Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis of isozymes PanK3 and PanK1beta, overexpression of wild-type isozymes PanK3 and PanK1beta and chimeric mutants thereof in HEK293T cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeric mutant enzymes PanK1beta-3-1beta and PanK3-1beta-3 by combination of isozymes PanK3 and PanK1beta, mutant show different sensitivity to feedback inhibitors compared to the wild-type isozymes, overview | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | feedback inhibition, regulatory function, isozyme PanK1beta and isozyme PanK3, strong inhibition of mutant chimera PanK1beta-3-1beta, slight inhibition of mutant chimera PanK3-1beta-3 | Mus musculus | |
coenzyme A | feedback inhibition, regulatory function, isozyme PanK1beta, slight inhibition of isozyme PanK3 | Mus musculus | |
malonyl-CoA | feedback inhibition, regulatory function, isozyme PanK3, slight inhibition of isozyme PanK1beta | Mus musculus | |
additional information | structural determinants for feedback inhibition | Mus musculus | |
palmitoyl-CoA | feedback inhibition, regulatory function, isozyme PanK3, mutant chimera PanK1beta-3-1beta, slight inhibition of mutant chimera PanK3-1beta-3 | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0057 | - |
(R)-pantothenate | recombinant isozyme PanK1beta, pH 7.0, 37°C | Mus musculus | |
0.0095 | - |
(R)-pantothenate | recombinant isozyme PanK3, pH 7.0, 37°C | Mus musculus | |
0.087 | - |
ATP | recombinant isozyme PanK1beta, pH 7.0, 37°C | Mus musculus | |
0.112 | - |
ATP | recombinant isozyme PanK3, pH 7.0, 37°C | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41100 | - |
2 * 41100, isozyme Pank3, sequence calculation and gel filtration, 2 * 41600, isozyme Pank1beta, sequence calculation and gel filtration | Mus musculus |
41600 | - |
2 * 41100, isozyme Pank3, sequence calculation and gel filtration, 2 * 41600, isozyme Pank1beta, sequence calculation and gel filtration | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | Mus musculus | first step in coenzyme A biosynthesis, regulatory function | ADP + (R)-4'-phosphopantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
male C57BL6/J mice, isozyme PanK3 and PanK1beta | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | - |
Mus musculus | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | first step in coenzyme A biosynthesis, regulatory function | Mus musculus | ADP + (R)-4'-phosphopantothenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41100, isozyme Pank3, sequence calculation and gel filtration, 2 * 41600, isozyme Pank1beta, sequence calculation and gel filtration | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
PanK | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |