Information on EC 2.4.1.13 - sucrose synthase

Word Map on EC 2.4.1.13
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.4.1.13
-
RECOMMENDED NAME
GeneOntology No.
sucrose synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
NDP-glucose + D-fructose = NDP + sucrose
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Starch and sucrose metabolism
-
-
sucrose degradation II (sucrose synthase)
-
-
non-pathway related
-
-
SYSTEMATIC NAME
IUBMB Comments
NDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) [10].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-05-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
kangaroo paw
-
-
Manually annotated by BRENDA team
Arachnis hookeriana x Ascocenda Madame Kenny
Mokara Yellow
-
-
Manually annotated by BRENDA team
var. pendula and var. carelica
-
-
Manually annotated by BRENDA team
2 genotypes ICPL 84023 and ICP 301 tolerant to waterlogging stress, and 2 genotypes ICP 7035 and Pusa 207 susceptible to waterlogging stress
-
-
Manually annotated by BRENDA team
green alga
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
inbred line M01-3
-
-
Manually annotated by BRENDA team
carnation
-
-
Manually annotated by BRENDA team
Gladiolus sp.
gladiolus
-
-
Manually annotated by BRENDA team
sweet potato, cv. Okinava no. 100
-
-
Manually annotated by BRENDA team
morning-glory
-
-
Manually annotated by BRENDA team
Leleba oldhami
bamboo
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
banana
-
-
Manually annotated by BRENDA team
quaking aspen
-
-
Manually annotated by BRENDA team
almond
-
-
Manually annotated by BRENDA team
var. Rehder var. culta Rehder cv. Hosui, isoforms SS I, SS II
-
-
Manually annotated by BRENDA team
L. Moench
-
-
Manually annotated by BRENDA team
sturt pea
-
-
Manually annotated by BRENDA team
green alga
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
GDP-glucose + D-fructose
GDP + sucrose
show the reaction diagram
IDP-glucose + D-fructose
IDP + sucrose
show the reaction diagram
-
-
-
-
?
NDP-glucose + D-fructose
NDP + sucrose
show the reaction diagram
sucrose + UDP
UDP-glucose + D-fructose
show the reaction diagram
sucrose loaded into the phloem of a poplar leaf is used directly by sucrose synthase associated with various beta-glucan synthases in the stem for UDPglucose formation in the sink tissue, thereby conserving the high energy bond between glucose and fructose
-
-
?
TDP-glucose + D-fructose
TDP + sucrose
show the reaction diagram
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
UDP-alpha-D-galactose + D-fructose
UDP + sucrose
show the reaction diagram
-
23% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-alpha-D-glucose + 1-deoxy-1-fluoro-fructose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 175% of the activity with D-fructose, 100% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-fructose
UDP + sucrose
show the reaction diagram
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
sucrose synthase plays an important role in sugar metabolism during sucrose accumulation in the coffee fruit
-
-
?
UDP-alpha-D-glucose + D-glucose
?
show the reaction diagram
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 2% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + D-lyxose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 150% of the activity with D-fructose, 48% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-mannose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 75% of the activity with D-fructose, 40% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-ribose
?
show the reaction diagram
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 7% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + D-ribulose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 24% of the activity with D-fructose, no activity with enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-tagatose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 43% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-talose
?
show the reaction diagram
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 117% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + D-xylose
?
show the reaction diagram
UDP-alpha-D-glucose + L-arabinose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 490% of the activity with D-fructose, 36% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-galactose
?
show the reaction diagram
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 2% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + L-glucose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 34% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-mannose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 59% of the activity with D-fructose, 8% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-rhamnose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 52% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-sorbose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 96% of the activity with D-fructose, 55% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucuronic acid + D-fructose
?
show the reaction diagram
-
32% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-alpha-D-xylose + D-fructose
?
show the reaction diagram
-
39% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
UDP-N-acetyl-alpha-D-galactosamine + D-fructose
UDP + beta-D-fructofuranosyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside
show the reaction diagram
-
23% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + D-fructose
UDP + 2-acetamido-2-deoxy-D-glucopyranosyl-beta-D-fructofuranoside
show the reaction diagram
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
NDP-glucose + D-fructose
NDP + sucrose
show the reaction diagram
sucrose + UDP
UDP-glucose + D-fructose
show the reaction diagram
Q01390
sucrose loaded into the phloem of a poplar leaf is used directly by sucrose synthase associated with various beta-glucan synthases in the stem for UDPglucose formation in the sink tissue, thereby conserving the high energy bond between glucose and fructose
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
UDP-alpha-D-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P10691
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
sucrose synthase plays an important role in sugar metabolism during sucrose accumulation in the coffee fruit
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
the transcript level and protein abundance of CsSUS3 hardly change during salinity treatment, but the enzyme activity increased slightly, especially on the first and ninth days, compared with control
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine
5-deoxy-beta-D-fructose
-
competitive to D-fructose
5-deoxy-D-fructose
10% inhibition
6-Dimethylaminopurine
-
sucrose-induced activity of sucrose synthase is strongly inhibited by okadaic acid and less by 6-dimethylaminopurine at early stages of regeneration, but not at the stages preceding DNA replication or mitotic activities
actin
-
0.001 mM, 17% inhibition of SuSy1
Ag+
-
at 0.4 mM
Arbutin
-
no enzyme activity at 5 mM
Co2+
-
0.05 mM, 20% loss of activity
D-fructose
Fe2+
-
no activity at 1 mM
Fe3+
-
0.4 mM
fructose
GDP
substrate inhibition occurs in the presence of GDP at concentrations above 10 mM
glucose
GTP
-
sucrose cleavage
imidazole
-
-
iodoacetic acid
N-ethylmaleimide
okadaic acid
-
sucrose-induced activity of sucrose synthase is strongly inhibited by okadaic acid and less by 6-dimethylaminopurine at early stages of regeneration, but not at the stages preceding DNA replication or mitotic activities
p-hydroxymercuribenzoate
Salicine
-
kinetics
Sucrose
UDP-glucose
UDPglucose
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
abscisic acid
-
as well as treatments like spikelet thinning, leaf cutting increase enzyme expression and enzyme activity during rice grain filling
actin
-
activity of SuSy2 in breakdown direction is stimulated by 60%
allantoin
-
activation, sucrose synthesis
Ca2+
-
only for sucrose synthesis
fructose 2,6-bisphosphate
-
stimulates SuSy2 in presence of actin
Mn2+
-
2-4fold activation of sucrose synthesis, inhibition of cleavage
nitrate
-
50% activation at 3-5 mM, exogenous nitrate at 14.2 mM absorbed in the form of KNO3 and Ca(NO3)2 during 10-20 days exponentially activates the enzyme in the roots by 22-100% as compared with plants grown on nitrogen-free medium. Under low light, nitrate can not activate sucrose synthase
Sucrose
-
exogenous, as well as treatments like spikelet thinning, leaf cutting increase enzyme expression and enzyme activity during rice grain filling
UDP-D-xylose
-
activation of epicotyl and cotyledon isozymes
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13 - 3.8
ADP
0.033 - 0.044
ADP-glucose
0.44 - 16.9
CDP
1.05 - 120
D-fructose
0.012
dTDP
-
pH 7.6, 30C
0.98 - 40
fructose
0.17 - 8.5
GDP
0.0916 - 321
Sucrose
0.65 - 1.4
TDP
0.00191 - 7.8
UDP
0.6 - 1.4
UDP-alpha-D-glucose
0.77
UDP-GlcNAc
-
pH 8.0, 30C
0.05 - 25
UDP-glucose
11.5
UDPfructose
-
isozyme SS1
0.012 - 6
UDPglucose
additional information
additional information
-