Information on EC 1.3.1.13 - prephenate dehydrogenase (NADP+)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.3.1.13
-
RECOMMENDED NAME
GeneOntology No.
prephenate dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
prephenate + NADP+ = 4-hydroxyphenylpyruvate + CO2 + NADPH
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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oxidative decarboxylation
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-
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redox reaction
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-
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Phenylalanine, tyrosine and tryptophan biosynthesis
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Metabolic pathways
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Biosynthesis of secondary metabolites
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
prephenate:NADP+ oxidoreductase (decarboxylating)
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CAS REGISTRY NUMBER
COMMENTARY hide
37251-11-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ATCC 14987; ATCC 23055
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Manually annotated by BRENDA team
ATCC 15309
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Manually annotated by BRENDA team
strain C1W
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Manually annotated by BRENDA team
strain C1W
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Manually annotated by BRENDA team
strain ATCC 27893, 27898, 29151 and 29413
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Manually annotated by BRENDA team
strain ATCC 29538
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Lyngbya sp.
strain ATCC 29119, 29121 and 29126
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Manually annotated by BRENDA team
no activity in Anabaena sp.
strains ATCC 27892, 27899, 29211 and 29414
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-
Manually annotated by BRENDA team
no activity in Arabidopsis thaliana
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Manually annotated by BRENDA team
no activity in Beta vulgaris subsp. vulgaris
red beet, Beta vulgaris ssp. var. conditiva, Beta vulgaris ssp. vulgaris var. rubra
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-
Manually annotated by BRENDA team
no activity in Calothrix sp.
strain ATCC 27901, 27905, 29112, 29156, 29158and 29171
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Manually annotated by BRENDA team
no activity in Nostoc sp.
strain ATCC 29411 and 29132
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-
Manually annotated by BRENDA team
no activity in Oryza sativa
rice
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Manually annotated by BRENDA team
no activity in Pisum sativum
pea
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Manually annotated by BRENDA team
no activity in Scytonema sp.
strain 29171
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-
Manually annotated by BRENDA team
no activity in Scytonema sp. 29171
strain 29171
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Manually annotated by BRENDA team
no activity in Synechocystis sp.
strain ATCC 29108
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Manually annotated by BRENDA team
no activity in Triticum aestivum
wheat
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Manually annotated by BRENDA team
strain ATCC 29105 and 29133
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Manually annotated by BRENDA team
scarlet runner bean, L.
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Manually annotated by BRENDA team
wax bean, L. var.Bountiful
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Manually annotated by BRENDA team
Pseudomonas alkanolytica
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Manually annotated by BRENDA team
strain ATCC 29404, Agmenellum quadruplicatum BG1, strain 27144, 27146 and 27192
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Manually annotated by BRENDA team
strain ATCC 29109,27178 and 29235
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Manually annotated by BRENDA team
broad bean, L. var. Windsor
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Manually annotated by BRENDA team
mung bean, [L.] Wilczek, originally classified as Phaseolus aureus [L.] Roxb.
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
prephenate + NAD+
4-hydroxyphenylpyruvate + CO2 + NADH
show the reaction diagram
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
prephenate + NAD+
4-hydroxyphenylpyruvate + CO2 + NADH
show the reaction diagram
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuriphenylsulfonic acid
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L-tyrosine
N-ethylmaleimide
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p-hydroxymercurybenzoic acid
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additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.561 - 3.3
NAD+
0.005 - 0.36
NADP+
0.033 - 0.73
prephenate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15 - 26.3
prephenate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
47 - 217
prephenate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.009
L-tyrosine
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competitive inhibition with respect to substrate and noncompetitively with respect to cofactor
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10.8
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000
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gel filtration
71000
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analytical ultracentrifugation
85000
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analytical ultracentrifugation
210000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 40612, calculated from amino acid sequence
homodimer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
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maximum stability in this range at 22°C
390556
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54
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melting temperature
55
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up to 10 min inactivates the enzyme
80 - 95
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
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Ni-NTA column chromatography, and Superdex 200 gel filtration
partially
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strain 23055, partially
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells