We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments One of three specific glucosyltransferases in pea (Pisum sativum) thatsuccessively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside (see also EC 2.4.1.91 flavonol 3-O-glucosyltransferase, and EC 2.4.1.239 flavonol-3-O-glucoside glucosyltransferase).
The expected taxonomic range for this enzyme is: Pisum sativum
Synonyms
UDP-glucose:glucosyltransferase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-glucose:glucosyltransferase
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucosyl-(1->2)-beta-D-glucoside
UDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucosyl-(1->2)-beta-D-glucoside
one of three specific glucosyltransferases in pea, Pisum sativum, that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside, see also EC 2.4.1.91 flavonol 3-O-glucosyltransferase, and EC 2.4.1.239 flavonol-3-O-glucoside glucosyltransferase
-
UDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucosyl-(1->2)-beta-D-glucoside
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
transfer of glycosyl group
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-glucose:flavonol-3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside 2'''-O-beta-D-glucosyltransferase
One of three specific glucosyltransferases in pea (Pisum sativum) thatsuccessively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside (see also EC 2.4.1.91 flavonol 3-O-glucosyltransferase, and EC 2.4.1.239 flavonol-3-O-glucoside glucosyltransferase).
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
TDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
3fold lower rate than with UDP-glucose as glucosyl donor
-
-
?
UDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
highly specific enzyme, specific for flavonol-3-O-diglucosides, 3fold higher rate than with TDP-glucose as glucosyl donor
-
-
?
UDP-glucose + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP-glucose + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
32% of the activity with kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
-
?
UDP-glucose + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
additional information
?
-
UDP-glucose + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
-
-
-
?
UDP-glucose + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
best substrate
-
-
?
UDP-glucose + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
-
-
-
?
UDP-glucose + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
14% of the activity with kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
-
?
additional information
?
-
-
involved in the biosynthesis of flavonol triglucosides
-
-
?
additional information
?
-
-
one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides
-
-
?
additional information
?
-
-
glucosylation of kaempferol and quercetin in the 3-position to form the 3-O-triglucoside, reaction proceeds from the aglycone via the mono- and diglucoside intermediates, this reaction sequence is probably associated with three distinct enzyme activities
-
-
?
additional information
?
-
-
no glucosylation of pelargonidin, naringenin, naringin and p-coumaric acid by the three flavonol:glucosyltransferases, not: ADP-glucose, GDP-glucose, kaempferol, kaempferol monoglucoside, quercetin-3-rutinoside
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
?
-
additional information
?
-
-
involved in the biosynthesis of flavonol triglucosides
-
-
?
additional information
?
-
-
one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
no cofactor requirement
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
not activated by divalent cations, 0.1-10 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP
-
product inhibition, 0.25 mM, 50% inhibition
additional information
-
not inhibited by divalent cations, 0.1-10 mM, EDTA, EGTA, no substrate inhibition
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.008
kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
pH 7.3, 30°C
0.8
UDP-glucose
-
pH 7.3, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.6
-
in 0.2 M Tris-HCl buffer, two pH optima, equally active in 0.2 M Tris-HCl and 0.2 M glycine-NaOH buffer
8.3
-
in 0.2 M glycine-NaOH buffer, two pH optima, equally active in 0.2 M Tris-HCl and 0.2 M glycine-NaOH buffer
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
var. Alaska
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
young leaf tissues have much higher levels of glucosyltransferase activity than the petioles and internodes
brenda
-
light-grown, one week old
brenda
-
young, light-grown, much higher levels of glucosyltransferase activity in young leaf tissues than in petioles and internodes
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-15°C or room temperature, partially purified enzyme, 36 h, 80% loss of activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
30.7fold, separation of three distinct flavonol:glucosyltransferases
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Jourdan, P.S.; Mansell, R.L.
Isolation and partial characterization of three glucosyl transferases involved in the biosynthesis of flavonol triglucosides in Pisum sativum L
Arch. Biochem. Biophys.
213
434-443
1982
Pisum sativum
brenda
Shute, J.L.; Jourdan, P.S.; Mansell, R.L.
UDP-glucose:glucosyltransferase activity involved in the biosynthesis of flavonol triglucosides in Pisum sativum L. seedlings
Z. Naturforsch. C
34
738-741
1979
Pisum sativum
brenda
Select items on the left to see more content.
html completed