Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
in complex with natural substrates and inhibitors, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M NaF, 0.1 M bis-Tris propane pH 6.5 | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ibandronate | - |
Pseudomonas aeruginosa | |
zoledronate | - |
Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | contains Mg2+ | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34018 | - |
ESI-MS | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + isopentenyl diphosphate | Pseudomonas aeruginosa | - |
diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
isopentenyl diphosphate + dimethylallyl diphosphate | Pseudomonas aeruginosa | - |
diphosphate + geranyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HWY4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + isopentenyl diphosphate | - |
Pseudomonas aeruginosa | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
isopentenyl diphosphate + dimethylallyl diphosphate | - |
Pseudomonas aeruginosa | diphosphate + geranyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34018, calculated from amino acid sequence | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
farnesyl pyrophosphate synthase | - |
Pseudomonas aeruginosa |
FPPS | - |
Pseudomonas aeruginosa |
PA4043 | - |
Pseudomonas aeruginosa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0027 | - |
at pH 8.0 and 22°C | Pseudomonas aeruginosa | ibandronate | |
0.0152 | - |
at pH 8.0 and 22°C | Pseudomonas aeruginosa | zoledronate |