Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no sulfhydryl donor required for activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 49% inhibition | Homo sapiens | |
Cu2+ | 1 mM, more than 90% inhibition | Homo sapiens | |
GTP | 0.05 mM, 30% inhibition | Homo sapiens | |
Mg2+ | 1 mM, 9% inhibition | Homo sapiens | |
additional information | not inhibited by ATP | Homo sapiens | |
Ni2+ | 1 mM, more than 90% inhibition | Homo sapiens | |
XMP | 0.05 mM, 99% inhibition | Homo sapiens | |
XTP | 0.05 mM, 50% inhibition | Homo sapiens | |
Zn2+ | 1 mM, 86% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0174 | - |
GMP | - |
Homo sapiens | |
0.0266 | - |
NADPH | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, recombinant His-tagged enzyme, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q9P2T1 | - |
- |
no activity in Haemophilus influenzae | - |
- |
- |
no activity in Methanocaldococcus jannaschii | - |
other name Methanococcus jannaschii | - |
no activity in Mycoplasma genitalium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | strong expression | Homo sapiens | - |
cardiac muscle | - |
Homo sapiens | - |
colon | expressed at low levels | Homo sapiens | - |
kidney | - |
Homo sapiens | - |
leukocyte | expressed at low levels | Homo sapiens | - |
liver | strong expression | Homo sapiens | - |
placenta | strong expression | Homo sapiens | - |
skeletal muscle | - |
Homo sapiens | - |
thymus | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + guanosine 5'-phosphate | - |
Homo sapiens | NADP+ + inosine 5'-phosphate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, recombinant His-tagged enzyme, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
enzyme shows 2 maxima | Homo sapiens |
7.8 | - |
enzyme shows 2 maxima, strong decrease above | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.2 | strong decrease above | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens | |
NADPH | no activity with NADH | Homo sapiens |