Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful as catalyst for quantitative synthesis of Gal-beta-1,4-Man-pNP, overview | Neisseria meningitidis |
synthesis | the enzyme is useful as catalyst for quantitative synthesis of the thiodisaccharide Gal-betaS-1,4-GlcNAcpNP as well as Gal-beta-1,4-Man-pNP, overview | Helicobacter pylori |
Cloned (Comment) | Organism |
---|---|
expression of full-length HP0826 in Escherichia coli | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Neisseria meningitidis | |
additional information | - |
additional information | kinetics | Helicobacter pylori | |
0.27 | - |
UDP-Gal | pH 7.2 | Helicobacter pylori | |
0.79 | - |
pNP-beta-GlcNAc | pH 7.2 | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Helicobacter pylori | HP0826 is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori | ? | - |
? | |
additional information | Neisseria meningitidis | LgtB is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Neisseria meningitidis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
160 | - |
purified recombinant enzyme | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | HP0826 is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori | Helicobacter pylori | ? | - |
? | |
additional information | LgtB is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori | Neisseria meningitidis | ? | - |
? | |
additional information | HP0826 exhibits a pronounced acceptor promiscuity with the most striking result being the successful transfer of Gal to the 4-SH of pNP-4-S-beta-GlcNAc | Helicobacter pylori | ? | - |
? | |
UDP-Gal + 4-nitrophenyl 2-acetamido-2-deoxy-4-thio-beta-D-glucopyranoside | HP0826 catalyzes the synthesis of the thiodisaccharide Gal-beta-S-1,4-GlcNAcpNP as well as Gal-beta-1,4-Man-pNP with total regio- and stereoselectivity, overview | Helicobacter pylori | UDP + Gal-beta-S-1,4-GlcNAcpNP | - |
? | |
UDP-Gal + 4-nitrophenyl beta-D-mannopyranoside | HP0826 catalyzes the synthesis of the thiodisaccharide Gal-beta-S-1,4-GlcNAcpNP as well as Gal-beta-1,4-Man-pNP with total regio- and stereoselectivity, overview | Helicobacter pylori | UDP + Gal-beta-1,4-Man-pNP | - |
? | |
UDP-Gal + 4-nitrophenyl beta-D-mannopyranoside | LgtB catalyzes the synthesis of Gal-beta-1,4-Man-pNP, overview | Neisseria meningitidis | UDP + Gal-beta-1,4-Man-pNP | - |
? | |
UDP-Gal + 4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
Helicobacter pylori | UDP + ? | - |
? | |
UDP-galactose + p-nitrophenyl-beta-D-N-acetylglucosaminide | - |
Helicobacter pylori | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-1,4-galactosyltransferase | - |
Neisseria meningitidis |
beta-1,4-galactosyltransferase | - |
Helicobacter pylori |
HP0826 | - |
Helicobacter pylori |
LgtB | - |
Neisseria meningitidis |
More | HP0826 belongs to the carbohydrate active enzyme family GT25 of inverting glycosyltransferases | Helicobacter pylori |
More | LgtB belongs to the carbohydrate active enzyme family GT25 of inverting glycosyltransferases | Neisseria meningitidis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Helicobacter pylori |