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Literature summary for 2.7.7.39 extracted from
- Expression, purification, and characterization of CTP:glycerol-3-phosphate cytidylyltransferase from Bacillus subtilis (1993), J. Biol. Chem., 268, 16648-16654.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene expressed in Escherichia coli | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Bacillus subtilis |  |
| CDPglycerol | - |
Bacillus subtilis | |
| Cu2+ | - |
Bacillus subtilis | |
| diphosphate | - |
Bacillus subtilis | |
| Hg2+ | - |
Bacillus subtilis | |
| Sn2+ | - |
Bacillus subtilis | |
| Zn2+ | - |
Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.23 | - |
glycerol 3-phosphate | pH 8, 37°C | Bacillus subtilis | |
| 3.85 | - |
CTP | pH 8, 37°C | Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required | Bacillus subtilis | |
| Fe2+ | divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required | Bacillus subtilis | |
| Mg2+ | divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required | Bacillus subtilis | |
| Mn2+ | divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14800 | - |
2 * 14800, gene expression in Escherichia coli, SDS-PAGE | Bacillus subtilis |
| 15271 | - |
2 * 15271, calculated from the nucleotide sequence | Bacillus subtilis |
| 30900 | - |
gene expression in E. coli, gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + sn-glycerol 3-phosphate | Bacillus subtilis | enzyme of teichoic acid synthesis | diphosphate + CDPglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
BR151, gene expressed in Escherichia coli | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gene expression in E. coli | Bacillus subtilis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol | rapid equilibrium random order mechanism | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 85.7 | - |
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, about 20% loss of activity after 1 month | Bacillus subtilis |
| -80°C, pure enzyme stable for at least 7 months | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + sn-glycerol 3-phosphate | - |
Bacillus subtilis | diphosphate + CDPglycerol | - |
? | |
| CTP + sn-glycerol 3-phosphate | enzyme of teichoic acid synthesis | Bacillus subtilis | diphosphate + CDPglycerol | - |
? | |
| dCTP + glycerol 3-phosphate | about 95% of the activity with CTP | Bacillus subtilis | diphosphate + dCDPglycerol | - |
? | |
| additional information | no substrate: GTP | Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 14800, gene expression in Escherichia coli, SDS-PAGE | Bacillus subtilis |
| dimer | 2 * 15271, calculated from the nucleotide sequence | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus subtilis |
| 50 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
not stable for longer than 30 min | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 9.5 | - |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
37°C, 4 h, about 30% loss of activity | Bacillus subtilis |
| 7.5 | 9.5 | 37°C, 4 h, stable | Bacillus subtilis |
| 10 | - |
37°C, 4 h, about 10% loss of activity | Bacillus subtilis |
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