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Literature summary for 2.4.1.208 extracted from

  • Kiriukhin, M.Y.; Debabov, D.V.; Shinabarger, D.L.; Neuhaus, F.C.
    Biosynthesis of the glycolipid anchor in lipoteichoic acid of Staphylococcus aureus RN4220: role of YpfP, the diglucosyldiacylglycerol synthase (2001), J. Bacteriol., 183, 3506-3514.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.208

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Staphylococcus aureus
expression in Escherichia coli Bacillus subtilis

General Stability

General Stability Organism
activity of the recombinant enzyme does not change during 3 months of storage and upon multiple cycles of freezing and thawing Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 UDP-glucose
-
 Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol Staphylococcus aureus enzyme provides glycolipid for lipoteichoic acid assembly UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
 ?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol Staphylococcus aureus RN4220 enzyme provides glycolipid for lipoteichoic acid assembly UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Staphylococcus aureus
-
-
-
Staphylococcus aureus RN4220
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
-
 Staphylococcus aureus UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
 ?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
-
 Bacillus subtilis UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
 ?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol enzyme provides glycolipid for lipoteichoic acid assembly Staphylococcus aureus UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
 ?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
-
 Staphylococcus aureus RN4220 UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
 ?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol enzyme provides glycolipid for lipoteichoic acid assembly Staphylococcus aureus RN4220 UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Staphylococcus aureus