Activating Compound | Comment | Organism | Structure |
---|---|---|---|
allantoin | activation, sucrose synthesis | Glycine max |
General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | not inhibitory | Glycine max | |
ATP | sucrose synthesis | Glycine max | |
fructose | - |
Glycine max | |
glucose | - |
Glycine max | |
imidazole | - |
Glycine max | |
additional information | not inhibitory: DTT, GSH, 2-mercaptoethanol, EDTA; not inhibitory: fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 1,6-diphosphate; not inhibitory: galactose, mannose, maltose, raffinose, 3-phosphoglycerate, phosphoenolpyruvate, ethanol, succinate, 2-oxoglutarate, glutamine, NAD+, diphosphate | Glycine max | |
Tris | sucrose cleavage | Glycine max | |
UDP | strong | Glycine max | |
UDPglucose | - |
Glycine max | |
UTP | synthesis | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
UDP | - |
Glycine max | |
0.012 | 0.033 | UDPglucose | - |
Glycine max | |
0.13 | - |
ADP | - |
Glycine max | |
0.98 | 1.66 | fructose | cosubstrate CDP | Glycine max | |
0.98 | 1.66 | fructose | cosubstrate ADPglucose | Glycine max | |
3.7 | 5 | fructose | - |
Glycine max | |
16.9 | 40 | sucrose | - |
Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Glycine max | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation of sucrose synthesis | Glycine max | |
Ca2+ | slight activation of sucrose cleavage at 10 mM | Glycine max | |
Mg2+ | activation | Glycine max | |
Mg2+ | slight activation of sucrose cleavage | Glycine max | |
Mn2+ | activation | Glycine max | |
additional information | no activation by K+, Na+, NH4+, Cl-, Br-, F-, NO3-, phosphate, sulfate, borate, acetate, citrate | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
4 * 90000, SDS-PAGE | Glycine max |
380000 | - |
SDS-PAGE | Glycine max |
412000 | - |
gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
Merr cv. Williams | - |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nodule | nodulaid inoculum: Rhizobium japonicum CB 1809 | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.1 | - |
- |
Glycine max |
Storage Stability | Organism |
---|---|
4°C, 20 mM potassium phosphate buffer, pH 7.0, 5 mM 2-mercaptoethanol, 4 weeks stable | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + D-fructose | - |
Glycine max | ADP + sucrose | - |
r | |
CDP-glucose + D-fructose | - |
Glycine max | CDP + sucrose | - |
r | |
GDP-glucose + D-fructose | - |
Glycine max | GDP + sucrose | - |
r | |
UDP + sucrose | also substrate: ADP | Glycine max | UDP-glucose + D-fructose | - |
? | |
UDP + sucrose | substrate: CDP | Glycine max | UDP-glucose + D-fructose | - |
? | |
UDP + sucrose | ADP is less effective | Glycine max | UDP-glucose + D-fructose | - |
? | |
UDPglucose + D-fructose | - |
Glycine max | UDP + sucrose | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 90000, SDS-PAGE | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
sucrose cleavage | Glycine max |
9.5 | - |
sucrose synthesis | Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.2 | about 90% of maximal activity at pH 5.0 and about half-maximal activity at pH 8.2, sucrose cleavage | Glycine max |
7.5 | 10 | about half-maximal activity at pH 7.5 and about 90% of maximal activity at pH 10.0, sucrose synthesis | Glycine max |