Application | Comment | Organism |
---|---|---|
medicine | understanding of biosynthetic pathway of taxol, target: possibly biological production of taxol, taxol or paclitaxel is now well established as a potent chemotherapeutic drug | Taxus cuspidata |
medicine | understanding of biosynthetic pathway of taxol, target: possibly biological production of taxol, taxol or paclitaxel is now well established as a potent chemotherapeutic drug | Taxus canadensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | weak | Taxus canadensis | |
CoA | weak | Taxus cuspidata | |
N-ethylmaleimide | weak | Taxus canadensis | |
N-ethylmaleimide | weak | Taxus cuspidata | |
p-hydroxymercuribenzoate | weak | Taxus canadensis | |
p-hydroxymercuribenzoate | weak | Taxus cuspidata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
taxa-4(20),11-dien-5alpha-ol | - |
Taxus cuspidata | |
0.0042 | - |
taxa-4(20),11-dien-5alpha-ol | - |
Taxus canadensis | |
0.0055 | - |
acetyl-CoA | - |
Taxus cuspidata | |
0.0055 | - |
acetyl-CoA | - |
Taxus canadensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | monovalent and divalent cations show no effect on the enzyme | Taxus cuspidata | |
additional information | monovalent and divalent cations show no effect on the enzyme | Taxus canadensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel chromatography | Taxus cuspidata |
50000 | - |
gel chromatography | Taxus canadensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Taxus cuspidata | catalyzes the first acylation step of taxol biosynthesis | ? | - |
? | |
additional information | Taxus canadensis | catalyzes the first acylation step of taxol biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taxus canadensis | - |
methyl jasmonate-induced cells | - |
Taxus cuspidata | - |
methyl jasmonate-induced cells | - |
Purification (Comment) | Organism |
---|---|
partially | Taxus cuspidata |
partially | Taxus canadensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + farnesol | - |
Taxus cuspidata | CoA + ? | - |
? | |
acetyl-CoA + geranylgeraniol | - |
Taxus cuspidata | CoA + ? | - |
? | |
acetyl-CoA + phytol | - |
Taxus cuspidata | CoA + ? | - |
? | |
acetyl-CoA + taxa-4(20),11(12)-dien-5alpha-ol | - |
Taxus cuspidata | CoA + taxa-4(20),11-dien-5alpha-yl acetate | - |
? | |
acetyl-CoA + taxa-4(20),11(12)-dien-5alpha-ol | - |
Taxus canadensis | CoA + taxa-4(20),11-dien-5alpha-yl acetate | - |
? | |
acetyl-CoA + taxadienol | - |
Taxus cuspidata | CoA + ? | - |
? | |
additional information | catalyzes the first acylation step of taxol biosynthesis | Taxus cuspidata | ? | - |
? | |
additional information | catalyzes the first acylation step of taxol biosynthesis | Taxus canadensis | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
pI: 4.7 | Taxus cuspidata |
9 | - |
pI: 4.7 | Taxus canadensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | 50% of maximal activity at pH 7.50 and pH 10.0, enzyme inactive at pH 11.0 | Taxus cuspidata |
7.5 | 10 | 50% of maximal activity at pH 7.50 and pH 10.0, enzyme inactive at pH 11.0 | Taxus canadensis |