Information on EC 2.7.7.39 - glycerol-3-phosphate cytidylyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.7.39
-
RECOMMENDED NAME
GeneOntology No.
glycerol-3-phosphate cytidylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
poly(3-O-beta-D-glucopyranosyl-N-acetylgalactosamine 1-phosphate) wall teichoic acid biosynthesis
-
-
poly(glycerol phosphate) wall teichoic acid biosynthesis
-
-
poly(ribitol phosphate) wall teichoic acid biosynthesis I (B. subtilis)
-
-
poly(ribitol phosphate) wall teichoic acid biosynthesis II (S. aureus)
-
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lipid metabolism
-
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teichoic acid biosynthesis
-
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Glycerophospholipid metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
CTP:sn-glycerol-3-phosphate cytidylyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9027-11-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ATCC 9945
-
-
Manually annotated by BRENDA team
W23
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
show the reaction diagram
dCTP + glycerol 3-phosphate
diphosphate + dCDPglycerol
show the reaction diagram
-
about 95% of the activity with CTP
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CDPglycerol
diphosphate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
UDP-N-acetylglucosamine
-
stimulates, up to 5 mM
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine
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slight stimulation below 2.5 mM, inhibition above
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
60
CDPglycerol
-
pH 8, reverse reaction
0.036 - 93.5
CTP
9.7
diphosphate
-
pH 8, reverse reaction
0.0014 - 141
glycerol 3-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
CDPglycerol
Staphylococcus aureus
-
pH 8, reverse reaction
0.001 - 26.2
CTP
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9 - 9.5
-
-
7
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Tis-maleate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30500
-
conditional lethal mutant 168, gel filtration
30900
-
gene expression in E. coli, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
complexed with CDPglycerol
determination of the crystal structure of TarDSa in the apo state to 3 A resolution
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hanging drop vapor diffusion method
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
37C, 4 h, about 30% loss of activity
645242
7.5 - 9.5
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37C, 4 h, stable
645242
10
-
37C, 4 h, about 10% loss of activity
645242
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
not stable for longer than 30 min
additional information
-
thermostability of enzyme gro-PCT in extract of strains bearing mutations in tagA, tagB and tagF genes
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, about 20% loss of activity after 1 month
-
-80C, pure enzyme stable for at least 7 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene expression in E. coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene expressed in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C106A
-
mutant with kinetic constants similar to wild-type enzyme
D11A
-
mutant with low kcat value
D11E
-
mutant with low kcat value
D38A
-
mutant with high Km value
D38E
-
kcat similar to wild-type enzyme
D66A
-
mutant with low kcat value, defective in overall structure
D66E
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mutant with low kcat value
D87A
-
mutant with kinetic constants similar to wild-type enzyme
D92A
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mutant with kinetic constants similar to wild-type enzyme
D94A/H14A
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binding constants for CTP and glycerol 3-phosphate are similar to wild-type enzyme
D94E mutant
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mutant with low kcat value
E115A
-
mutant with kinetic constants similar to wild-type enzyme
E39A
-
mutant with kinetic constants similar to wild-type enzyme
E67A
-
mutant with kinetic constants similar to wild-type enzyme
H84A
-
mutant with low kcat value
K103A
-
mutant with kinetic constants similar to wild-type enzyme
K19A
-
mutant with kinetic constants similar to wild-type enzyme
K22A
-
mutant with kinetic constants similar to wild-type enzyme
K25A
-
mutant with kinetic constants similar to wild-type enzyme
K44A
less active than wild-type enzyme
K46A
less active than wild-type enzyme
R113A
-
mutant with low kcat value
R113K
-
mutant with low kcat value
R55A
-
mutant with low kcat value
R55K
-
mutant with low kcat value
R63A
-
mutant with low kcat value
S118A
-
mutant with low kcat value t
T114A
-
mutant with low kcat value
T119A
-
mutant with low kcat value
W74A
-
mutant with high Km value
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