Protein Variants | Comment | Organism |
---|---|---|
H124A | inactive | Glycine max |
H124N | substantial decrease in catalytic efficiency | Glycine max |
H124Q | inactive | Glycine max |
H188A | inactive | Glycine max |
H188N | inactive | Glycine max |
H188Q | inactive | Glycine max |
N222A | complete loss of activity | Glycine max |
N222Q | 30fold decrease in kcat value and a modest change in Km value | Glycine max |
Q130A | 300fold lower turnover rate | Glycine max |
Q130D | 4fold reduction in kcat values and less than twofold increase in Km value | Glycine max |
Q130E | 4fold reduction in kcat values and less than twofold increase in Km value | Glycine max |
Q130S | 35fold lower turnover rate | Glycine max |
Q184A | 320fold lower kcat value, with a Km value comparable to that of the wild-type | Glycine max |
Q184D | loss of detectable PDH activity | Glycine max |
Q184E | loss of detectable PDH activity | Glycine max |
S101A | mutantion primarily affectes the Km value for prephenate with a 20fold increase | Glycine max |
T131A | 134fold decrease in kcat value | Glycine max |
T131S | 272fold decrease in kcat value | Glycine max |
T206A | loss of detectable activity | Glycine max |
T206S | tremendous loss of activity | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
prephenate | mutant T206S, pH 7.5, 37°C | Glycine max | |
0.17 | - |
prephenate | mutant T131S, pH 7.5, 37°C | Glycine max | |
0.21 | - |
prephenate | mutant T131A, pH 7.5, 37°C | Glycine max | |
0.22 | - |
prephenate | mutant Q130A, pH 7.5, 37°C | Glycine max | |
0.26 | - |
prephenate | mutant Q130S, pH 7.5, 37°C | Glycine max | |
0.27 | - |
prephenate | wild-type, pH 7.5, 37°C | Glycine max | |
0.29 | - |
prephenate | mutant H124N, pH 7.5, 37°C | Glycine max | |
0.37 | - |
prephenate | mutant Q184A, pH 7.5, 37°C | Glycine max | |
0.44 | - |
prephenate | mutant Q130E, pH 7.5, 37°C | Glycine max | |
0.48 | - |
prephenate | mutant Q130D, pH 7.5, 37°C | Glycine max | |
0.49 | - |
prephenate | mutant N222Q, pH 7.5, 37°C | Glycine max | |
5.2 | - |
prephenate | mutant S101A, pH 7.5, 37°C | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NADP+ | - |
Glycine max | 4-hydroxyphenylpyruvate + CO2 + NADPH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
prephenate | mutant T206S, pH 7.5, 37°C | Glycine max | |
0.052 | - |
prephenate | mutant Q184A, pH 7.5, 37°C | Glycine max | |
0.055 | - |
prephenate | mutant Q130A, pH 7.5, 37°C | Glycine max | |
0.06 | - |
prephenate | mutant T131S, pH 7.5, 37°C | Glycine max | |
0.075 | - |
prephenate | mutant H124N, pH 7.5, 37°C | Glycine max | |
0.122 | - |
prephenate | mutant T131A, pH 7.5, 37°C | Glycine max | |
0.5 | - |
prephenate | mutant N222Q, pH 7.5, 37°C | Glycine max | |
0.583 | - |
prephenate | mutant Q130S, pH 7.5, 37°C | Glycine max | |
2.83 | - |
prephenate | mutant Q130D, pH 7.5, 37°C | Glycine max | |
3.83 | - |
prephenate | mutant Q130E, pH 7.5, 37°C | Glycine max | |
16.3 | - |
prephenate | wild-type, pH 7.5, 37°C | Glycine max | |
18.3 | - |
prephenate | mutant S101A, pH 7.5, 37°C | Glycine max |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
prephenate | mutant H124N, pH 7.5, 37°C | Glycine max | |
0.13 | - |
prephenate | mutant T206S, pH 7.5, 37°C | Glycine max | |
0.14 | - |
prephenate | mutant Q184A, pH 7.5, 37°C | Glycine max | |
0.25 | - |
prephenate | mutant Q130A, pH 7.5, 37°C | Glycine max | |
0.36 | - |
prephenate | mutant T131S, pH 7.5, 37°C | Glycine max | |
0.58 | - |
prephenate | mutant T131A, pH 7.5, 37°C | Glycine max | |
1.02 | - |
prephenate | mutant N222Q, pH 7.5, 37°C | Glycine max | |
2.22 | - |
prephenate | mutant Q130S, pH 7.5, 37°C | Glycine max | |
3.53 | - |
prephenate | mutant S101A, pH 7.5, 37°C | Glycine max | |
5.9 | - |
prephenate | mutant Q130D, pH 7.5, 37°C | Glycine max | |
8.71 | - |
prephenate | mutant Q130E, pH 7.5, 37°C | Glycine max | |
60.5 | - |
prephenate | wild-type, pH 7.5, 37°C | Glycine max |