Information on EC 1.7.1.7 - GMP reductase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.7.1.7
-
RECOMMENDED NAME
GeneOntology No.
GMP reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
IMP + NH3 + NADP+ = GMP + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
reductive deamination
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Purine metabolism
-
-
purine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
inosine-5'-phosphate:NADP+ oxidoreductase (aminating)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9029-32-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mutants containing mutations in the salvage pathway of purine synthesis
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Haemophilus influenzae
-
-
-
Manually annotated by BRENDA team
no activity in Methanocaldococcus jannaschii
other name Methanococcus jannaschii
-
-
Manually annotated by BRENDA team
no activity in Mycoplasma genitalium
-
-
-
Manually annotated by BRENDA team
mRNA increases 30fold in brown adipose tissue within 6 h of cold exposure
SwissProt
Manually annotated by BRENDA team
strain B10
-
-
Manually annotated by BRENDA team
strain B10
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
NADPH + guanosine 5'-phosphate
NADP+ + inosine 5'-phosphate + NH3
show the reaction diagram
NADPH + H+ + guanosine 5'-phosphate
NADP+ + inosine 5'-phosphate + NH3
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NADPH + guanosine 5'-phosphate
NADP+ + inosine 5'-phosphate + NH3
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no activity detected with NADH or adenosine 5'-phosphate; no cofactor: NADH
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-dXMP
-
-
5,5'-dithiobis(2-nitrobenzoate)
-
0.66 mM, 33% inhibition after 30 min
6-Chloro-9-beta-D-ribofuranosylpurine 5'-phosphate
-
0.01 mM, 80% inhibition after 40 min, 1 mM GMP protect
6-Chloropurine ribonucleotide
-
-
6-Thio-GMP
6-thio-IMP
6-Thio-XMP
-
-
8-Aza-7-deaza-XMP
-
-
8-Aza-XMP
-
-
adenosine 2'-phosphate
-
-
ADP
-
2 mM, 85% inhibition
arabinosyl-XMP
-
-
Fe2+
-
10 mM, more than 90% inhibition
iodoacetamide
-
progressive inhibition, 2 mM GMP protect
iodoacetate
-
progressive inhibition, 2 mM GMP protect
Mn2+
-
-
p-hydroxymercuribenzoate
p-mercuribenzoate
-
2 mM, complete inhibition, complete protection by 2 mM GSH
XTP
-
0.05 mM, 50% inhibition
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
cysteine
-
2 mM required for maximal activity
diguanosine 5',5'''-(P1-P4-tetraphosphate)
-
-
dithiothreitol
-
activation
GSH
-
2 mM required for maximal activity
GTP
-
nonessential activator
thioglycolic acid
-
activation
additional information
-
no sulfhydryl donor required for activity
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0014 - 0.096
GMP
0.0055
guanosine 5'-phosphate
-
pH 7.8, 25C
0.0085 - 0.031
NADPH
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.28
guanosine 5'-phosphate
Escherichia coli
-
pH 7.8, 25C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
51
guanosine 5'-phosphate
Escherichia coli
-
pH 7.8, 25C
5973
19
NADPH
Escherichia coli
-
pH 7.8, 25C
5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.026
2'-dXMP
-
-
0.0003
8-Aza-XMP
-
-
0.035
IMP
-
-
0.00001 - 0.0011
XMP
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003 - 0.0076
-
activity in extracts of cells grown on different media
0.0051
-
activity in wild-type
0.0052
-
-
0.35
-
-
0.66
-
pH 7.8, 25C
0.8
-
activity in wild-type
4.6
-
activity in guaC mutant harbouring a guaC plasmid
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1
-
enzyme shows 2 maxima
7.5
-
-
7.5 - 8.2
7.8
-
enzyme shows 2 maxima, strong decrease above
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
pH 6: about 50% of activity at maximum, pH 9: about 70% of activity at maximum
7 - 9.5
-
pH 7: about 25% of activity at maximum, pH 9.5: about 30% of activity at maximum
7 - 8.2
-
strong decrease above
8.1 - 8.6
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
strong expression
Manually annotated by BRENDA team
-
expressed at low levels
Manually annotated by BRENDA team
-
expressed at low levels
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
peripheral
Manually annotated by BRENDA team
additional information
not expressed in testis, lung, liver or spleen
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
-
x * 36000, SDS-PAGE
37382
-
4 * 38000, SDS-PAGE, 4 * 37384, calculated, 4 * 37382, mass spectrometry
37383
-
4 * 38000, SDS-PAGE, 4 * 37383, calculated
37384
-
4 * 38000, SDS-PAGE, 4 * 37384, calculated, 4 * 37382, mass spectrometry
37440
-
determination of nucleotide sequence
37800
-
calculated from the deduced amino acid sequence
38000
-
4 * 38000, SDS-PAGE, 4 * 37383, calculated; 4 * 38000, SDS-PAGE, 4 * 37384, calculated, 4 * 37382, mass spectrometry
90000
-
gel filtration
156000
-
gel filtration
170000
-
gel filtration
250000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no glycoprotein
-
no glycosylation site found
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapor diffusion method
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
67
-
15 min, 40% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 50% glycerol
-
-70C, 2 months, less than 20% loss of activity
-
-70C, 2-mercaptoethanol, 6 months, less than 15% loss of activity after several rounds of freezing and thawing
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-80C, no loss of activity
-
-80C, stable
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity purification on Cibacron blue
-
from recombinant COS-7 cells
-
recombinant enzyme using His-tag
-
recombinant His-tagged enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli M15 as His-tag fusion protein
-
expression in Escherichia coli
in vitro transcription/translation
-
transfection of HL-60 leukemia cells, expressed in COS-7 cells as His-tag fusion protein
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
potential target for the treatment of leukemia
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