Information on EC 2.4.1.208 - diglucosyl diacylglycerol synthase (1,2-linking)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.208
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RECOMMENDED NAME
GeneOntology No.
diglucosyl diacylglycerol synthase (1,2-linking)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->2)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
alpha-diglucosyldiacylglycerol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol 2-glucosyltransferase
The enzyme from Acholeplasma laidlawii requires Mg2+.
CAS REGISTRY NUMBER
COMMENTARY hide
168680-19-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain A-EF22, simple, cell-wall-less prokaryotic parasite
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain RN4220
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Manually annotated by BRENDA team
strain RN4220
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1-2)-O-alpha-D-glucopyranosyl)sn-glycerol + UDP
show the reaction diagram
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
show the reaction diagram
UDP-glucose + 1,2-diheptadecanoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diheptadecanoyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
show the reaction diagram
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best substrate
73% of activity with monoglucosyldioleoylglycerol
?
UDP-glucose + 1,2-dioleoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-dioleoyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1-2)-O-alpha-D-glucopyranosyl)sn-glycerol + UDP
show the reaction diagram
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol
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1,2-diacyl-3-O-[6-O-acyl(alpha-D-glucopyranosyl)]-sn-glycerol
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1,2-diacyl-3-O-[alpha-D-glucopyranosyl-1,2-O-(6-O-acyl-alpha-D-glucopyranosyl)]-sn-glycerol
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ATP
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above 1.5 mM, stimulation below
phosphenolpyruvate
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above 1.5 mM
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sn-glycero-3-phosphate
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20 mM, complete inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-dioleoylphosphatidylethanolamine
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maximal activity at 4.5%
1,2-dioleoylphosphatidylglycerol
1,2-dioleoylphosphatidylserine
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33% of activation with 1,2-dioleoylphosphatidylglycerol
1,3-diacylglycerol
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1,3-dioleoylglycerol
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11.5 mol%, 5fold activation
ATP
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approx. 12fold stimulation at 0.75 mM, inhibition above 1.5 mM
cardiolipin
diphosphatidylglycerol
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from bovine, 50% of activation with 1,2-dioleoylphosphatidylglycerol
dsDNA
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0.0007 mg/ml, 7fold activation
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fructose-1,6-bisphosphate
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1 mM, approx. 18fold stimulation
Lipid
phosphate
phosphatidylglycerol
phosphatidylinositol
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strong activation in the presence of 1,2-dioleoylphosphatidylglycerol
phosphatidylinositol-4,5-bisphosphate
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strong activation in the presence of 1,2-dioleoylphosphatidylglycerol
phosphoenolpyruvate
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0.5 mM, 4fold stimulation, inhibition above 1.5 mM
sn-glycero-1-phosphate
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stimulates above 15 mM
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14 - 0.7
UDP-glucose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity of the recombinant enzyme does not change during 3 months of storage and upon multiple cycles of freezing and thawing
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
solubilization, SP-Sepharose, hydroxyapatite, Green A agarose
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme can be reconstituted in liposome bilayers by the dialyse techique
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