Information on EC 1.1.1.201 - 7beta-hydroxysteroid dehydrogenase (NADP+)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.201
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RECOMMENDED NAME
GeneOntology No.
7beta-hydroxysteroid dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 7beta-hydroxysteroid + NADP+ = a 7-oxosteroid + NADPH + H+
show the reaction diagram
; Catalyses the oxidation of the 7beta-hydroxyl group of bile salts such as ursodeoxycholate
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycocholate metabolism (bacteria)
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Secondary bile acid biosynthesis
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ursodeoxycholate biosynthesis (bacteria)
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SYSTEMATIC NAME
IUBMB Comments
7beta-hydroxysteroid:NADP+ 7-oxidoreductase
Catalyses the oxidation of the 7beta-hydroxy group of bile acids such as ursodeoxycholate.
CAS REGISTRY NUMBER
COMMENTARY hide
79393-83-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 25.11.c
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Manually annotated by BRENDA team
strain 25.11.c
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,7,12-triketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-3,12-diketo-5beta-cholanoic acid + NADP+
show the reaction diagram
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-
-
?
3,7,12-triketo-5beta-cholanoyl taurine + NADPH
7beta-hydroxy-3,12-diketo-5beta-cholanoyl taurine + NADP+
show the reaction diagram
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-
-
?
3,7-diketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+
show the reaction diagram
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
show the reaction diagram
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
show the reaction diagram
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
show the reaction diagram
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha,7keto-dihydroxy-5beta-cholanoic acid + NADPH
show the reaction diagram
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
show the reaction diagram
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
show the reaction diagram
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH
3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+
show the reaction diagram
5-cholesten-3beta,7beta-diol + NADP+
5-cholesten-3beta-ol-7-one + NADPH
show the reaction diagram
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i.e. 7beta-hydroxycholesterol
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?
7,12-diketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+
show the reaction diagram
7-ketolithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
show the reaction diagram
7beta-hydroxycholesterol + NADP+
7-oxocholesterol + NADPH
show the reaction diagram
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?
corticosterone + NADP+
21-hydroxy-pregn-4-ene-3,11,20-trione + NADPH
show the reaction diagram
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oxidation at C-11
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?
cortisone + NADP+
? + NADPH
show the reaction diagram
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oxidation at C-11
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?
dehydrocholic acid + NADPH + H+
? + NADP+ + H+
show the reaction diagram
ursodeoxycholic acid + NADP+
7-ketolithocholic acid + NADPH + H+
show the reaction diagram
additional information
?
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enzyme might also show 11beta-hydroxysteroid dehydrogenase activity, no activity with cholesterol, 7alpha-hydroxycholesterol, 25-hydroxycholesterol, pregnenolone, dehydroepiandrosterone, chenodoxycolic acid, and ursodeoxycholic acid
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3,7,12-triketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-3,12-diketo-5beta-cholanoic acid + NADP+
show the reaction diagram
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-
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?
3,7,12-triketo-5beta-cholanoyl taurine + NADPH
7beta-hydroxy-3,12-diketo-5beta-cholanoyl taurine + NADP+
show the reaction diagram
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-
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?
3,7-diketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+
show the reaction diagram
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
show the reaction diagram
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
show the reaction diagram
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha,7keto-dihydroxy--5beta-cholanoic acid + NADPH
show the reaction diagram
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
show the reaction diagram
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
show the reaction diagram
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH
3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+
show the reaction diagram
7,12-diketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+
show the reaction diagram
7beta-hydroxycholesterol + NADP+
7-oxocholesterol + NADPH
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetate
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complete inhibition at 0.05 mM
p-chloromercuribenzoic acid
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complete inhibition at 0.001 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07
3,12-diketocholanoic acid
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0.123
3,7,12-Triketo-5beta-cholanoic acid
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0.145
3,7,12-triketo-5beta-cholanoyl taurine
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0.087
3,7-Diketo-5beta-cholanoic acid
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0.142
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid
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0.076
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid
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0.009 - 0.108
3alpha,7beta-dihydroxy-5beta-cholanoic acid
0.238 - 0.909
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine
0.286 - 1.639
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine
0.124
3alpha-Hydroxy-7,12-diketo-5beta-cholanoic acid
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0.055
3alpha-hydroxy-7-keto-5beta-cholanoic acid
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0.0052
7-ketolithocholic acid
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pH 8.0, 25°C
0.0092
Dehydrocholic acid
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pH 8.0, 25°C
0.0053 - 0.4
NADP+
0.0045
NADPH
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pH 8.0, 25°C
0.0062
Ursodeoxycholic acid
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pH 8.0, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15.8
7-ketolithocholic acid
Collinsella aerofaciens
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pH 8.0, 25°C
14.6
Dehydrocholic acid
Collinsella aerofaciens
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pH 8.0, 25°C
15.7
NADP+
Collinsella aerofaciens
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pH 8.0, 25°C
17.2
NADPH
Collinsella aerofaciens
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pH 8.0, 25°C
19.6
Ursodeoxycholic acid
Collinsella aerofaciens
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pH 8.0, 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.1
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crude cell extract
0.276
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purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.7
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potassium buffer
9.5 - 10.5
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9.8 - 10.5
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11
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glycine-NaOH buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
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2 * 30000, SDS-PAGE
34000
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x * 34000, SDS-PAGE
45000
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gel filtration
53000
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gel filtration
56100
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gel filtration
82000
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gel filtration
115000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 34000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
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50% loss of activity within 60 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
heat-sensitive
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presence of reducing agents protects against thermal denaturation
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50 mM potassium phosphate, pH 8.0, stable for at least 3 months with several times of freezing and thawing
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme from liver microsomes 300fold by ion exchange, hydroxylapatite, 2',5'-ADP affinity
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to homogeneity, chromatography techniques
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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