Information on EC 1.8.1.B4 - [protein disulfide oxidoreductase]-disulfide oxidoreductase

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The expected taxonomic range for this enzyme is: Sulfolobus solfataricus

EC NUMBER
COMMENTARY hide
1.8.1.B4
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
[protein disulfide oxidoreductase]-disulfide oxidoreductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[protein disulfide oxidoreductase]-dithiol + NADP+ = [protein disulfide oxidoreductase]-disulfide + NADPH + H+
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
[protein disulfide oxidoreductase]:NADP+ oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-dithiol + NADP+
show the reaction diagram
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+
[C173S/C178S mutant ofprotein-disulfide-oxidoreductase]-dithiol + NADP+
show the reaction diagram
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+
[C41S/C44S mutant of protein disulfide oxidoreductase]-dithiol + NADP+
show the reaction diagram
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+
[C41S/C44S mutant ofprotein disulfide oxidoreductase]-dithiol + NADP+
show the reaction diagram
[protein disulfide oxidoreductase]-disulfide + NADPH + H+
[protein disulfide oxidoreductase]-dithiol + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[protein disulfide oxidoreductase]-disulfide + NADPH + H+
[protein disulfide oxidoreductase]-dithiol + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
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the highest activity is observed with protein disulfide oxidoreductase (SsPDO) as a substrate in the presence of FAD and NADPH as cofactors
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00012
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide
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pH 7.0, 60°C
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0.00017
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide
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pH 7.0, 60°C
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0.0002 - 0.0003
[protein disulfide oxidoreductase]-disulfide
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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wild-type enzyme and mutant enzymes C155S/C158S, C41S/C44S and C173S/C178S are expressed in Escherichi coli
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