Cloned (Comment) | Organism |
---|---|
expression of wild-type and chimeric enzymes in enzyme-deficient Escherichia coli strain, complementation study | Homo sapiens |
expression of wild-type and chimeric enzymes in enzyme-deficient Escherichia coli strain, complementation study, overexpression of chimeric mutant DS1 | Plasmodium falciparum |
overexpression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes, altered substrate specificities | Homo sapiens |
additional information | construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes, altered substrate specificities | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
hypoxanthine | wild-type enzyme | Homo sapiens | |
0.0011 | - |
guanine | recombinant chimeric mutant DS1 | Homo sapiens | |
0.0011 | - |
guanine | recombinant chimeric mutant DS1 | Plasmodium falciparum | |
0.0014 | - |
hypoxanthine | recombinant chimeric mutant DS1 | Homo sapiens | |
0.0014 | - |
hypoxanthine | recombinant chimeric mutant DS1 | Plasmodium falciparum | |
0.005 | - |
guanine | wild-type enzyme | Homo sapiens | |
0.3 | - |
xanthine | above, purified recombinant chimeric enzyme DS1 | Homo sapiens | |
0.3 | - |
xanthine | above, purified recombinant chimeric enzyme DS1 | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes | - |
Plasmodium falciparum | - |
construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.032 | - |
purified recombinant chimeric enzyme DS1, substrate guanine | Homo sapiens |
0.032 | - |
purified recombinant chimeric enzyme DS1, substrate guanine | Plasmodium falciparum |
0.147 | - |
purified recombinant chimeric enzyme DS1, substrate xanthine | Homo sapiens |
0.147 | - |
purified recombinant chimeric enzyme DS1, substrate xanthine | Plasmodium falciparum |
0.226 | - |
purified recombinant chimeric enzyme DS1, substrate hypoxanthine | Homo sapiens |
0.226 | - |
purified recombinant chimeric enzyme DS1, substrate hypoxanthine | Plasmodium falciparum |
11.9 | - |
purified recombinant enzyme, substrate hypoxanthine | Homo sapiens |
25.8 | - |
purified recombinant enzyme, substrate guanine | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | GMP + diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | IMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | IMP + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | no activity | Homo sapiens | XMP + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | recombinant chimeric mutant DS1 | Homo sapiens | XMP + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | recombinant chimeric mutant DS1 | Plasmodium falciparum | XMP + diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
guanine | purified recombinant chimeric enzyme DS1 | Homo sapiens | |
0.013 | - |
guanine | purified recombinant chimeric enzyme DS1 | Plasmodium falciparum | |
0.062 | - |
xanthine | purified recombinant chimeric enzyme DS1 | Homo sapiens | |
0.062 | - |
xanthine | purified recombinant chimeric enzyme DS1 | Plasmodium falciparum | |
0.073 | - |
hypoxanthine | purified recombinant chimeric enzyme DS1 | Homo sapiens | |
0.073 | - |
hypoxanthine | purified recombinant chimeric enzyme DS1 | Plasmodium falciparum | |
5.7 | - |
hypoxanthine | wild-type enzyme | Homo sapiens | |
10.5 | - |
guanine | wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.4 | - |
assay at | Plasmodium falciparum |