Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) pLysS cells | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-aminoethyl-L-cysteine | - |
Candida albicans | |
Cu2+ | strong inhibitory effect on isoform Lys22p | Candida albicans | |
DL-5-hydroxylysine | - |
Candida albicans | |
DL-alpha-Difluoromethyllysine | strong inhibitor of isoform Lys22p, but not Lys21p | Candida albicans | |
EDTA | strong inhibitory effect on isoform Lys22p | Candida albicans | |
L-Arg | inhibitor of isoform Lys22p, but not Lys21p | Candida albicans | |
L-Lys | competitive inhibitor | Candida albicans | |
additional information | not inhibited by L-ornithine, L-norleucine, L-leucine, L-canavanine, gamma-amino-beta-hydroxybutyric acid, gamma-amino-n-butyric acid, and O-(2-aminoethyl)-L-serine; there is little or no effect of Mg2+ or Ca2+ ions | Candida albicans | |
S-(2-aminoethyl)-L-cysteine | - |
Candida albicans | |
Zn2+ | strong inhibitory effect on isoform Lys22p | Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.113 | - |
2-oxoglutarate | isoform Lys21p, at pH 7.5 and 22°C | Candida albicans | |
0.152 | - |
2-oxoglutarate | isoform Lys22p, at pH 7.5 and 22°C | Candida albicans | |
0.48 | - |
acetyl-CoA | isoform Lys22p, at pH 7.5 and 22°C | Candida albicans | |
0.8 | - |
acetyl-CoA | isoform Lys21p, at pH 7.5 and 22°C | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47087 | - |
4 * 47087, isoform Lys22p, calculated from amino acid sequence | Candida albicans |
48300 | - |
4 * 48300, isoform Lys21p, SDS-PAGE | Candida albicans |
48314 | - |
4 * 48314, isoform Lys21p, calculated from amino acid sequence | Candida albicans |
49400 | - |
4 * 49400, isoform Lys22p, SDS-PAGE | Candida albicans |
200700 | - |
isoform Lys22p, gel filtration | Candida albicans |
210300 | - |
isoform Lys21p, gel filtration | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + 2-oxoglutarate | Candida albicans | - |
(2R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + 2-oxoglutarate | - |
Candida albicans | (2R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA | - |
? | |
additional information | isoforms Lys21p and Lys22p are not able to catalyze the condensation of oxaloacetate with acetyl-CoA to give citrate | Candida albicans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 47087, isoform Lys22p, calculated from amino acid sequence | Candida albicans |
homotetramer | 4 * 48300, isoform Lys21p, SDS-PAGE | Candida albicans |
homotetramer | 4 * 48314, isoform Lys21p, calculated from amino acid sequence | Candida albicans |
homotetramer | 4 * 49400, isoform Lys22p, SDS-PAGE | Candida albicans |
Synonyms | Comment | Organism |
---|---|---|
HCS | - |
Candida albicans |
LYS21 | - |
Candida albicans |
Lys21p | isoform | Candida albicans |
LYS22 | - |
Candida albicans |
Lys22p | isoform | Candida albicans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
acetyl-CoA | isoform Lys21p, at pH 7.5 and 22°C | Candida albicans | |
0.2 | - |
acetyl-CoA | isoform Lys22p, at pH 7.5 and 22°C | Candida albicans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.032 | - |
isoform Lys22p, at pH 7.5 and 22°C | Candida albicans | DL-alpha-Difluoromethyllysine | |
0.0541 | - |
isoform Lys22p, at pH 7.5 and 22°C | Candida albicans | L-Lys | |
0.286 | - |
isoform Lys21p, at pH 7.5 and 22°C | Candida albicans | L-Lys | |
1.8 | - |
isoform Lys22p, at pH 7.5 and 22°C | Candida albicans | S-(2-aminoethyl)-L-cysteine | |
5.7 | - |
isoform Lys22p, at pH 7.5 and 22°C | Candida albicans | DL-5-hydroxylysine | |
16.5 | - |
isoform Lys21p, at pH 7.5 and 22°C | Candida albicans | 2-aminoethyl-L-cysteine | |
18.7 | - |
isoform Lys22p, at pH 7.5 and 22°C | Candida albicans | L-Arg |