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Literature summary for 2.4.1.B52 extracted from
- Bonds broken and formed during the mixed-linkage glucan xyloglucan endotransglucosylase reaction catalysed by Equisetum hetero-trans-beta-glucanase (2017), Biochem. J., 474, 1055-1070 .
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equisetum fluviatile | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | attack of the site of hetero-trans-beta-glucanase (HTG) on one of its donor substrates, mixed-linkage (1->3),(1->4)-beta-D-glucan (MLG, from Equisetum arvense or Hordeum vulgare) is investigated with radioactive oligosaccharides of xyloglucan as the acceptor substrate. Analysis of the structure of the mixed-linkage glucan:xyloglucan endotransglucosylase (MXE) reaction product formed with [3H]XXXGol as the acceptor substrate and barley MLG as the donor substrate via digestion of the product with lichenase, method, overview. MXE forms a (Glc2)-XXXGol disaccharide product, which further analysis reveals as GGXXXGol, where GG is cellobiose. The bond between the GG and the XXXGol moiety is also likely to be a beta-(1->4)-bond because it can be cleaved by cellobiohydrolase. When the tetradecasaccharide [3H]XXXGXXXGol is used as an acceptor substrate, the single radioactive product is GG[3H]XXXGXXXGol. MXE has attached MLG to the non-reducing terminal heptasaccharide subunit alone. Identification of the bond initially cleaved by MXE activity | Equisetum fluviatile | ? | - |
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Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mixed-linkage glucan:xyloglucan endotransglucosylase | - |
Equisetum fluviatile |
| MXE | - |
Equisetum fluviatile |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Equisetum fluviatile |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | mixed-linkage glucan:xyloglucan endotransglucosylase (MXE) is one of the three activities of the recently characterised hetero-trans-beta-glucanase (HTG), which among land plants is known only from Equisetum species. The enzyme MXE belongs to the GH16 family | Equisetum fluviatile |
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