Cloned (Comment) | Organism |
---|---|
wild-type enzyme and mutant enzymes C155S/C158S, C41S/C44S and C173S/C178S are expressed in Escherichi coli | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide | pH 7.0, 60°C | Saccharolobus solfataricus | |
0.00017 | - |
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide | pH 7.0, 60°C | Saccharolobus solfataricus | |
0.0002 | - |
[protein disulfide oxidoreductase]-disulfide | pH 7.0, 60°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97W27 | - |
- |
Saccharolobus solfataricus P2 | Q97W27 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus | [C173S/C178S mutant of protein-disulfide-oxidoreductase]-dithiol + NADP+ | - |
? | |
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus P2 | [C173S/C178S mutant of protein-disulfide-oxidoreductase]-dithiol + NADP+ | - |
? | |
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus | [C173S/C178S mutant ofprotein-disulfide-oxidoreductase]-dithiol + NADP+ | - |
? | |
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus P2 | [C173S/C178S mutant ofprotein-disulfide-oxidoreductase]-dithiol + NADP+ | - |
? | |
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus | [C41S/C44S mutant of protein disulfide oxidoreductase]-dithiol + NADP+ | - |
? | |
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus P2 | [C41S/C44S mutant of protein disulfide oxidoreductase]-dithiol + NADP+ | - |
? | |
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus | [C41S/C44S mutant ofprotein disulfide oxidoreductase]-dithiol + NADP+ | - |
? | |
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus P2 | [C41S/C44S mutant ofprotein disulfide oxidoreductase]-dithiol + NADP+ | - |
? | |
[protein disulfide oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus | [protein disulfide oxidoreductase]-dithiol + NADP+ | - |
? | |
[protein disulfide oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus P2 | [protein disulfide oxidoreductase]-dithiol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SSO2416 | locus name | Saccharolobus solfataricus |
SsTR | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Saccharolobus solfataricus |