General Stability | Organism |
---|---|
freeze-thawing inactivates | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-ethylthioadenosine | - |
Escherichia coli | |
additional information | inhibition by end products of reaction; no inhibition by phenylhydrazine, semicarbazide, sodium borohydride, NaCN, KCl, NH4Cl, MgCl2, CaCl2, NaNO3, Na2SO4, Na2HPO4 | Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
p-hydroxymercuribenzoate | 2-mercaptoethanol restores activity | Escherichia coli | |
spermidine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
S-adenosyl-3-methylthio-1-propylamine | pH 8.0, 37ºC, fluorometric assay | Escherichia coli | |
0.012 | - |
1,4-diaminobutane | pH 8.8, 37ºC, spectrophotometric assay | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70600 | - |
W strain, sedimentation equilibrium centrifugation | Escherichia coli |
73000 | - |
W strain | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Escherichia coli | - |
5'-methylthioadenosine + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
W strain | - |
Purification (Comment) | Organism |
---|---|
2000fold purified | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.83 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, partially purified enzyme, several weeks with less than 20% loss of activity | Escherichia coli |
0-4°C, purified enzyme, 10-20% loss of activity within 6 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
ir | |
S-adenosyl-(5')-3-methylthio-1-propylamine + spermidine | at a reduced rate | Escherichia coli | 5'-methylthioadenosine + spermine | the reaction rate is slower than with 1,4-diaminobutane | ? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane | - |
Escherichia coli | 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000-35000, SDS-PAGE | Escherichia coli |
dimer | 2 * 37000-38000, high speed sedimentation equilibrium centrifugation in 6 M guanidine-HCl and 0.1 M 2-mercaptoethanol | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
at least 30 min stable in 0.01 M Tris-HCl buffer, pH 8, 1 mM EDTA | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.4 | - |
65% rate of reaction at pH 8.6 and 93% rate of reaction at pH 10.6 | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.6 | 10.6 | about 65% of maximal activity at pH 8.6 and about 90% of maximal activity at pH 10.6 | Escherichia coli |