Information on EC 2.3.1.82 - aminoglycoside 6'-N-acetyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.82
-
RECOMMENDED NAME
GeneOntology No.
aminoglycoside 6'-N-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + kanamycin-B = CoA + N6'-acetylkanamycin-B
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:kanamycin-B N6'-acetyltransferase
The antibiotics kanamycin A, kanamycin B, neomycin, gentamicin C1a, gentamicin C2 and sisomicin are substrates. The antibiotic tobramycin, but not paromomycin, can also act as acceptor. The 6-amino group of the purpurosamine ring is acetylated.
CAS REGISTRY NUMBER
COMMENTARY hide
56467-65-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 8
-
-
Manually annotated by BRENDA team
strain 8
-
-
Manually annotated by BRENDA team
strain CFr564, enzyme variant with Ser119
-
-
Manually annotated by BRENDA team
strain CFr564, enzyme variant with Ser119
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain EC1562 and EC1563, enzyme variant with Ser119
-
-
Manually annotated by BRENDA team
strain EC1562 and EC1563, enzyme variant with Ser119
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
CS2R2
-
-
Manually annotated by BRENDA team
NR79
-
-
Manually annotated by BRENDA team
strains 2513 and 731
-
-
Manually annotated by BRENDA team
strain BM2687, enzyme Ib‘
-
-
Manually annotated by BRENDA team
strain BM2687, enzyme Ib‘
-
-
Manually annotated by BRENDA team
strain VU12944/77
-
-
Manually annotated by BRENDA team
RYC 13036
-
-
Manually annotated by BRENDA team
bifunctional enzyme, with a N-terminal coenzyme A-dependent acetyltransferase domain and a C-terminal GTP-dependent phosphotransferase domain
UniProt
Manually annotated by BRENDA team
IFO14147
SwissProt
Manually annotated by BRENDA team
IFO14147
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 2'-N-ethylnetilmicin
CoA + N6'-acetyl-2'-N-ethylnetilmicin
show the reaction diagram
acetyl-CoA + amikacin
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + amikacin
CoA + 6'-N-acetylamikacin
show the reaction diagram
acetyl-CoA + amikacin
CoA + N6'-acetylamikacin
show the reaction diagram
acetyl-CoA + aminoglycoside
CoA + 6'-N-acetylaminoglycoside
show the reaction diagram
-
-
-
-
?
acetyl-CoA + arbekacin
CoA + 6'-N-acetylarbekacin
show the reaction diagram
acetyl-CoA + arbekacin
CoA + N6'-acetylarbekacin
show the reaction diagram
-
-
-
?
acetyl-CoA + butirosin
CoA + N6'-acetylbutirosin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + ciprofloxacin
CoA + N4'-acetylnorfloxacin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + dibekacin
CoA + 6'-N-acetyldibekacin
show the reaction diagram
acetyl-CoA + dibekacin
CoA + N6'-acetyldibekacin
show the reaction diagram
acetyl-CoA + fortimicin A
CoA + N6'-acetylfortimicin A
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin
CoA + 6'-N-acetylgentamicin
show the reaction diagram
acetyl-CoA + gentamicin
CoA + N6'-acetylgentamicin
show the reaction diagram
acetyl-CoA + gentamicin B
CoA + N6'-acetylgentamicin B
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin C
CoA + N6'-acetylgentamicin C
show the reaction diagram
acetyl-CoA + gentamicin C1
CoA + N6'-acetylgentamicin C1
show the reaction diagram
-
the AAC(6')-Ib protein is unable to efficiently modify gentamicin C1, 1.7% relative activity to sisomicin, however it is capable of modifying amikacin, 65.5% relative activity to sisomycin. The mutant enzyme AAC(6')-Ib L119S shows a 2.8fold increase in acetylation of gentamicin C1, but a 8.7fold reduction in the ability to modify amikacin. The AAC(6')-IIa protein modifies gentamicin C1 at 10.1% relative activity to sisomicin, however it shows low activity towards amikacin, 4.1% activity relative to sisomycin. The mutation AAC(6')-IIa S119L results in a 4.8fold reduction in the acetylation of gentamicin C1, but causes an 2fold increase in the ability to modify amikacin
-
-
?
acetyl-CoA + gentamicin C1a
CoA + N6'-acetylgentamicin C1a
show the reaction diagram
acetyl-CoA + gentamicin C2
CoA + N6'-acetylgentgamicin C2
show the reaction diagram
-
poor substrate, not gentamicin A and C1
-
-
?
acetyl-CoA + histone
CoA + ?
show the reaction diagram
a mixture of calf histones enriched in H3 and H4
-
-
?
acetyl-CoA + human histone H3 peptide containing a C-terminal cysteine residue
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hybrimycin A1
CoA + N6'-acetylhybrimycin A1
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hybrimycin A2
CoA + N6'-acetylhybrimycin A2
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hybrimycin A3
CoA + N6'-acetylhybrimycin A3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hybrimycin B1
CoA + N6'-acetylhybrimycin B1
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hybrimycin B2
CoA + N6'-acetylhybrimycin B2
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hybrimycin B3
CoA + N6'-acetylhybrimycin B3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isepamicin
CoA + 6'-N-acetylisepamicin
show the reaction diagram
acetyl-CoA + isepamicin
CoA + N6'-acetylisepamicin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + istamycin-B
CoA + N6'-acetylistamycin-B
show the reaction diagram
-
-
-
-
?
acetyl-CoA + kanamycin
CoA + 6'-N-acetylkanamycin
show the reaction diagram
acetyl-CoA + kanamycin
CoA + N6'-acetylkanamycin
show the reaction diagram
acetyl-CoA + kanamycin A
CoA + N6'-acetylkanamycin A
show the reaction diagram
acetyl-CoA + kanamycin B
CoA + N6'-acetylkanamycin B
show the reaction diagram
acetyl-CoA + lividomycin A
CoA + O6'-acetyllividomycin A
show the reaction diagram
-
-
-
?
acetyl-CoA + micromomicin
CoA + N6'-acetylmicromomicin
show the reaction diagram
acetyl-CoA + myelin basic protein
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + neamine
CoA + 6'-N-acetylneamine
show the reaction diagram
acetyl-CoA + neamine
CoA + N6'-acetylneamine
show the reaction diagram
acetyl-CoA + nebramycin factor 4
CoA + N6'-acetylnebramycin factor 4
show the reaction diagram
-
no activity with factor 2
-
-
?
acetyl-CoA + nebramycin factor 6
CoA + N6'-acetylnebramycin factor 6
show the reaction diagram
-
no activity with factor 2
-
-
?
acetyl-CoA + neomycin
CoA + 6'-N-acetylneomycin
show the reaction diagram
acetyl-CoA + neomycin
CoA + N6'-acetylneomycin
show the reaction diagram
acetyl-CoA + neomycin A
CoA + N6'-acetylneomycin A
show the reaction diagram
-
-
-
-
?
acetyl-CoA + neomycin B
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + neomycin B
CoA + N6'-acetylneomycin B
show the reaction diagram
acetyl-CoA + neomycin C
CoA + N6'-acetylneomycin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + neomycin C
CoA + N6'-acetylneomycin C
show the reaction diagram
acetyl-CoA + netilmicin
CoA + 6'-N-acetylnetilmicin
show the reaction diagram
acetyl-CoA + netilmicin
CoA + N6'-acetylnetilmicin
show the reaction diagram
acetyl-CoA + norfloxacin
CoA + N4'-acetylnorfloxacin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + paromomycin
CoA + O6'-acetylparomomycin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + poly-L-Lys
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + ribostamycin
CoA + 6'-N-acetylribostamycin
show the reaction diagram
acetyl-CoA + ribostamycin
CoA + N6'-acetylribostamycin
show the reaction diagram
acetyl-CoA + ribostamycin
CoA + N6'acetylribostamycin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sisomicin
CoA + 6'-N-acetylsisomicin
show the reaction diagram
acetyl-CoA + sisomicin
CoA + N6'-acetylsisomicin
show the reaction diagram
acetyl-CoA + tobramicin
CoA + N6'-acetyltobramicin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + tobramycin
CoA + 6'-N-acetyltobramycin
show the reaction diagram
acetyl-CoA + tobramycin
CoA + N6'-acetyltobramycin
show the reaction diagram
butyryl-CoA + tobramycin
CoA + N6'-butyryltobramycin
show the reaction diagram
-
-
-
-
?
malonyl-CoA + sisomycin
CoA + ?
show the reaction diagram
-
-
-
?
malonyl-CoA + tobramycin
CoA + N6'-malonyltobramycin
show the reaction diagram
-
-
-
-
?
n-butyryl-CoA + sisomycin
CoA + ?
show the reaction diagram
-
-
-
?
n-propionyl-CoA + sisomycin
CoA + ?
show the reaction diagram
-
-
-
?
propionyl-CoA + tobramycin
CoA + N6'-propionyltobramycin
show the reaction diagram
-
tobramycin exhibits a rapid, tobramycin-independent rate of hydrolysis that is linearly proportional to enzyme
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + aminoglycoside
CoA + 6'-N-acetylaminoglycoside
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetyl-CoA
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[(3-sulfanylpropanoyl)amino]-alpha-D-glucopyranoside-S-CoA
-
-
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[(4-sulfanylbutanoyl)amino]-alpha-D-glucopyranoside-S-CoA
-
-
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[(5-sulfanylpentanoyl)amino]-alpha-D-glucopyranoside-S-CoA
-
-
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[(sulfanylacetyl)amino]-alpha-D-glucopyranoside-S-CoA
-
-
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[[(2-sulfanylethyl)sulfonyl]amino]-alpha-D-glucopyranoside-S-CoA
-
-
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[[(dioxidosulfanyl)acetyl]amino]-alpha-D-glucopyranoside-S-CoA
-
-
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[[(oxidosulfanyl)acetyl]amino]-alpha-D-glucopyranoside-S-CoA
-
-
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[[3-(dioxidosulfanyl)propanoyl]amino]-alpha-D-glucopyranoside-S-CoA
-
-
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[[3-(oxidosulfanyl)propanoyl]amino]-alpha-D-glucopyranoside-S-CoA
-
-
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2-deoxy-6-O-[methoxy(2-sulfanylethyl)phosphoryl]-alpha-D-glucopyranoside-S-CoA
-
-
1-(bromomethyl)phenanthrene
-
inactivates through covalent modification of Asp99
2-[5-[(4,6-dioxo-1,3-diphenyl-2-thioxotetrahydro-5(2H)-pyrimidinylidene)methyl]-2-furyl]benzoic acid
identified by in silico molecular docking, micromolar activity in inhibiting acetylation of kanamycin A
-
4-[(2-[[1-(3-methylphenyl)-4,6-dioxo-2-thioxotetrahydro-5(2H)-pyrimidinylidene]methyl]phenoxy)methyl]benzoic acid
identified by in silico molecular docking, micromolar activity in inhibiting acetylation of kanamycin A
6'-N-acetylkanamycin A
-
competitive versus kanamycin A and noncompetitive/mixed versus acetyl-CoA
6'-N-acetylneamine
-
product inhibition
amikacin
-
substrate inhibition
butyryl-CoA
CoA
-
product inhibition
CoA-aminoglycoside 1
-
-
CoA-aminoglycoside 11a
-
-
CoA-aminoglycoside 11b
-
-
CoA-aminoglycoside 11c
-
-
CoA-aminoglycoside 1a
-
-
CoA-aminoglycoside 1b
-
-
CoA-aminoglycoside 1c
-
-
CoA-aminoglycoside 1d
-
-
CoA-aminoglycoside 2
-
-
CoA-aminoglycoside 3
-
-
CoA-aminoglycoside 4a
-
competitive
CoA-aminoglycoside 4b
-
competitive
CoA-aminoglycoside 4c
-
competitive
CoA-aminoglycoside 4d
-
competitive
CoA-aminoglycoside 4e
-
competitive
CoA-aminoglycoside 4f
-
competitive
CoA-aminoglycoside 5b
-
competitive
CoA-aminoglycoside 5d
-
competitive
CoA-aminoglycoside 5e
-
competitive
CoASH
Cu2+
the growth of an amikacin-resistant Klebsiella pneumoniae strain is inhibited when amikacin is supplemented by the addition of Zn2+ or Cu2+ in complex with the ionophore pyrithione
dithio-CoA
-
-
gentamicin
-
substrate inhibition
gentamicin A
-
-
gentamicin C1
gentamine A
-
-
iodoacetamide
-
inactivation in a biphasic manner, half of the activity is lost rapidly and the other half more slowly, tobramycin but not acetyl-CoA protects
kanamycin A
-
-
kanamycin B
-
-
lividomycin
-
-
lividomycin A
-
-
N6'-acetyl kanamycin A
-
-
N6'-Acetylgentamicin C1a
-
-
N6'-acetylneomycin B
-
-
netilmicin
paromamine
-
-
paromomycin
Pefloxacin
-
-
poly-L-Lys
substrate inhibition
tobramycin
additional information
-
no inhibition by GTP
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0033 - 0.215
acetyl-CoA
0.00055 - 2.19
amikacin
0.036
arbekacin
pH not specified in the publication, temperature not specified in the publication
-
0.014
butirosin
-
pH 6.0, 37°C
9.3
butyryl-CoA
-
reaction with tobramycin, wild-type enzyme
0.07
Ciprofloxacin
-
AAC(6')-Ib-cr variant
0.00038 - 0.036
dibekacin
0.0254 - 0.07
fortimicin A
0.00504 - 0.653
gentamicin
0.0223
gentamicin B
-
pH 6.0, 37°C
0.0082
gentamicin C
-
wild-type enzyme
0.0003 - 0.044
isepamicin
0.00202 - 0.154
kanamycin
0.001 - 3.49
kanamycin A
0.00027 - 0.9
kanamycin B
0.04188
lividomycin A
pH 8.0, 30°C
0.58 - 0.82
malonyl-CoA
0.0686
n-Butyryl-CoA
pH 8.0, 30°C, cosubstrate: sisomycin
0.0161
n-propionyl-CoA
pH 8.0, 30°C, cosubstrate: sisomycin
0.0058 - 0.509
neamine
0.0012 - 0.0573
neomycin
0.53
neomycin A
-
-
0.00052 - 0.02
neomycin B
0.00531 - 0.25
neomycin C
0.001 - 2.44
netilmicin
0.067
Norfloxacin
-
AAC(6')-Ib-cr variant
0.324 - 0.771
paromomycin
0.038
poly-L-Lys
pH 7.5, 37°C
0.0195 - 0.27
propionyl-CoA
0.00908 - 0.306
ribostamycin
0.0022 - 0.012
sisomicin
0.216
tobramicin
-
pH 5.5, 37°C
0.00353 - 1.15
tobramycin
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01 - 12.1
acetyl-CoA
0.003 - 7.09
amikacin
0.57
arbekacin
Stenotrophomonas maltophilia
V5XKM9
pH not specified in the publication, temperature not specified in the publication
-
0.466
butirosin
Enterococcus faecium
-
pH 6.0, 37°C
1.03
Ciprofloxacin
Escherichia coli
-
AAC(6')-Ib-cr variant
0.25 - 1.3
dibekacin
0.0002 - 0.45
fortimicin A
0.5 - 3.33
gentamicin
0.388
gentamicin B
Enterococcus faecium
-
pH 6.0, 37°C
1.01
gentamicin C
Enterococcus faecium
-
wild-type enzyme
0.05 - 2.4
isepamicin
0.04 - 3.81
kanamycin
0.01 - 2.4
kanamycin A
1.08 - 5.25
kanamycin B
0.002 - 1.3
neamine
0.001 - 11.1
neomycin
3.08
neomycin B
Escherichia coli
-
AAC(6')-Ib, wild type
0.135 - 0.205
neomycin C
0.2 - 3.21
netilmicin
1.08
Norfloxacin
Escherichia coli
-
AAC(6')-Ib-cr variant
0.0004 - 0.059
paromomycin
0.0019
poly-L-Lys
Enterococcus faecium
Q47764
pH 7.5, 37°C
0.317
propionyl-CoA
Enterococcus faecium
-
pH 6.0, 37°C
0.01 - 5.7
ribostamycin
0.16 - 5.35
sisomicin
0.004 - 3.84
tobramycin
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.4 - 82
acetyl-CoA
29
4 - 5250
amikacin
1394
16
arbekacin
Stenotrophomonas maltophilia
V5XKM9
pH not specified in the publication, temperature not specified in the publication
206872
10
dibekacin
Stenotrophomonas maltophilia
V5XKM9
pH not specified in the publication, temperature not specified in the publication
6512
220 - 234
gentamicin
2406
1
isepamicin
Stenotrophomonas maltophilia
V5XKM9
pH not specified in the publication, temperature not specified in the publication
36503
1 - 189
kanamycin
5165
34 - 394
neomycin
1591
4 - 1080
netilmicin
2604
82.2 - 243
ribostamycin
1627
38 - 1330
sisomicin
3365
6 - 485
tobramycin
939
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00004
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[(3-sulfanylpropanoyl)amino]-alpha-D-glucopyranoside-S-CoA
-
-
0.00016
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[(4-sulfanylbutanoyl)amino]-alpha-D-glucopyranoside-S-CoA
-
-
0.008
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[(5-sulfanylpentanoyl)amino]-alpha-D-glucopyranoside-S-CoA
-
-
0.00008
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[(sulfanylacetyl)amino]-alpha-D-glucopyranoside-S-CoA
-
-
0.0016
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[[(2-sulfanylethyl)sulfonyl]amino]-alpha-D-glucopyranoside-S-CoA
0.00027
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[[(dioxidosulfanyl)acetyl]amino]-alpha-D-glucopyranoside-S-CoA
-
competitive inhibition
0.00006
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[[(oxidosulfanyl)acetyl]amino]-alpha-D-glucopyranoside-S-CoA
-
competitive inhibition
0.00009
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[[3-(dioxidosulfanyl)propanoyl]amino]-alpha-D-glucopyranoside-S-CoA
-
competitive inhibition
0.002
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2,6-dideoxy-6-[[3-(oxidosulfanyl)propanoyl]amino]-alpha-D-glucopyranoside-S-CoA
-
competitive inhibition
0.002
(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2-deoxy-6-O-[methoxy(2-sulfanylethyl)phosphoryl]-alpha-D-glucopyranoside-S-CoA
-
-
0.04 - 0.55
6'-N-acetylated kanamycin A
0.04 - 0.385
6'-N-acetylkanamycin A
0.00499 - 0.02206
amikacin
6
AMP
-
pH 6.9, 37°C, inhibition of the reaction with neamine
0.025 - 0.57
butyryl-CoA
0.0036
CoA
-
pH 6.9, 37°C
0.000076
CoA-aminoglycoside 1
-
-
0.000043
CoA-aminoglycoside 11a
-
-
0.000161
CoA-aminoglycoside 11b
-
-
0.00799
CoA-aminoglycoside 11c
-
-
0.000076
CoA-aminoglycoside 1a
-
-
0.000043
CoA-aminoglycoside 1b
-
-
0.000161
CoA-aminoglycoside 1c
-
-
0.008
CoA-aminoglycoside 1d
-
-
0.00011 - 0.000111
CoA-aminoglycoside 2
0.000119
CoA-aminoglycoside 3
-
-
0.0034
CoA-aminoglycoside 4a
-
-
0.13
CoA-aminoglycoside 4b
-
-
0.0012
CoA-aminoglycoside 4c
-
-
0.0034
CoA-aminoglycoside 4d
-
-
0.0036
CoA-aminoglycoside 4e
-
-
0.0074
CoA-aminoglycoside 4f
-
-
0.012
CoA-aminoglycoside 5b
-
-
0.0022
CoA-aminoglycoside 5d
-
-
0.011
CoA-aminoglycoside 5e
-
-
0.02 - 0.2
CoASH
0.072
dibekacin
-
pH 6.0, 37°C
0.02256 - 0.079
gentamicin
4
gentamicin C1
-
pH 6.9, 37°C, inhibition of the reaction with neamine
0.196