Crystallization (Comment) | Organism |
---|---|
apo histidinol-phosphate aminotransferase the internal PLP aldimine adduct and a pyridoxamine 5'-phosphate enzyme complex at resolutions of 2.2 A, 2.1 A and 1.8 A, respectively. The hydrogen bond between the side chain of residue Y21 and the phosphate group of histidinol phosphate is important for recognition of the natural substrate and discrimination against other potential amino donors. Residue N99 does not contribute to the specific recognition of the amino-acid donor. Residues Y123 and Y257 interact with the substrate through van der Waals-interactions | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
N99D | 6fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate | Corynebacterium glutamicum |
Y123F | 4fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate | Corynebacterium glutamicum |
Y21F | 10fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate | Corynebacterium glutamicum |
Y257F | 5fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.89 | - |
L-histidinol phosphate | wild-type | Corynebacterium glutamicum | |
1.3 | - |
L-histidinol phosphate | mutant Y123F | Corynebacterium glutamicum | |
2.2 | - |
L-histidinol phosphate | mutant Y257F | Corynebacterium glutamicum | |
6.7 | - |
L-histidinol phosphate | mutant Y21F | Corynebacterium glutamicum | |
9.1 | - |
L-histidinol phosphate | mutant N99D | Corynebacterium glutamicum | |
22.2 | - |
L-phenylalanine | mutant Y257F | Corynebacterium glutamicum | |
25.5 | - |
L-phenylalanine | mutant Y21F | Corynebacterium glutamicum | |
27.5 | - |
L-phenylalanine | mutant Y123F | Corynebacterium glutamicum | |
32.8 | - |
L-leucine | mutant Y257F | Corynebacterium glutamicum | |
53.4 | - |
L-leucine | mutant Y123F | Corynebacterium glutamicum | |
55.8 | - |
L-leucine | mutant N99D | Corynebacterium glutamicum | |
71.4 | - |
L-phenylalanine | mutant N99D | Corynebacterium glutamicum | |
80.8 | - |
L-leucine | mutant Y21F | Corynebacterium glutamicum | |
94.9 | - |
L-leucine | wild-type | Corynebacterium glutamicum | |
106 | - |
L-phenylalanine | wild-type | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q9KJU4 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
substrates 4-methyl-2-oxopentanoate, L-alanine | Corynebacterium glutamicum |
0.2 | - |
substrates L-glutarate, 2-oxobutanoate | Corynebacterium glutamicum |
0.3 | - |
substrates 3-indole-2-oxopropanoate, L-glutamate | Corynebacterium glutamicum |
0.4 | - |
substrates 4-methyl-2-oxopentanoate, L-asparagine | Corynebacterium glutamicum |
0.5 | - |
substrates 3-(4-hydroxyphenyl)-2-oxopropanoate, L-glutamate | Corynebacterium glutamicum |
1.4 | - |
substrates 4-methyl-2-oxopentanoate, L-glutamate | Corynebacterium glutamicum |
1.5 | - |
substrates phenylpyruvate, L-glutamate | Corynebacterium glutamicum |
2.8 | - |
substrates L-histidinol phosphate, 2-oxoglutarate | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
Corynebacterium glutamicum | L-tyrosine + 2-oxoglutarate | - |
? | |
3-indole-2-oxopropanoate + L-glutamate | - |
Corynebacterium glutamicum | L-tryptophan + 2-oxoglutarate | - |
? | |
4-methyl-2-oxopentanoate + L-alanine | - |
Corynebacterium glutamicum | L-leucine + pyruvate | - |
? | |
4-methyl-2-oxopentanoate + L-asparagine | - |
Corynebacterium glutamicum | L-leucine + 2-oxosuccinamic acid | - |
? | |
4-methyl-2-oxopentanoate + L-glutamate | - |
Corynebacterium glutamicum | L-leucine + 2-oxoglutarate | - |
? | |
L-glutarate + 2-oxobutanoate | - |
Corynebacterium glutamicum | 2-oxoglutarate + 2-aminobutanoate | - |
? | |
L-histidinol phosphate + 2-oxoglutarate | - |
Corynebacterium glutamicum | 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate | - |
? | |
additional information | no substrate: 3-methyl-2-oxopentanoate, 3-methyl-2-oxobutanoate | Corynebacterium glutamicum | ? | - |
? | |
phenylpyruvate + L-glutamate | - |
Corynebacterium glutamicum | L-phenylalanine + 2-oxoglutarate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
L-histidinol phosphate | mutant Y123F | Corynebacterium glutamicum | |
0.51 | - |
L-histidinol phosphate | mutant Y257F | Corynebacterium glutamicum | |
0.59 | - |
L-phenylalanine | mutant Y123F | Corynebacterium glutamicum | |
0.66 | - |
L-leucine | mutant Y123F | Corynebacterium glutamicum | |
0.68 | - |
L-phenylalanine | mutant Y257F | Corynebacterium glutamicum | |
0.69 | - |
L-leucine | mutant Y257F | Corynebacterium glutamicum | |
0.87 | - |
L-phenylalanine | mutant Y21F | Corynebacterium glutamicum | |
1.18 | - |
L-histidinol phosphate | wild-type | Corynebacterium glutamicum | |
1.6 | - |
L-leucine | mutant N99D | Corynebacterium glutamicum | |
1.72 | - |
L-leucine | mutant Y21F | Corynebacterium glutamicum | |
1.86 | - |
L-histidinol phosphate | mutant N99D | Corynebacterium glutamicum | |
1.93 | - |
L-histidinol phosphate | mutant Y21F | Corynebacterium glutamicum | |
2.22 | - |
L-phenylalanine | mutant N99D | Corynebacterium glutamicum | |
3.27 | - |
L-leucine | wild-type | Corynebacterium glutamicum | |
4.1 | - |
L-phenylalanine | wild-type | Corynebacterium glutamicum |