Information on EC 2.4.1.B52 - mixed-linkage glucan endootransglucosylase

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The expected taxonomic range for this enzyme is: Equisetum

EC NUMBER
COMMENTARY hide
2.4.1.B52
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
mixed-linkage glucan endootransglucosylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
transfers a (1->3),(1->4) mixed-linkage glucan to a xyloglucan
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
mixed-linkage glucan:xyloglucan glucanotransferase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
no activity in Hordeum vulgare
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
mixed-linkage (1->3, 1->4)-beta-D-glucan + (Xyl)3-D-glucitol
?
show the reaction diagram
-
-
-
?
tamarind xyloglucan + (Xyl)3-D-Glc
?
show the reaction diagram
-
-
-
?
additional information
?
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0014
(Xyl)3-D-Glc
pH 5.5, 20°C
-
0.00052
(Xyl)3-D-glucitol
pH 5.5, 20°C
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0044
(Xyl)3-D-glucitol
Equisetum fluviatile
A0A0K2WA62
pH 5.5, 20°C
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.5
(Xyl)3-D-glucitol
Equisetum fluviatile
A0A0K2WA62
pH 5.5, 20°C
210493
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.1
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
highest in brown, over-wintered leaves compared to young and mature leaves
Manually annotated by BRENDA team
-
MXE and XET, EC 2.4.1.207, actions are both detectable in living Equisetum fluviatile shoots, the MXE:XET ratio increasing with age
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 31000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
homology modeling of structure suggests that key amino acid substitutions Trp-Pro, Gly-Ser and Arg-Leu are responsible for the evolution of HTG’s specificity from the xyloglucan-acting homo-transglycanases
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Pichia pastoris