Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-diaminopropane | 10 mM with 2 mM N-methylputrescine as substrate, 32% relative inhibition | Hyoscyamus niger | |
1,3-diaminopropane | 10 mM with 2 mM N-methylputrescine as substrate, 70% relative inhibition | Pisum sativum | |
1,3-diaminopropane | 10 mM with 2 mM N-methylputrescine as substrate, 93% relative inhibition | Sus scrofa | |
aminoguanidine | 66% inhibition at 10 mM | Hyoscyamus niger | |
CaCl2 | 1 mM, slightly inhibited,less than 24% | Hyoscyamus niger | |
CdCl2 | 1 mM, strongly inhibited,more than 90% | Hyoscyamus niger | |
CoCl2 | 1 mM, moderately inhibited,60-80% | Hyoscyamus niger | |
CuCl2 | 1 mM, strongly inhibited,more than 90% | Hyoscyamus niger | |
diethyldithiocarbamate | 59% inhibition at 0.1 mM | Hyoscyamus niger | |
FeSO4 | 1 mM, moderately inhibited,60-80% | Hyoscyamus niger | |
HgCl2 | 1 mM, strongly inhibited,more than 90% | Hyoscyamus niger | |
hydroxylamine | 40% inhibition at 0.01 mM | Hyoscyamus niger | |
KCN | 1 mM, inhibits activity completely, is used to stop the enzyme reaction | Hyoscyamus niger | |
MgCl2 | 1 mM, slightly inhibited,less than 24% | Hyoscyamus niger | |
MnCl2 | 1 mM, slightly inhibited,less than 24% | Hyoscyamus niger | |
additional information | inhibition by the copper ligand diethyldithiocarbamate and the carbonyl reagents suggests that copper and pyrrroloquinoline are present at the active site of this enzyme; primary amines inhibit pea and pig kidney DAO activities more strongly than the Hyoscyamus niger. N-methylated amines are stronger inhibitors of the Hyoscyamus enzyme than of the others DAOs | Hyoscyamus niger | |
additional information | primary amines inhibit pea and pig kidney DAO activities more strongly than the Hyoscyamus niger. N-methylated amines are stronger inhibitors of the Hyoscyamus enzyme than of the others DAOs | Pisum sativum | |
additional information | primary amines inhibit pea and pig kidney DAO activities more strongly than the Hyoscyamus niger. N-methylated amines are stronger inhibitors of the Hyoscyamus enzyme than of the others DAOs | Sus scrofa | |
n-butylamine | 10 mM with 2 mM N-methylputrescine as substrate, 19% relative inhibition | Hyoscyamus niger | |
n-butylamine | 10 mM with 2 mM N-methylputrescine as substrate, 66% relative inhibition | Pisum sativum | |
n-butylamine | 10 mM with 2 mM N-methylputrescine as substrate, 60% relative inhibition | Sus scrofa | |
N-methyl-1,3-diamonopropane | 10 mM with 2 mM N-methylputrescine as substrate, 34% relative inhibition | Hyoscyamus niger | |
N-methyl-1,3-diamonopropane | 10 mM with 2 mM N-methylputrescine as substrate, 55% relative inhibition | Pisum sativum | |
N-methyl-1,3-diamonopropane | 10 mM with 2 mM N-methylputrescine as substrate, 61% relative inhibition | Sus scrofa | |
N-methylbutylamine | 10 mM with 2 mM N-methylputrescine as substrate, 70% relative inhibition | Hyoscyamus niger | |
N-methylbutylamine | 10 mM with 2 mM N-methylputrescine as substrate, 23% relative inhibition | Pisum sativum | |
N-methylbutylamine | 10 mM with 2 mM N-methylputrescine as substrate, 19% relative inhibition | Sus scrofa | |
N-Methylmaleimide | 59% inhibition at 1 mM | Hyoscyamus niger | |
N-methylpropylamine | 10 mM with 2 mM N-methylputrescine as substrate, 75% relative inhibition | Hyoscyamus niger | |
N-methylpropylamine | 10 mM with 2 mM N-methylputrescine as substrate, 13% relative inhibition | Pisum sativum | |
N-methylpropylamine | 10 mM with 2 mM N-methylputrescine as substrate, 22% relative inhibition | Sus scrofa | |
n-Propylamine | 10 mM with 2 mM N-methylputrescine as substrate, 28% relative inhibition | Hyoscyamus niger | |
n-Propylamine | 10 mM with 2 mM N-methylputrescine as substrate, 23% relative inhibition | Pisum sativum | |
n-Propylamine | 10 mM with 2 mM N-methylputrescine as substrate, 48% relative inhibition | Sus scrofa | |
NiCl2 | 1 mM, moderately inhibited,60-80% | Hyoscyamus niger | |
p-chloromercuribenzenesulfonic acid | 91% inhibition at 1 mM | Hyoscyamus niger | |
phenylhydrazine | 69% inhibition at 0.01 mM | Hyoscyamus niger | |
Semicarbazide | 57% inhibition at 10 mM | Hyoscyamus niger | |
ZnCl2 | 1 mM, slightly inhibited,less than 24% | Hyoscyamus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
cadaverine | pH 7.9, 30°C, lowest KM-value compared with N-putrescine or cadaverine as substrates, Vmax: 96.2 pkat | Pisum sativum | |
0.3 | - |
cadaverine | pH 7.9, 30°C, higher KM-value compared with N-putrescine or cadaverine as substrates, Vmax: 20.5 pkat | Sus scrofa | |
0.31 | - |
1,4-diaminobutane | pH 7.9, 30°C, higher KM-value compared with N-methylputrescine or cadaverine as substrates, Vmax: 21.9 pkat | Sus scrofa | |
0.32 | - |
1,4-diaminobutane | pH 7.9, 30°C, lower KM-value compared with N-methylputrescine and higher compared to cadaverine as substrates, Vmax: 97.3 pkat | Pisum sativum | |
0.33 | - |
N-methylputrescine | pH 7.9, 30°C, lowest KM-value compared with putrescine or cadaverine as substrates, Vmax: 90.4 pkat | Hyoscyamus niger | |
0.82 | - |
N-methylputrescine | pH 7.9, 30°C, lowest KM-value compared with putrescine or cadaverine as substrates, Vmax: 23.3 pkat | Sus scrofa | |
1.22 | - |
N-methylputrescine | pH 7.9, 30°C, highest KM-value compared with putrescine or cadaverine as substrates, Vmax: 93.5 pkat | Pisum sativum | |
2.85 | - |
1,4-diaminobutane | pH 7.9, 30°C, higher KM-value compared with N-methylputrescine or cadaverine as substrates, Vmax: 82.7 pkat, specifiity constant 11% of the constant for N-methylputrescine | Hyoscyamus niger | |
6.25 | - |
cadaverine | pH 7.9, 30°C, higher KM-value compared with N-putrescine or cadaverine as substrates, Vmax: 19.6 pkat, specifiity constant 1% of the constant for N-methylputrescine | Hyoscyamus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
2 * 66000, SDS-PAGE | Hyoscyamus niger |
135000 | - |
gel filtration | Hyoscyamus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyoscyamus niger | - |
- |
- |
Pisum sativum | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially | Hyoscyamus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epicotyl | - |
Pisum sativum | - |
kidney | - |
Sus scrofa | - |
root culture | - |
Hyoscyamus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0326 | - |
partially purified enzyme, N-methylpurtrescine as substrate, pH 7.9, 30°C | Hyoscyamus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-diaminopropane + H2O + O2 | 30% activity compared to activity with N-methylputrescine | Hyoscyamus niger | 3-aminopropanal + NH3 + H2O2 | - |
? | |
1,3-diaminopropane + H2O + O2 | 30% activity compared to activity with putrescine | Sus scrofa | 3-aminopropanal + NH3 + H2O2 | - |
? | |
1,4-diaminobutane + H2O + O2 | best substrate | Sus scrofa | 4-aminobutanal + NH3 + H2O2 | - |
? | |
1,4-diaminobutane + H2O + O2 | best substrate | Pisum sativum | 4-aminobutanal + NH3 + H2O2 | - |
? | |
1,4-diaminobutane + H2O + O2 | 50% activity compared to activity with N-methylputrescine | Hyoscyamus niger | 4-aminobutanal + NH3 + H2O2 | - |
? | |
cadaverine + H2O + O2 | 30% activity compared to activity with N-methylputrescine | Hyoscyamus niger | 5-aminopentanal + NH3 + H2O2 | - |
? | |
cadaverine + H2O + O2 | 75% activity compared to activity with putrescine | Pisum sativum | 5-aminopentanal + NH3 + H2O2 | - |
? | |
cadaverine + H2O + O2 | 80% activity compared to activity with putrescine | Sus scrofa | 5-aminopentanal + NH3 + H2O2 | - |
? | |
histamine + H2O + O2 | 60% activity compared to activity with putrescine | Sus scrofa | (imidazol-4-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
additional information | low activity with histamine, n-butylamine, and 1,6-diaminohexane | Hyoscyamus niger | ? | - |
? | |
additional information | low activity with n-butylamine or 1,6-diaminohexane as substrate | Sus scrofa | ? | - |
? | |
additional information | low or no activity with 1,3-diaminopropane, N-methyl-1,3-diaminopropane, n-butylamine, 1,6-diaminohexane, and histamine | Pisum sativum | ? | - |
? | |
N-methyl-1,3-diaminopropane + H2O + O2 | 40% activity compared to activity with N-methylputrescine | Hyoscyamus niger | N-methyl-3-aminopropanal + NH3 + H2O2 | - |
? | |
N-methyl-1,3-diaminopropane + H2O + O2 | 40% activity compared to activity with putrescine | Sus scrofa | N-methyl-3-aminopropanal + NH3 + H2O2 | - |
? | |
N-methylputrescine + H2O + O2 | 50% activity compared to activity with putrescine | Pisum sativum | 4-(methylamino)butanal + NH3 + H2O2 | - |
? | |
N-methylputrescine + H2O + O2 | 75% activity compared to activity with putrescine | Sus scrofa | 4-(methylamino)butanal + NH3 + H2O2 | - |
? | |
N-methylputrescine + H2O + O2 | best substrate. Specificity for N-methylated diamines enable the Hyoscyamine enzyme to function specifically in tropane alkaloid biosynthesis | Hyoscyamus niger | 4-(methylamino)butanal + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 66000, SDS-PAGE | Hyoscyamus niger |
Synonyms | Comment | Organism |
---|---|---|
DAO | - |
Hyoscyamus niger |
EC 1.4.3.6 | formerly | Hyoscyamus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hyoscyamus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | measured over the range of 6.0 to 9.0, with N-methylputrescine as substrate | Hyoscyamus niger |
7.9 | - |
assay at | Hyoscyamus niger |
General Information | Comment | Organism |
---|---|---|
physiological function | root cultures of Hyoscyamus niger produce nicotine and tropane alkaloids by the same early biosynthetic pathway in which the DAO functions to convert N-methylputrescine to methylpyrrolinium cation. There is no close correlaion between alkaloid production and DAO activity. The biosynthesis of nicotine and tropane alkaloids are not regulated directly by the DAO activities. Specificty for N-methylated diamines enable the Hyoscyamine enzyme to function specifically in tropane alkaloid biosynthesis | Hyoscyamus niger |