Information on EC 1.1.3.13 - alcohol oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.3.13
-
RECOMMENDED NAME
GeneOntology No.
alcohol oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a primary alcohol + O2 = an aldehyde + H2O2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
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redox reaction
-
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reduction
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
methanol oxidation to formaldehyde IV
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methane metabolism
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Methane metabolism
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-
Metabolic pathways
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Microbial metabolism in diverse environments
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-
SYSTEMATIC NAME
IUBMB Comments
alcohol:oxygen oxidoreductase
The enzymes from the fungi Candida methanosorbosa and several Basidiomycetes species contain an FAD cofactor [1,3]. The enzyme from the phytopathogenic fungi Colletotrichum graminicola and Colletotrichum gloeosporioides utilize a mononuclear copper-radical mechanism [4]. The enzyme acts on primary alcohols and unsaturated alcohols, and has much lower activity with branched-chain and secondary alcohols.
CAS REGISTRY NUMBER
COMMENTARY hide
9073-63-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
isolated as a formaldehyde-resistant fungus from wastewater containing formaldehyde
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Manually annotated by BRENDA team
isolated as a formaldehyde-resistant fungus from wastewater containing formaldehyde
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Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
isolated in Thailand
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-
Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
gene faot
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
indigenous, filamentous fungus isolated from petroleum-contaminated soils,growth on decane or hexadecane, two isoforms
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
Hansenula sp.
-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
fragment; var. minuta
SwissProt
Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
gene AOX1
-
-
Manually annotated by BRENDA team
isolated in Thailand
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-
Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
gene AOX1
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-
Manually annotated by BRENDA team
gene AOX
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-
Manually annotated by BRENDA team
Pichia putida
-
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Manually annotated by BRENDA team
Polyporus obtusus
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Manually annotated by BRENDA team
Poria contigua
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Manually annotated by BRENDA team
thermophilic fungus
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Manually annotated by BRENDA team
thermophilic fungus
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the peroxisomal enzyme alcohol oxidase plays key role in methanol dissimilation in yeast
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-2-octanol + O2
2-octanone + H2O2
show the reaction diagram
-
-
-
-
?
(R)-2-octanol + O2
octan-2-one + H2O2
show the reaction diagram
1,2-ethanediol + O2
?
show the reaction diagram
-
-
-
-
?
1,2-propanediol + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
1,3-butylene glycol + O2
? + H2O2
show the reaction diagram
1,4-butynediol + O2
4-hydroxy-2-butyn-1-al + H2O2
show the reaction diagram
-
oxidized to mechanism-based irreversible inactivator: 4-hydroxy-2-butynal
mechanism-based inhibitor
?
1-butanol + O2
butanal + H2O2
show the reaction diagram
1-butanol + O2
butanaldehyde + H2O2
show the reaction diagram
-
intra- and extracellular enzymes, low activity with the intracellular enzyme
-
-
?
1-butanol + O2
n-butanal + H2O2
show the reaction diagram
1-decanol + O2
decanal + H2O2
show the reaction diagram
-
180% activity compared to glycolate
-
-
?
1-hexanol + O2
hexanal + H2O2
show the reaction diagram
-
300% activity compared to glycolate
-
-
?
1-octanol + O2
n-octanal + H2O2
show the reaction diagram
1-octanol + O2
octanal + H2O2
show the reaction diagram
-
189% activity compared to glycolate
-
-
?
1-pentanol + O2
n-pentanal + H2O2
show the reaction diagram
1-pentanol + O2
pentanal + H2O2
show the reaction diagram
-
448% activity compared to glycolate
-
-
?
1-pentanol + O2
pentanaldehyde + H2O2
show the reaction diagram
-
intra- and extracellular enzymes, low activity with the intracellular enzyme
-
-
?
1-propanol + O2
n-propanal + H2O2
show the reaction diagram
1-propanol + O2
propanal + H2O2
show the reaction diagram
1-undecanol + O2
undecanal + H2O2
show the reaction diagram
2-butanol + O2
2-butanone + H2O2
show the reaction diagram
2-buten-1-ol + O2
2-buten-1-al + H2O2
show the reaction diagram
-
-
-
-
?
2-chloroethanol + O2
2-chloroethanal + H2O2
show the reaction diagram
-
66% of the activity with methanol
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?
2-cyanoethanol + O2
2-cyanoethanal + H2O2
show the reaction diagram
-
30% of the activity with methanol
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-
?
2-dodecanol + O2
dodecan-2-one + H2O2
show the reaction diagram
2-mercaptoethanol + O2
2-mercaptoethanal + H2O2
show the reaction diagram
2-methoxyethanol + O2
2-methoxyethanal + H2O2
show the reaction diagram
-
40% of the activity with methanol
-
-
?
2-methyl-1-butanol
2-methylbutanal + H2O2
show the reaction diagram
-
-
-
-
?
2-methyl-1-butanol + O2
2-methyl-1-butanal + H2O2
show the reaction diagram
-
22% of the activity with methanol
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-
?
2-methyl-1-hexanol
2-methylhexanal + H2O2
show the reaction diagram
-
-
-
-
?
2-methyl-1-pentanol
2-methylpentanal + H2O2
show the reaction diagram
-
-
-
-
?
2-methyl-1-propanol + O2
butan-2-one + H2O2
show the reaction diagram
7% of the activity with methanol
-
-
?
2-methyl-2-propanol + O2
?
show the reaction diagram
-
extracellular enzyme, no activity with the intracellular enzyme
-
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?
2-propanol + O2
2-oxopropane + H2O2
show the reaction diagram
-
-
-
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?
2-propanol + O2
acetone + H2O2
show the reaction diagram
2-propen-1-ol + O2
2-propen-1-al + H2O2
show the reaction diagram
2-propyn-1-ol + O2
2-propyn-1-al + H2O2
show the reaction diagram
Poria contigua
-
-
-
-
?
2-propyn-1-ol + O2
propyn-1-al + H2O2
show the reaction diagram
3-buten-1-ol + O2
but-3-enal + H2O2
show the reaction diagram
-
-
-
-
?
3-chloro-1-propanol + O2
3-chloro-1-propanal + H2O2
show the reaction diagram
-
22% of the activity with methanol
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?
3-methyl-1-butanol + O2
3-methylbutanal + H2O2
show the reaction diagram
7% of the activity with methanol
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?
3-phenylpropan-1-ol + O2
3-phenylpropanal + H2O2
show the reaction diagram
4-chloro-1-butanol + O2
4-chloro-1-butanal + H2O2
show the reaction diagram
-
11% of the activity with methanol
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-
?
4-hepten-1-ol + O2
4-hepten-1-al + H2O
show the reaction diagram
-
-
-
?
4-nonen-1-ol + O2
4-nonen-1-al + H2O2
show the reaction diagram
-
-
-
?
4-penten-1-ol + O2
pent-4-enal + H2o2
show the reaction diagram
-
-
-
-
?
allylalcohol + O2
acrolein + H2O2
show the reaction diagram
arabitol + O2
?
show the reaction diagram
-
extracellular enzyme, no activity with the intracellular enzyme
-
-
?
benzyl alcohol + O2
benzaldehyde + H2O2
show the reaction diagram
bromoethanol + O2
bromoethanal + H2O2
show the reaction diagram
-
4% of the activity with methanol
-
-
?
butan-1-ol + O2
n-butanal + H2O2
show the reaction diagram
chloroethanol + O2
chloroethanal + H2O2
show the reaction diagram
crotyl alcohol + O2
(2E)-but-2-enal + H2O2
show the reaction diagram
-
-
-
-
?
D-ribose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
decanol + O2
decanal + H2O2
show the reaction diagram
-
-
-
-
?
dodecanol + O2
dodecanal + H2O2
show the reaction diagram
-
-
-
-
?
erythritol + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
ethanol + O2
acetaldehyde + H2O2
show the reaction diagram
ethylene glycol + O2
?
show the reaction diagram
ethylene glycol + O2
? + H2O2
show the reaction diagram
ethylene glycol mono-methylether + O2
methoxyacetaldehyde + H2O2
show the reaction diagram
formaldehyde + methylene blue
formic acid + ?
show the reaction diagram
-
-
-
-
?
formaldehyde + O2
formate + H2O2
show the reaction diagram
formaldehyde + O2
formic acid + H2O2
show the reaction diagram
formaldehyde + p-benzoquinone
formic acid + p-benzoquinol
show the reaction diagram
-
p-benzoquinone, having the highest redox potential, proves to be the most efficient artificial electron acceptor
-
-
?
formaldehyde + toluidine blue
formic acid + ?
show the reaction diagram
-
-
-
-
?
glucose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
glycerol + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
glycerol + O2
glyceraldehyde + H2O2
show the reaction diagram
glycolate + O2
glyoxylate + H2O2
show the reaction diagram
hexadecanol + O2
hexadecanal + H2O2
show the reaction diagram
hexanol + O2
hexanal + H2O2
show the reaction diagram
hydroxyquinone + O2
?
show the reaction diagram
-
-
-
-
?
indole + O2
?
show the reaction diagram
isoamyl alcohol + O2
3-methylbutanal + H2O2
show the reaction diagram
-
-
-
-
?
isoamyl alcohol + O2
? + H2O2
show the reaction diagram
-
2% of the activity with methanol
-
-
?
isobutanol + O2
isobutanal + H2O2
show the reaction diagram
isobutanol + O2
isobutyraldehyde + H2O2
show the reaction diagram
isopropanol + O2
2-propanone + H2O2
show the reaction diagram
L-DOPA + O2
?
show the reaction diagram
L-tryptophan + O2
?
show the reaction diagram
-
-
-
-
?
lactate + O2
decanal + H2O2
show the reaction diagram
-
14% activity compared to glycolate
-
-
?
methanol + O2
acetaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
methanol + O2
formaldehyde + H2O2
show the reaction diagram
n-butanol + O2
butanal + H2O2
show the reaction diagram
n-butanol + O2
butanaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
n-butanol + O2
butyraldehyde + H2O2
show the reaction diagram
-
-
-
-
?
n-butanol + O2
n-butanal + H2O2
show the reaction diagram
n-dodecanol + O2
n-dodecanal + H2O2
show the reaction diagram
n-heptanol + O2
heptanal + H2O2
show the reaction diagram
n-heptanol + O2
heptanaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
n-heptanol + O2
n-heptanal + H2O2
show the reaction diagram
n-hexanol + O2
n-hexanal + H2O2
show the reaction diagram
n-octanol + O2
octanaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
n-pentanol + O2
n-pentanal + H2O2
show the reaction diagram
n-propanol + O2
n-propanal + H2O2
show the reaction diagram
n-propanol + O2
propanal + H2O2
show the reaction diagram
-
-
-
-
?
n-propanol + O2
propanaldehyde + H2O2
show the reaction diagram
n-propanol + O2
propionaldehyde + H2O2
show the reaction diagram
pentan-1-ol + O2
pentanal + H2O2
show the reaction diagram
-
50% of the activity with methanol
-
-
?
pentan-2-ol + O2
pentanone + H2O2
show the reaction diagram
-
56% of the activity with methanol
-
-
?
phenyl-3-propanol + O2
3-phenylpropanal
show the reaction diagram
-
-
-
-
?
prop-2-en-1-ol + O2
acrylaldehyde + H2O2
show the reaction diagram
-
81% of the activity with methanol
-
-
?
propargyl alcohol + O2
propargyl aldehyde + H2O2
show the reaction diagram
propylene glycol + O2
?
show the reaction diagram
-
low activity with intra- and extracellular enzymes
-
-
?
R-(2)-octanol + O2
octan-2-one + H2O2
show the reaction diagram
-
-
-
-
?
ribitol + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
tetradecanol + O2
tetradecanal + H2O2
show the reaction diagram
-
-
-
-
?
veratryl alcohol + O2
?
show the reaction diagram
xylidine + O2
?
show the reaction diagram
-
lowest specific activity with xylidine
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,2-ethanediol + O2
?
show the reaction diagram
-
-
-
-
?
2-methyl-1-butanol
2-methylbutanal + H2O2
show the reaction diagram
-
-
-
-
?
2-methyl-1-hexanol
2-methylhexanal + H2O2
show the reaction diagram
-
-
-
-
?
2-methyl-1-pentanol
2-methylpentanal + H2O2
show the reaction diagram
-
-
-
-
?
3-buten-1-ol + O2
but-3-enal + H2O2
show the reaction diagram
-
-
-
-
?
4-penten-1-ol + O2
pent-4-enal + H2o2
show the reaction diagram
-
-
-
-
?
ethanol + O2
acetaldehyde + H2O2
show the reaction diagram
formaldehyde + O2
formic acid + H2O2
show the reaction diagram
-
-
-
-
?
isopropanol + O2
2-propanone + H2O2
show the reaction diagram
methanol + O2
formaldehyde + H2O2
show the reaction diagram
n-butanol + O2
butanaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
n-butanol + O2
n-butanal + H2O2
show the reaction diagram
-
-
-
-
?
n-heptanol + O2
heptanaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
n-octanol + O2
octanaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
n-pentanol + O2
n-pentanal + H2O2
show the reaction diagram
-
-
-
-
?
n-propanol + O2
n-propanal + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe3+
-
the enzyme contains 2 iron atoms and shows 112% activity at 1 mM Fe3+
K+
-
slight activation of intracellular enzyme
MgCl2
-
1 mM, increases the enzyme activity to nearly 50% for both short chain-, and long chain alcohol substrates
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10 phenanthroline
-
-
1,10-phenanthroline
1,12-dichlorododecane
-
-
1,4-butynediol
2,2'-dipyridyl
-
1 mM, 13% inhibition
2-Aminoethanol
-
1 mM, 70% inhibition
2-mercaptoethanol
-
-
3-Pentanol
-
1 mM, 16% inhibition
4-chloromercuribenzoate
-
0.1 mM, 31% inhibition
4-Hydroxy-2-butynal
5,5'-dithiobis(2-nitrobenzoate)
5,5'-dithiobis-(2-nitrobenzoic acid)
-
-
8-hydroxyquinoline
-
1 mM, 6% inhibition
acetamide
AgNO3
-
1 mM, complete loss of activity
Cyclopropanol
-
-
Cyclopropanone
diethyldicarbonate
dithiothreitol
-
85% residual activity at 0.5 mM
DMSO
-
complete inhibition by 50% DMSO
Fe2+
-
slight inhibition of extracellular enzyme
FeSO4
formaldehyde
HgCl2
-
1 mM, 44% inhibition
hydrazine
hydroquinone
hydroxylamine
imidazole
-
1 mM, 20% inhibition
iodoacetate
iodoacetic acid
-
59% residual activity at 1 mM
KBr
-
3.5 M at 30C for 1-2 h: partial inactivation, KBr + urea: complete inactivation
L-cysteine
-
70% residual activity at 0.5 mM
Mercuric acetate
-
0.1 mM, complete inhibition
Mercuric chloride
-
1 mM, complete inhibition
Metal chelators
-
slight
-
methanol
-
substrate inhibition
Mo6+
-
1 mM, 94% inhibition
Monoiodoacetic acid
-
1 mM, 31% inhibition
N-ethylmaleimide
-
84% residual activity at 1 mM
Na+
-
slight inhibition of extracellular enzyme
NiCl2
o-phenanthroline
-
93% residual activity at 1 mM
p-chloromercuribenzoate
Pichia putida
-
complete inhibition at molar excess of reagents in relation to alcohol oxidase 20:1
p-chloromercuribenzoic acid
-
-
p-hydroxymercuribenzoate
-
1 mM, complete inhibition
phenylhydrazine
propargyl alcohol
-
irreversible
Propynal
Semicarbazide
-
91% residual activity at 1 mM
Sodium acetate
Sodium azide
Tetraethylthiuram disulfide
-
5 mM, more than 70% loss of activity
Thiosemicarbazide
-
1 mM, 31% loss of activity
Urea
-
6 M, at 30C for 1-2 h: partial inactivation, urea + KBr: complete inactivation
Zn2+
-
35% residual activity at 0.5 mM
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
1 mM, 1.25fold activation
Ferrocene
-
1 mM, increases the alcohol oxidase activity only for the short chain alcohol substrate to 47% of the original activity
gold nanoparticle
-
the catalytic efficiency of gold nanoparticle-conjugated enzyme is increased by 18% from the free enzyme
-
iodoacetamide
-
1 mM, 1.3fold activation
methanol
-
induces enzyme expression at 3%
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
18.3
(R)-2-octanol
-
-
36
1,4-butynediol
-
-
0.0109
1-Decanol
-
at pH 5.5 and 30C
0.41
1-Hexanol
-
at pH 5.5 and 30C
0.0365
1-Octanol
-
at pH 5.5 and 30C
5.54
1-Pentanol
-
at pH 5.5 and 30C
42.7
1-propanol
at pH 9.0 and 50C; at pH 9.0 and 50C
27.2
2-mercaptoethanol
Poria contigua
-
-
261
2-propanol
-
pH 6.5, 22C, immobilized enzyme
3.2 - 10
2-propin-1-ol
0.18
4-hepten-1-ol
-
-
0.44
4-Hydroxy-2-butynal
-
-
0.42
4-nonen-1-ol
-
-
33.3
allyl alcohol
-
-
26.6
benzyl alcohol
-
-
0.075
butanol
-
-
71.5
D-ribose
-
-
0.018
Decanol
-
-
0.038
Dodecanol
-
-
670
erythritol
-
-
0.13 - 22.5
ethanol
0.95 - 9
ethylene glycol
1.59 - 10.5
formaldehyde
1.9
glycerol
at pH 9.0 and 50C
167
glycolate
-
at pH 5.5 and 30C
0.0005 - 0.023
hexadecanol
0.029
Hexanol
-
-
25
Isopropanol
Poria contigua
-
-
15.8
Lactate
-
at pH 5.5 and 30C
0.019 - 89.58
methanol
0.028 - 166
n-butanol
1.86
n-dodecanol
-
-
0.005 - 0.498
n-Heptanol
0.05
n-Octanol
-
pH 8.5, 30C, SCAO
10.9
n-Pentanol
at pH 9.0 and 50C; at pH 9.0 and 50C
1.23 - 66
n-Propanol
0.34 - 1
O2
0.0032
Octanol
-
-
0.05
p-benzoquinone
-
-
2.3
phenyl-3-propanol
-
-
0.067
Propanol
-
-
250
ribitol
-
-
0.0056
Tetradecanol
-
-
additional information
additional information
-