analogue; inhibition of growth
phosphate
phosphoenolpyruvate
Phosphoglycolate
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risedronate
bisphosphonate, synthetic diphosphate analogue; inhibition of growth
Se4+
-
75% inhibition at 0.1 mM
SO42-
sulfate
-
weak
Tetrapolyphosphate
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-
thiosulfate
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-
tungstate
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-
zoledronate
bisphosphonate, synthetic diphosphate analogue; inhibition of initial growth after inoculation
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine
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slight activation
Aminoimidazolecarboxamide riboside
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about 25% as efficient as AMP
citrate
CMP
-
slight activation
D-fructose 1,6-diphosphate
Musa cavendishii
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up to 24fold enhancement of glycolytic enzyme activity
D-fructose 2,6-bisphosphate
D-glucose
D-Glucose 1,6-bisphosphate
-
-
D-glucose 1-phosphate
dAMP
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activation, about 25% as efficient as AMP
GMP
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activation, about 35% as efficient as AMP
IMP
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activation, about 35% as efficient as AMP
MgADP-
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activation, about 15% as efficient as AMP
MgATP2-
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activation, about 15% as efficient as AMP
NaCl
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100 mM, increase in activity to 150% of control, inhibitory above 150 mM
NAD+
-
slight activation
phosphate
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slight activation
Polyethylene glycol
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stimulation, synergistic with AMP
UMP
-
slight activation
XMP
-
slight activation
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.92 - 0.97
2,5-anhydro-D-mannitol-6-phosphate
3.6
arsenate
0.009 - 2.9
D-fructose 1,6-bisphosphate
0.025 - 0.082
D-fructose 1,6-diphosphate
0.01 - 4600
D-fructose 6-phosphate
0.27
D-fructose1,6-bisphosphate
-
pH 8.0, 37°C
1.01
D-sedoheptulose 6-phosphate
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in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30°C
0.21 - 0.51
D-sedoheptulose 7-phosphate
0.005 - 0.58
diphosphate
0.023
fructose 1,6-bisphosphate
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reverse reaction, pH 7.8, 25°C
0.008 - 1.9
Mg2+
0.129 - 8.69
phosphate
0.027 - 0.49
sedoheptulose 7-phosphate
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06 - 330
D-fructose 6-phosphate
83 - 330
diphosphate
83
phosphate
Giardia intestinalis
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both reaction directions; pH 7.4, 30°C; substrate is a Mg2+-chelated complexe of phosphate, not uncomplexed ligand
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.22
1,6-hexanediol-monophosphate
-
forward reaction, pH 8.0, 25°C
2.37 - 6.7
2,3-O-isopropylidene-alpha-L-sorbofuranose-1-phosphate
0.32 - 1.52
2,5-anhydro-D-glucitol-6-phosphate
0.25
3-phosphoglycerate
-
pH 7.5
0.05
3-[N-(2-phenylthioethyl)-N-methylamino]-1-hydroxypropylidene-1,1-bisphosphonate
pH 7.0, 25°C
0.4
alendronate
pH 7.0, 25°C
20.2 - 56.9
Cl-
85
clodronate
pH 7.0, 25°C
3.62
D-arabinose-5-phosphate
-
forward reaction, pH 8.0, 25°C
0.013 - 0.086
D-fructose 1,6-bisphosphate
3.24 - 7.36
D-fructose 2,6-bisphosphate
0.113 - 0.62
D-fructose 6-phosphate
2.16 - 5.07
D-glucitol-6-phosphate
2.41 - 2.56
D-tagatose-6-phosphate
1.49 - 10.87
diethylene glycol-monophosphate
0.0025 - 1.3
diphosphate
0.56
etidronate
pH 7.0, 25°C
2.05 - 2.39
L-sorbose-6-phosphate
0.39 - 10.3
phosphate
0.3
risedronate
pH 7.0, 25°C
0.05
zoledronate
pH 7.0, 25°C
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY <