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Literature summary for 2.3.1.82 extracted from

  • Hamano, Y.; Hoshino, Y.; Nakamori, S.; Takagi, H.
    Overexpression and characterization of an aminoglycoside 6'-N-acetyltransferase with broad specificity from an epsilon-poly-L-lysine producer, Streptomyces albulus IFO14147 (2004), J. Biochem., 136, 517-524.
    View publication on PubMed
Search for more literature for 2.3.1.82

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces albulus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00038
-
 dibekacin pH 8.0, 30°C Streptomyces albulus
0.00052
-
 neomycin B pH 8.0, 30°C Streptomyces albulus
0.00324
-
 sisomicin pH 8.0, 30°C Streptomyces albulus
0.00353
-
 tobramycin pH 8.0, 30°C Streptomyces albulus
0.00434
-
 amikacin pH 8.0, 30°C Streptomyces albulus
0.0074
-
 kanamycin A pH 8.0, 30°C Streptomyces albulus
0.0161
-
 n-propionyl-CoA pH 8.0, 30°C, cosubstrate: sisomycin Streptomyces albulus
0.0187
-
 acetyl-CoA pH 8.0, 30°C, cosubstrate: sisomycin Streptomyces albulus
0.04188
-
 lividomycin A pH 8.0, 30°C Streptomyces albulus
0.0686
-
 n-butyryl-CoA pH 8.0, 30°C, cosubstrate: sisomycin Streptomyces albulus
0.58
-
 malonyl-CoA pH 8.0, 30°C, cosubstrate: sisomycin Streptomyces albulus

Organism

Organism UniProt Comment Textmining
Streptomyces albulus Q764C8 IFO14147
-
Streptomyces albulus IFO14147 Q764C8 IFO14147
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged AAC(6')-Isa Streptomyces albulus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + amikacin
-
 Streptomyces albulus CoA + N6'-acetylamikacin
-
 ?
acetyl-CoA + amikacin
-
 Streptomyces albulus IFO14147 CoA + N6'-acetylamikacin
-
 ?
acetyl-CoA + dibekacin
-
 Streptomyces albulus CoA + N6'-acetyldibekacin
-
 ?
acetyl-CoA + dibekacin
-
 Streptomyces albulus IFO14147 CoA + N6'-acetyldibekacin
-
 ?
acetyl-CoA + kanamycin A
-
 Streptomyces albulus CoA + N6'-acetylkanamycin A
-
 ?
acetyl-CoA + kanamycin A
-
 Streptomyces albulus IFO14147 CoA + N6'-acetylkanamycin A
-
 ?
acetyl-CoA + lividomycin A
-
 Streptomyces albulus CoA + O6'-acetyllividomycin A
-
 ?
acetyl-CoA + neomycin B
-
 Streptomyces albulus CoA + ?
-
 ?
acetyl-CoA + sisomicin
-
 Streptomyces albulus CoA + N6'-acetylsisomicin
-
 ?
acetyl-CoA + tobramycin
-
 Streptomyces albulus CoA + N6'-acetyltobramycin
-
 ?
acetyl-CoA + tobramycin
-
 Streptomyces albulus IFO14147 CoA + N6'-acetyltobramycin
-
 ?
malonyl-CoA + sisomycin
-
 Streptomyces albulus CoA + ?
-
 ?
additional information the enzyme is susceptoble to mild base hydrolysis, suggesting that the enzyme also catalyzes O-acetyltransfer Streptomyces albulus ?
-
 ?
additional information the enzyme is susceptoble to mild base hydrolysis, suggesting that the enzyme also catalyzes O-acetyltransfer Streptomyces albulus IFO14147 ?
-
 ?
n-butyryl-CoA + sisomycin
-
 Streptomyces albulus CoA + ?
-
 ?
n-propionyl-CoA + sisomycin
-
 Streptomyces albulus CoA + ?
-
 ?

Synonyms

Synonyms Comment Organism
AAC(6')-Isa
-
 Streptomyces albulus
aminoglycoside 6'-N-acetyltransferase
-
 Streptomyces albulus