Cloned (Comment) | Organism |
---|---|
His-tagged protein and His-tagged fusion proteins harboring a cellulose binding module expressed in Escherichia coli BL21 | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C | Acetivibrio thermocellus | |
0.53 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C | Acetivibrio thermocellus | |
0.59 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C | Acetivibrio thermocellus | |
0.62 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C | Acetivibrio thermocellus | |
0.76 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C | Acetivibrio thermocellus | |
0.78 | - |
cellobiose | synthesis direction, wild type protein, pH 7.2, 30°C | Acetivibrio thermocellus | |
1.08 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C | Acetivibrio thermocellus | |
1.29 | - |
celloheptaose | synthesis direction, wild type protein, pH 7.2, 30°C | Acetivibrio thermocellus | |
1.46 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C | Acetivibrio thermocellus | |
1.53 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C | Acetivibrio thermocellus | |
1.56 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C | Acetivibrio thermocellus | |
1.82 | - |
celloheptaose | degradation direction, wild type protein, pH 7.2, 30°C | Acetivibrio thermocellus | |
1.93 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.71 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C | Acetivibrio thermocellus | |
5.24 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C | Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
x * 115000, SDS-PAGE | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + alpha-D-glucose 1-phosphate | weak activities on celloheptaose and insoluble regenerated amorphous cellulose | Acetivibrio thermocellus | cellotriose + phosphate | - |
r | |
celloheptaose + alpha-D-glucose 1-phosphate | weak activities on celloheptaose and insoluble regenerated amorphous cellulose | Acetivibrio thermocellus | cellooctaose + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 115000, SDS-PAGE | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
cellodextrin phosphorylase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
wild type and fusion proteins | Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.08 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C | Acetivibrio thermocellus | |
1.37 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C | Acetivibrio thermocellus | |
1.83 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.07 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.08 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.16 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.25 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.55 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.67 | - |
cellobiose | synthesis direction, wild type protein, pH 7.2, 30°C | Acetivibrio thermocellus | |
2.7 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.98 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C | Acetivibrio thermocellus | |
3.11 | - |
celloheptaose | synthesis direction, wild type protein, pH 7.2, 30°C | Acetivibrio thermocellus | |
3.77 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C | Acetivibrio thermocellus | |
4.14 | - |
celloheptaose | degradation direction, wild type protein, pH 7.2, 30°C | Acetivibrio thermocellus | |
4.53 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
wild type and fusion proteins | Acetivibrio thermocellus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C | Acetivibrio thermocellus | |
0.56 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C | Acetivibrio thermocellus | |
0.9 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C | Acetivibrio thermocellus | |
1 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.04 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.27 | - |
celloheptaose | degradation direction, wild type protein, pH 7.2, 30°C | Acetivibrio thermocellus | |
2.36 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.41 | - |
celloheptaose | synthesis direction, wild type protein, pH 7.2, 30°C | Acetivibrio thermocellus | |
2.73 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C | Acetivibrio thermocellus | |
2.9 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C | Acetivibrio thermocellus | |
3.1 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C | Acetivibrio thermocellus | |
3.42 | - |
cellobiose | synthesis direction, wild type protein, pH 7.2, 30°C | Acetivibrio thermocellus | |
3.9 | - |
cellobiose | synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C | Acetivibrio thermocellus | |
6.08 | - |
celloheptaose | degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C | Acetivibrio thermocellus | |
7.76 | - |
celloheptaose | synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C | Acetivibrio thermocellus |