Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | [Ruminococcus] torques |
Protein Variants | Comment | Organism |
---|---|---|
G57S/I163V/V207M | the mutant shows increased catalytic efficiency compared to the wild type enzyme | [Ruminococcus] torques |
I112V/N240D | the mutant shows increased catalytic efficiency compared to the wild type enzyme | [Ruminococcus] torques |
I112V/T189I/N240D | the mutant shows strongly decreased catalytic efficiency compared to the wild type enzyme | [Ruminococcus] torques |
I28V/V38A/I76V/V207M | the mutant shows increased catalytic efficiency compared to the wild type enzyme | [Ruminococcus] torques |
K44E/T189I | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | [Ruminococcus] torques |
N240I | the mutant shows increased catalytic efficiency compared to the wild type enzyme | [Ruminococcus] torques |
T189V | the mutant shows increased catalytic efficiency compared to the wild type enzyme | [Ruminococcus] torques |
T189V/V207M | the mutant shows increased catalytic efficiency compared to the wild type enzyme | [Ruminococcus] torques |
V207M | the mutant shows increased catalytic efficiency compared to the wild type enzyme | [Ruminococcus] torques |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0078 | - |
NADPH | wild type enzyme, at pH 8.0 and 30°C | [Ruminococcus] torques | |
0.021 | - |
7-oxo-lithocholic acid | mutant enzyme I28V/V38A/I76V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
0.023 | - |
7-oxo-lithocholic acid | wild type enzyme, at pH 8.0 and 30°C | [Ruminococcus] torques | |
0.023 | - |
7-oxo-lithocholic acid | mutant enzyme N240I, at pH 8.0 and 30°C | [Ruminococcus] torques | |
0.028 | - |
7-oxo-lithocholic acid | mutant enzyme I112V/N240D, at pH 8.0 and 30°C | [Ruminococcus] torques | |
0.031 | - |
7-oxo-lithocholic acid | mutant enzyme T189V, at pH 8.0 and 30°C | [Ruminococcus] torques | |
0.034 | - |
7-oxo-lithocholic acid | mutant enzyme G57S/I163V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
0.035 | - |
7-oxo-lithocholic acid | mutant enzyme V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
0.04 | - |
7-oxo-lithocholic acid | mutant enzyme T189V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
0.047 | - |
NADPH | mutant enzyme T189V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
0.057 | - |
7-oxo-lithocholic acid | mutant enzyme K44E/T189I, at pH 8.0 and 30°C | [Ruminococcus] torques | |
0.19 | - |
7-oxo-lithocholic acid | mutant enzyme I112V/T189I/N240D, at pH 8.0 and 30°C | [Ruminococcus] torques |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Ruminococcus] torques | - |
- |
- |
[Ruminococcus] torques ATCC 35915 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-oxo-lithocholic acid + NADPH + H+ | - |
[Ruminococcus] torques | ursodeoxycholic acid + NADP+ | - |
? | |
7-oxo-lithocholic acid + NADPH + H+ | - |
[Ruminococcus] torques ATCC 35915 | ursodeoxycholic acid + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
7beta-HSDH | - |
[Ruminococcus] torques |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
melting temperature | [Ruminococcus] torques |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
7-oxo-lithocholic acid | mutant enzyme I112V/T189I/N240D, at pH 8.0 and 30°C | [Ruminococcus] torques | |
4.2 | - |
7-oxo-lithocholic acid | wild type enzyme, at pH 8.0 and 30°C | [Ruminococcus] torques | |
4.3 | - |
NADPH | wild type enzyme, at pH 8.0 and 30°C | [Ruminococcus] torques | |
7.8 | - |
7-oxo-lithocholic acid | mutant enzyme K44E/T189I, at pH 8.0 and 30°C | [Ruminococcus] torques | |
13.4 | - |
7-oxo-lithocholic acid | mutant enzyme N240I, at pH 8.0 and 30°C | [Ruminococcus] torques | |
17.3 | - |
7-oxo-lithocholic acid | mutant enzyme V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
17.5 | - |
7-oxo-lithocholic acid | mutant enzyme I28V/V38A/I76V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
20.1 | - |
7-oxo-lithocholic acid | mutant enzyme T189V, at pH 8.0 and 30°C | [Ruminococcus] torques | |
21.4 | - |
7-oxo-lithocholic acid | mutant enzyme I112V/N240D, at pH 8.0 and 30°C | [Ruminococcus] torques | |
23 | - |
7-oxo-lithocholic acid | mutant enzyme T189V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
25.1 | - |
NADPH | mutant enzyme T189V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
31 | - |
7-oxo-lithocholic acid | mutant enzyme G57S/I163V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
[Ruminococcus] torques |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
[Ruminococcus] torques |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
7-oxo-lithocholic acid | mutant enzyme I112V/T189I/N240D, at pH 8.0 and 30°C | [Ruminococcus] torques | |
140 | - |
7-oxo-lithocholic acid | mutant enzyme K44E/T189I, at pH 8.0 and 30°C | [Ruminococcus] torques | |
180 | - |
7-oxo-lithocholic acid | wild type enzyme, at pH 8.0 and 30°C | [Ruminococcus] torques | |
480 | - |
7-oxo-lithocholic acid | mutant enzyme V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
530 | - |
NADPH | mutant enzyme T189V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
550 | - |
NADPH | wild type enzyme, at pH 8.0 and 30°C | [Ruminococcus] torques | |
580 | - |
7-oxo-lithocholic acid | mutant enzyme N240I, at pH 8.0 and 30°C | [Ruminococcus] torques | |
580 | - |
7-oxo-lithocholic acid | mutant enzyme T189V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
640 | - |
7-oxo-lithocholic acid | mutant enzyme T189V, at pH 8.0 and 30°C | [Ruminococcus] torques | |
760 | - |
7-oxo-lithocholic acid | mutant enzyme I112V/N240D, at pH 8.0 and 30°C | [Ruminococcus] torques | |
830 | - |
7-oxo-lithocholic acid | mutant enzyme I28V/V38A/I76V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques | |
910 | - |
7-oxo-lithocholic acid | mutant enzyme G57S/I163V/V207M, at pH 8.0 and 30°C | [Ruminococcus] torques |