Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | 6-phosphogluconate modulates 6PGDH activity. In the forward reaction, it suppresses inhibition by NADP and decreases inhibition by NADPH by increasing the Kd of the reduced coenzyme 6fold it increases the rate of decarboxylation of the 3-keto intermediate , whereas in the reverse reaction, it decreases the Km and removes the slow step | Cyberlindnera jadinii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
6-phospho-D-gluconate | dimeric enzyme with two 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication | Cyberlindnera jadinii | |
0.07 | - |
6-phospho-D-gluconate | dimeric enzyme with one 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication | Cyberlindnera jadinii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera jadinii | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH + H+ | reductive carboxylation of ribulose-5-phosphate by 6PGDH is investigated using kinetic isotope effects. In the absence of 6-phosphogluconate, a slow step, which precedes the chemical process, is rate-limiting for the reaction, whereas in the presence of 6-phosphogluconate, the rate-limiting step follows the isotope-sensitive step. Full activity of 6PGDH is achieved when one subunit carries out the catalysis and the other subunit carries an unreacted 6-phosphogluconate (allosteric activator) | Cyberlindnera jadinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NADP+ | 6-phosphogluconate modulates 6PGDH activity. In the forward reaction, it suppresses inhibition by NADP and decreases inhibition by NADPH by increasing the Kd of the reduced coenzyme 6fold it increases the rate of decarboxylation of the 3-keto intermediate , whereas in the reverse reaction, it decreases the Km and removes the slow step | Cyberlindnera jadinii | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
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Synonyms | Comment | Organism |
---|---|---|
6PGDH | - |
Cyberlindnera jadinii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Cyberlindnera jadinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
assay at | Cyberlindnera jadinii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Cyberlindnera jadinii |