Information on EC 2.5.1.128 - N4-bis(aminopropyl)spermidine synthase

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The expected taxonomic range for this enzyme is: Thermococcus kodakarensis

EC NUMBER
COMMENTARY hide
2.5.1.128
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RECOMMENDED NAME
GeneOntology No.
N4-bis(aminopropyl)spermidine synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 S-adenosyl 3-(methylthio)propylamine + spermidine = 2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + N4-aminopropylspermidine = S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + spermidine = S-methyl-5'-thioadenosine + N4-aminopropylspermidine
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
polyamine pathway
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl 3-(methylthio)propylamine:spermidine 3-aminopropyltransferase (N4-bis(aminopropyl)spermidine synthesizing)
The enzyme, characterized from the thermophilic archaeon Thermococcus kodakarensis, synthesizes the branched-chain polyamine N4-bis(aminopropyl)spermidine, which is required for cell growth at high-temperature. When spermine is used as substrate, the enzyme forms N4-aminopropylspermine.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
at 85°C the bspA disruptant strain shows a slightly extended lag phase compared to that of the wild-type strain. At 93°C there is no cell growth of the disruptant strain. The bpsA gene is required for growth at the higher temperature. The growth defect of the disruptant strain at 93°C is partially restored by the addition of 1 mM N4-bis(aminopropyl)spermidine to the medium. N4-bis(Aminopropyl)spermidine is required for cell growth of Thermococcus kodakarensis in a higher-temperature environment
physiological function
N4-bis(aminopropyl)spermidine is the major polyamine in Thermococcus kodakarensis. The branched-chain polyamine synthesized by BpsA is important for cell growth at 93°C
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl 3-(methylthio)propylamine + spermidine
2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + N4-aminopropylspermine
S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine
show the reaction diagram
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-
-
?
S-adenosyl 3-(methylthio)propylamine + spermidine
S-methyl-5'-thioadenosine + N4-aminopropylspermidine
show the reaction diagram
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-
-
?
S-adenosyl 3-(methylthio)propylamine + spermine
S-methyl-5'-thioadenosine + N4-aminopropylspermine
show the reaction diagram
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-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl 3-(methylthio)propylamine + spermidine
2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine
show the reaction diagram
Q5JIZ3
N4-bis(aminopropyl)spermidine is the major polyamine in Thermococcus kodakarensis. The branched-chain polyamine synthesized by BpsA is important for cell growth at 93°C
overall reaction
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?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.12
pH 9.0, 70°C, production of N4-aminopropylspermine
0.34
pH 9.0, 70°C, production of N4-bis(aminopropyl)spermidine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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