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Literature summary for 1.7.1.4 extracted from
- Purification and properties of the Neurospora crassa assimilatory nitrite reductase (1974), J. Biol. Chem., 249, 7555-7567.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Neurospora crassa |  |
| CN- | - |
Neurospora crassa | |
| hydroxylamine | - |
Neurospora crassa | |
| KCN | - |
Neurospora crassa | |
| Na2S | - |
Neurospora crassa | |
| NAD(P)H | - |
Neurospora crassa | |
| p-hydroxymercuribenzoate | very potent inhibitor, inhibition can be reversed by either cysteine or glutathione | Neurospora crassa | |
| sodium disulfite | - |
Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
FAD | - |
Neurospora crassa | |
| 0.01 | - |
NO2- | cosubstrate NADPH or NADH | Neurospora crassa | |
| 0.8 | - |
NO2- | + dithionite | Neurospora crassa | |
| 2.5 | - |
hydroxylamine | - |
Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 290000 | - |
gel filtration, sucrose density gradient centrifugation | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD(P)H + nitrite + H+ | Neurospora crassa | - |
NAD(P)+ + NH3 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.52 | - |
NADH + NO2- | Neurospora crassa |
| 0.73 | - |
NADPH + NO2- | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD(P)H + NH2OH | - |
Neurospora crassa | NAD(P)+ + NH3 + H2O | - |
? | |
| NAD(P)H + nitrite + H+ | - |
Neurospora crassa | NAD(P)+ + NH3 + H2O | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
80% activity is lost after 4 min | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Neurospora crassa | |
| NADPH | - |
Neurospora crassa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
p-hydroxymercuribenzoate | - |
Neurospora crassa | |
| 0.005 | - |
KCN | - |
Neurospora crassa | |
| 2 | - |
1,10-phenanthroline | - |
Neurospora crassa | |
| 2 | - |
sodium disulfite | - |
Neurospora crassa | |
| 5 | - |
Sodium sulfide | - |
Neurospora crassa | |
| 6 | - |
hydroxylamine | - |
Neurospora crassa | |
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