General Stability | Organism |
---|---|
dithiothreitol stabilizes | Escherichia coli |
FAD, stabilizes | Escherichia coli |
glycerol stabilizes | Escherichia coli |
NADP+ stabilizes | Escherichia coli |
pure enzyme heated in presence of bovine serum albumin and 0.020 mM NADPH: loss of activity beyond 30°C, significant reduction even at 0°C | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
0.016 | - |
NADPH | - |
Escherichia coli | |
0.066 | - |
GSSG | - |
Escherichia coli | |
0.377 | - |
NADH | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000 | Escherichia coli |
109000 | - |
- |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH | Escherichia coli | - |
glutathione + NADP+ | - |
r | |
GSSG + NADPH | Escherichia coli S33 | - |
glutathione + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli S33 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
361 | - |
purified enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-18°C, in the dark, 10% glycerol, 5 mM NADP+, for at least 1 year | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADH + H+ | - |
Escherichia coli | glutathione + NAD+ | - |
? | |
glutathione disulfide + NADH + H+ | - |
Escherichia coli S33 | glutathione + NAD+ | - |
? | |
GSSG + NADPH | - |
Escherichia coli | glutathione + NADP+ | - |
r | |
GSSG + NADPH | - |
Escherichia coli S33 | glutathione + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000 | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
highly stable, significant loss of activity only after 10 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
NADH + GSSG | Escherichia coli |
7.5 | - |
NADPH + GSSG | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
unstable below | Escherichia coli |
7.5 | 9.5 | 4°C, stable | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoprotein | Escherichia coli | |
NADPH | - |
Escherichia coli |