Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl 3-(methylthio)propylamine + spermidine | Thermococcus kodakarensis | N4-bis(aminopropyl)spermidine is the major polyamine in Thermococcus kodakarensis. The branched-chain polyamine synthesized by BpsA is important for cell growth at 93°C | 2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine | overall reaction | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JIZ3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Thermococcus kodakarensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = 2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine | overall reaction | Thermococcus kodakarensis | |
S-adenosyl 3-(methylsulfanyl)propylamine + N4-aminopropylspermidine = S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine | (1b) | Thermococcus kodakarensis | |
S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = S-methyl-5'-thioadenosine + N4-aminopropylspermidine | (1a) | Thermococcus kodakarensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
pH 9.0, 70°C, production of N4-aminopropylspermine | Thermococcus kodakarensis |
0.34 | - |
pH 9.0, 70°C, production of N4-bis(aminopropyl)spermidine | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl 3-(methylthio)propylamine + spermidine | - |
Thermococcus kodakarensis | 2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine | overall reaction. N4-bis(aminopropyl)spermidine is produced from spermidine by the sequential reactions catalyzed by BpsA via N4-aminopropylspermidine | ? | |
2 S-adenosyl 3-(methylthio)propylamine + spermidine | N4-bis(aminopropyl)spermidine is the major polyamine in Thermococcus kodakarensis. The branched-chain polyamine synthesized by BpsA is important for cell growth at 93°C | Thermococcus kodakarensis | 2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine | overall reaction | ? | |
S-adenosyl 3-(methylthio)propylamine + N4-aminopropylspermine | - |
Thermococcus kodakarensis | S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine | - |
? | |
S-adenosyl 3-(methylthio)propylamine + spermidine | - |
Thermococcus kodakarensis | S-methyl-5'-thioadenosine + N4-aminopropylspermidine | - |
? | |
S-adenosyl 3-(methylthio)propylamine + spermine | - |
Thermococcus kodakarensis | S-methyl-5'-thioadenosine + N4-aminopropylspermine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BpsA | - |
Thermococcus kodakarensis |
TK1691 | locus name | Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Thermococcus kodakarensis |
General Information | Comment | Organism |
---|---|---|
malfunction | at 85°C the bspA disruptant strain shows a slightly extended lag phase compared to that of the wild-type strain. At 93°C there is no cell growth of the disruptant strain. The bpsA gene is required for growth at the higher temperature. The growth defect of the disruptant strain at 93°C is partially restored by the addition of 1 mM N4-bis(aminopropyl)spermidine to the medium. N4-bis(Aminopropyl)spermidine is required for cell growth of Thermococcus kodakarensis in a higher-temperature environment | Thermococcus kodakarensis |
physiological function | N4-bis(aminopropyl)spermidine is the major polyamine in Thermococcus kodakarensis. The branched-chain polyamine synthesized by BpsA is important for cell growth at 93°C | Thermococcus kodakarensis |