Information on EC 1.5.8.4 - dimethylglycine dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.5.8.4
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RECOMMENDED NAME
GeneOntology No.
dimethylglycine dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N,N-dimethylglycine + electron-transfer flavoprotein + H2O = sarcosine + formaldehyde + reduced electron-transfer flavoprotein
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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oxidative deamination
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycine betaine degradation I
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Glycine, serine and threonine metabolism
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Metabolic pathways
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creatinine degradation
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SYSTEMATIC NAME
IUBMB Comments
N,N-dimethylglycine:electron-transfer flavoprotein oxidoreductase (demethylating)
A flavoprotein, containing a histidyl(N3)-(8alpha)FAD linkage
CAS REGISTRY NUMBER
COMMENTARY hide
37256-30-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
monkey
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,10-methenyltetrahydrofolate + FADH2
5,10-methylene tetrahydrofolate + FAD
show the reaction diagram
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-
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epsilon-N-methyl-L-lysine + acceptor + H2O
formaldehyde + L-lysine + reduced acceptor
show the reaction diagram
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-
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
show the reaction diagram
N,N-dimethylglycine + ferricenium hexafluorophosphate + H2O
sarcosine + formaldehyde + reduced ferricenium hexafluorophosphate
show the reaction diagram
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-
-
-
?
N,N-dimethylglycine + H2O + FAD
sarcosine + formaldehyde + FADH2
show the reaction diagram
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N,N-dimethylglycine + H2O + oxidized 1-methoxy-5-methylphenazinium methylsulfate
sarcosine + formaldehyde + reduced 1-methoxy-5-methylphenazinium methylsulfate
show the reaction diagram
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-
-
-
?
N,N-dimethylglycine + H2O + oxidized 2,6-dichlorophenolindophenol
sarcosine + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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-
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?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
show the reaction diagram
N-methyl-L-alanine + acceptor + H2O
formaldehyde + L-alanine + reduced acceptor
show the reaction diagram
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-
?
sarcosine + acceptor + H2O
glycine + formaldehyde + reduced acceptor
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5,10-methenyltetrahydrofolate + FADH2
5,10-methylene tetrahydrofolate + FAD
show the reaction diagram
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N,N-dimethylglycine + H2O + FAD
sarcosine + formaldehyde + FADH2
show the reaction diagram
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N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Tetrahydropteroylpentaglutamate
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additional information
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1-methoxy-5-methylphenazinium methylsulfate can act as electron acceptor; 2,6-dichlorophenolindophenol can act as electron acceptor
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Nonheme iron
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Dimethylthetin
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Methoxyacetate
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competitive inhibitor at low substrate concentrations
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
dimethylglycine
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0.039 - 15.4
N,N-dimethylglycine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01 - 0.3
N,N-dimethylglycine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0068
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mutant H109R, pH 7.5, 25°C
0.157 - 0.268
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0.165
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wild-type, pH 7.5, 25°C
0.24
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purified recombinant enzyme from soluble fraction
129
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potassium phosphate fraction
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 9
8.5
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recombinant and native enzyme
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
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recombinant and native enzyme
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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detectable levels of expression demonstrated at the RNA and protein level
Manually annotated by BRENDA team
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detectable levels of expression demonstrated at the RNA and protein level
Manually annotated by BRENDA team
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predominant expression of the enzyme
Manually annotated by BRENDA team
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detectable levels of expression demonstrated at the RNA and protein level
Manually annotated by BRENDA team
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detectable levels of expression demonstrated at the RNA and protein level
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
94000
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His-tagged pMe2GlyDH, SDS-PAGE
97410
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pMe2GlyDH, calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-4°C, lyophilized, 1 week
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme from liver mitochondria, recombinant His-tagged enzyme from Escherichia coli 45fold by nickel affinity chromatography
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Ni2+-Sepharose column chromatography
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of in vitro synthesized 6-His-dimethylglycine dehydrogenase on nickel chelating Sepharose
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using ammonium sulfate treatment and dissolution in 7.5 mM potassium phosphate
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using gel filtration chromatography on Sephadex G-150, DEAE-cellulose chromatography, and affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells as a C-terminally 6-His-tagged fusion protein which is predominantly in its apo form
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expression in Escherichia coli
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overexpression in Escherichia coli strain JM109 as N-terminally His-tagged enzyme being equally distributed in the soluble fraction and the insoluble inclusion body fraction
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screening of a cDNA library prepared in Escherichia coli from rat liver polyA RNA in the plasmid vector peD-X
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H109R
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natural polymorphism identified in a dimethylglycine dehydrogenase-deficient patient. Mutant shows only 47% of the wild-type level of bound flavin, a 27fold decrease in specific activity and a 65fold increase in Km value
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
refolding by 80fold dilution after solubilization from inclusion bodies by urea treatment, 50-60% reactivation yield, overview
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