Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. The OcAOD productivity of the recombinant E. coli is 24fold higher than that of Ochrobactrum sp. AIU 033, and it is further enhanced by 1.2 times by the coexpression of additional tatABC from E. coli BL21 | Ochrobactrum sp. AIU 033 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | recombinant protein in Escherichia coli | Ochrobactrum sp. AIU 033 | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ochrobactrum sp. AIU 033 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + O2 | - |
Ochrobactrum sp. AIU 033 | butanal + H2O2 | - |
? | |
1-hexanol + O2 | - |
Ochrobactrum sp. AIU 033 | hexanal + H2O2 | - |
? | |
1-octanol + O2 | - |
Ochrobactrum sp. AIU 033 | octanal + H2O2 | - |
? | |
1-pentanol + O2 | - |
Ochrobactrum sp. AIU 033 | pentanal + H2O2 | - |
? | |
1-propanol + O2 | - |
Ochrobactrum sp. AIU 033 | propanal + H2O2 | - |
? | |
ethanol + O2 | - |
Ochrobactrum sp. AIU 033 | acetaldehyde + H2O2 | - |
? | |
additional information | enzyme also catalyzes the oxidation of glycolate to glyoxylate, reaction of EC 1.1.3.15. No substrate: glyoxylate | Ochrobactrum sp. AIU 033 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, alpha-subunit, x * 14000, beta-subunit, SDS-PAGE | Ochrobactrum sp. AIU 033 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
20 min, more than 80% residual activity, recombinant protein | Ochrobactrum sp. AIU 033 |
50 | - |
20 min, more than 40% residual activity for recombinant protein, complete loss of activity for native protein | Ochrobactrum sp. AIU 033 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | 20 min, 25°C, more than 80% residual activity, recombinant protein | Ochrobactrum sp. AIU 033 |