Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H109R | natural polymorphism identified in a dimethylglycine dehydrogenase-deficient patient. Mutant shows only 47% of the wild-type level of bound flavin, a 27fold decrease in specific activity and a 65fold increase in Km value | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
N,N-dimethylglycine | and second value 15.4 mM, wild-type, pH 7.5, 25°C | Homo sapiens | |
0.045 | - |
N,N-dimethylglycine | and second value 1.10 mM, wild-type, presence of tetrahydrofolate, pH 7.5, 25°C | Homo sapiens | |
1.1 | - |
N,N-dimethylglycine | and first value 0.045 mM, wild-type, presence of tetrahydrofolate, pH 7.5, 25°C | Homo sapiens | |
2.5 | 5 | N,N-dimethylglycine | mutant H109R, pH 7.5, 25°C | Homo sapiens | |
15.4 | - |
N,N-dimethylglycine | and first value 0.039 mM, wild-type, pH 7.5, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0068 | - |
mutant H109R, pH 7.5, 25°C | Homo sapiens |
0.165 | - |
wild-type, pH 7.5, 25°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethylglycine + ferricenium hexafluorophosphate + H2O | - |
Homo sapiens | sarcosine + formaldehyde + reduced ferricenium hexafluorophosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DMGDH | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
N,N-dimethylglycine | mutant H109R, pH 7.5, 25°C | Homo sapiens | |
0.225 | - |
N,N-dimethylglycine | wild-type, presence of tetrahydrofolate, pH 7.5, 25°C | Homo sapiens | |
0.3 | - |
N,N-dimethylglycine | wild-type, pH 7.5, 25°C | Homo sapiens |