Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by divalent cations, 0.1-10 mM, EDTA, EGTA, no substrate inhibition | Pisum sativum | |
UDP | product inhibition, 0.25 mM, 50% inhibition | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside | pH 7.3, 30°C | Pisum sativum | |
0.8 | - |
UDP-glucose | pH 7.3, 30°C | Pisum sativum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by divalent cations, 0.1-10 mM | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pisum sativum | one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
var. Alaska | - |
Purification (Comment) | Organism |
---|---|
30.7fold, separation of three distinct flavonol:glucosyltransferases | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | light-grown, one week old | Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pisum sativum |
Storage Stability | Organism |
---|---|
-15°C or room temperature, partially purified enzyme, 36 h, 80% loss of activity | Pisum sativum |
4°C, 2 weeks, stable | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides | Pisum sativum | ? | - |
? | |
additional information | no glucosylation of pelargonidin, naringenin, naringin and p-coumaric acid by the three flavonol:glucosyltransferases, not: ADP-glucose, GDP-glucose, kaempferol, kaempferol monoglucoside, quercetin-3-rutinoside | Pisum sativum | ? | - |
? | |
TDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside | 3fold lower rate than with UDP-glucose as glucosyl donor | Pisum sativum | TDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside | - |
? | |
UDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside | highly specific enzyme, specific for flavonol-3-O-diglucosides, 3fold higher rate than with TDP-glucose as glucosyl donor | Pisum sativum | UDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside | best substrate | Pisum sativum | UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside | - |
? | |
UDP-glucose + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside | 32% of the activity with kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside | Pisum sativum | UDP + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside | - |
? | |
UDP-glucose + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside | 14% of the activity with kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside | Pisum sativum | UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose:glucosyltransferase | - |
Pisum sativum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
in 0.2 M Tris-HCl buffer, two pH optima, equally active in 0.2 M Tris-HCl and 0.2 M glycine-NaOH buffer | Pisum sativum |
8.3 | - |
in 0.2 M glycine-NaOH buffer, two pH optima, equally active in 0.2 M Tris-HCl and 0.2 M glycine-NaOH buffer | Pisum sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor requirement | Pisum sativum |