Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes in SK-Mel-103 melanoma cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C186A | site-directed mutagenesis, a catalytically inactive mutant, overexpression of GMPRC186A mutant at levels comparable to overexpression of wild-type GMPR does not affect invasion in of melanoma cells | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + NADPH + H+ | Homo sapiens | - |
IMP + NH3 + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P36959 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
melanocyte | - |
Homo sapiens | - |
melanoma cell | expression of guanosine monophosphate reductase (GMPR), an enzyme involved in de novo biosynthesis of purine nucleotides, is downregulated in invasive stages of human melanoma. From 9 arbitrarily chosen human metastatic melanoma cell lines, GMPR is expressed in only one cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + NADPH + H+ | - |
Homo sapiens | IMP + NH3 + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GMPR | - |
Homo sapiens |
guanosine monophosphate reductase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | expression of guanosine monophosphate reductase (GMPR), an enzyme involved in de novo biosynthesis of purine nucleotides, is downregulated in invasive stages of human melanoma | down |
General Information | Comment | Organism |
---|---|---|
malfunction | expression of guanosine monophosphate reductase (GMPR), an enzyme involved in de novo biosynthesis of purine nucleotides, is downregulated in invasive stages of human melanoma. Overexpression of catalytically inactive mutant GMPRC186A at levels comparable to overexpression of wild-type GMPR does not affect invasion in of melanoma cells | Homo sapiens |
metabolism | the enzyme activity establishes a link between guanosine metabolism and RHOGTPase-dependent melanoma cell invasion | Homo sapiens |
physiological function | GMPR downregulates the amounts of several GTP-bound (active) RHO-GTPases, and suppresses the ability of melanoma cells to form invadopodia, to degrade extracellular matrix and invade in vitro, and to grow as tumor xenografts in vivo. Enzyme GMPR partially depletes intracellular GTP pools. GMPR is a melanoma invasion suppressor, its enzymatic activity affects melanoma cell invasion and melanoma cell tumorigenicity. GMPR affects formation of invadopodia and matrix degradation. GMPR differentially regulates activity of several RHO-family GTPases, overview | Homo sapiens |