Information on EC 2.3.1.162 - taxadien-5alpha-ol O-acetyltransferase

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The expected taxonomic range for this enzyme is: Taxus

EC NUMBER
COMMENTARY hide
2.3.1.162
-
RECOMMENDED NAME
GeneOntology No.
taxadien-5alpha-ol O-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + taxa-4(20),11-dien-5alpha-ol = CoA + taxa-4(20),11-dien-5alpha-yl acetate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
taxol biosynthesis
-
-
Diterpenoid biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:taxa-4(20),11-dien-5alpha-ol O-acetyltransferase
This is the third enzyme in the biosynthesis of the diterpenoid antineoplastic drug taxol (paclitaxel), which is widely used in the treatment of carcinomas, sarcomas and melanomas.
CAS REGISTRY NUMBER
COMMENTARY hide
229032-29-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
Taxus sp.
-
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + farnesol
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + geranylgeraniol
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + phytol
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + taxa-4(20),11(12)-dien-5alpha-ol
CoA + taxa-4(20),11-dien-5alpha-yl acetate
show the reaction diagram
acetyl-CoA + taxadien-2alpha,5alpha,10beta-triol
CoA + taxadien-10beta-acetoxy-2alpha,5alpha-diol
show the reaction diagram
-
-
-
?
acetyl-CoA + taxadien-2alpha,5alpha,10beta-triol
CoA + taxadien-5alpha-acetoxy-2alpha,10beta-diol
show the reaction diagram
Taxus sp.
-
-
-
?
acetyl-CoA + taxadien-2alpha,5alpha-diol
CoA + taxadien-5alpha-acetoxy-2alpha-ol
show the reaction diagram
Taxus sp.
-
-
-
?
acetyl-CoA + taxadien-5alpha,9alpha,10beta,13alpha-tetraol
CoA + taxadien-10beta-acetoxy-5alpha,9alpha,13alpha-triol
show the reaction diagram
-
-
-
?
acetyl-CoA + taxadien-5alpha,9alpha,10beta,13alpha-tetraol
CoA + taxadien-13alpha-acetoxy-5alpha,9alpha,10beta-triol
show the reaction diagram
Taxus sp.
-
-
-
?
acetyl-CoA + taxadien-5alpha-acetoxy-9alpha,10beta,13alpha-triol + H2O
CoA + taxadien-5alpha,13alpha-diacetoxy-9alpha,10beta-diol
show the reaction diagram
Taxus sp.
-
-
-
?
acetyl-CoA + taxadien-5alpha-acetoxy-9alpha,10beta,13alpha-triol + H2O
CoA + taxadien-5alpha,9alpha,10beta-triacetoxy-13alpha-ol
show the reaction diagram
-
-
-
?
acetyl-CoA + taxadien-5alpha-ol + H2O
CoA + taxadien-5alpha-yl acetate
show the reaction diagram
acetyl-CoA + taxadienol
CoA + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-ethylmaleimide
p-hydroxymercuribenzoate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0055
acetyl-CoA
0.0042
taxa-4(20),11-dien-5alpha-ol
0.0129 - 0.0415
taxadien-2alpha,5alpha,10beta-triol
0.0239
taxadien-2alpha,5alpha-diol
Taxus sp.
-
0.0173 - 0.0453
taxadien-5alpha,9alpha,10beta,13alpha-tetraol
0.0835 - 0.2794
taxadien-5alpha-acetoxy-9alpha,10beta,13alpha-triol
0.005 - 0.0086
taxadien-5alpha-ol
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.77 - 27.92
taxadien-2alpha,5alpha,10beta-triol
52.13
taxadien-2alpha,5alpha-diol
Taxus sp.
Q5Y9C6
-
11.91 - 15.47
taxadien-5alpha,9alpha,10beta,13alpha-tetraol
145 - 297.9
taxadien-5alpha-acetoxy-9alpha,10beta,13alpha-triol
4.28 - 29.59
taxadien-5alpha-ol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 10
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4
calculation from nucleotide sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
strong expression
Manually annotated by BRENDA team
weak expression
Manually annotated by BRENDA team
additional information
no expression in fruits
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49080
-
recombinant enzyme, calculated from amino acid composition
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
800fold purification
-
partially
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
reconstruction of taxoid biosynthesis in Saccharomyces cerevisiae by simultaneous expression of five early pathway steps starting from isoprenoid precursors. Production of taxadiene reaches levels of 0.7 mg/l in YPG media and 1.0 mg/l in selective media
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine