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2'-dATP + D-galactose
2'-dADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
2-amino-2-deoxy-D-galactose + ATP
2-amino-2-deoxy-D-galactose 1-phosphate + ADP
-
-
-
-
?
2-deoxy-D-galactose + ATP
2-deoxy-D-galactose 1-phosphate + ADP
-
-
-
-
?
3'-dATP + D-galactose
3'-dADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
3-deoxy-D-galactose + ATP
?
-
-
-
-
?
ADP + alpha-D-galactose 1-phosphate
ATP + alpha-D-galactose
-
-
-
-
r
alpha-D-galactose + ATP
alpha-D-galactose 1-phosphate + ADP
-
-
-
-
?
ATP + 2-amino-2-deoxy-D-galactose
ADP + 2-amino-2-deoxy-D-galactose 1-phosphate
-
-
-
-
?
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-alpha-D-galactose 1-phosphate
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-D-galactose 1-phosphate
ATP + 3-deoxy-3-fluorogalactose
ADP + 3-deoxy-3-fluorogalactose 1-phosphate
ATP + 3-deoxy-3-methyl-D-galactose
ADP + 3-deoxy-3-methyl-D-galactose 1-phosphate
-
-
-
-
?
ATP + 3-deoxy-D-galactose
ADP + 3-deoxy-D-galactose 1-phosphate
ATP + 4-deoxy-4-fluorogalactose
ADP + 4-deoxy-4-fluorogalactose 1-phosphate
21% conversion
-
-
?
ATP + 4-deoxy-D-galactose
ADP + 4-deoxy-alpha-D-galactose 1-phosphate
-
substrate only for mutants Y223F, Y223W
-
-
?
ATP + 6-deoxy-6-fluorogalactose
ADP + 6-deoxy-6-fluorogalactose 1-phosphate
28% conversion
-
-
?
ATP + 6-deoxy-D-galactose
ADP + 6-deoxy-alpha-D-galactose 1-phosphate
ATP + 6-deoxy-D-galactose
ADP + 6-deoxy-D-galactose 1-phosphate
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
ATP + D-galactosamine
ADP + alpha-D-galactosamine 1-phosphate
ATP + D-galactosamine
ADP + D-galactosamine 1-phosphate
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
ATP + D-galactose
ADP + D-galactose 1-phosphate
-
-
-
?
ATP + D-galacturonic acid
ADP + alpha-D-galacturonic acid 1-phosphate
-
substrate: galacturonic acid, specific activity: 0.09 micromol/min/mg
-
-
?
ATP + D-galacturonic acid
ADP + D-galacturonic acid 1-phosphate
ATP + D-glucose
ADP + alpha-D-glucose 1-phosphate
ATP + D-talose
ADP + alpha-D-talose 1-phosphate
ATP + galactose
ADP + alpha-D-galactose 1-phosphate
ATP + L-altrose
ADP + L-altrose 1-phosphate
-
-
-
-
?
ATP + L-arabinose
ADP + L-arabinose 1-phosphate
10% conversion after 2 h under optimised conditions
-
-
?
ATP + L-glucose
ADP + alpha-L-glucose 1-phosphate
-
substrate only for mutants Y223F, Y223W
-
-
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
CTP + D-galactose
CDP + alpha-D-galactose 1-phosphate
-
substrate: CTP, specific activity: 0.551 micromol/min/mg
-
-
?
D-fucose + ATP
?
-
-
-
-
?
D-galactosamine + ATP
?
-
-
-
-
?
D-talose + ATP
alpha-D-talose 1-phosphate + ADP
-
-
-
-
?
dATP + D-galactose
dADP + alpha-D-galactose 1-phosphate
-
substrate: dATP, specific activity: 138 micromol/min/mg
-
-
?
dCTP + D-galactose
dCDP + alpha-D-galactose 1-phosphate
-
substrate: dCTP, specific activity: 0.931 micromol/min/mg
-
-
?
deoxy-ATP + D-galactose
deoxyADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
dGTP + D-galactose
dGDP + alpha-D-galactose 1-phosphate
-
substrate: dGTP, specific activity: 31.9 micromol/min/mg
-
-
?
dTTP + D-galactose
?
-
substrate: dTTP, specific activity: 2.5 micromol/min/mg
-
-
?
dUTP + D-galactose
dUDP + alpha-D-galactose 1-phosphate
-
substrate: dUTP, specific activity: 1.5 micromol/min/mg
-
-
?
GTP + D-galactose
GDP + alpha-D-galactose 1-phosphate
-
substrate: GTP, specific activity: 28.5 micromol/min/mg
-
-
?
ITP + D-galactose
IDP + alpha-D-galactose 1-phosphate
-
substrate: ITP, specific activity: 15.7 micromol/min/mg
-
-
?
UTP + D-galactose
UDP + alpha-D-galactose 1-phosphate
-
substrate: UTP, specific activity: 0.52 micromol/min/mg
-
-
?
additional information
?
-
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
substrate: 2-deoxygalactose, specific activity: 99.7 micromol/min/mg
-
-
?
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
-
-
?
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-D-galactose 1-phosphate
-
-
-
-
?
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-D-galactose 1-phosphate
-
-
-
?
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-D-galactose 1-phosphate
-
-
-
-
?
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-D-galactose 1-phosphate
-
-
-
-
?
ATP + 2-deoxy-D-galactose
ADP + 2-deoxy-D-galactose 1-phosphate
-
-
-
-
?
ATP + 3-deoxy-3-fluorogalactose
ADP + 3-deoxy-3-fluorogalactose 1-phosphate
27% conversion
-
-
?
ATP + 3-deoxy-3-fluorogalactose
ADP + 3-deoxy-3-fluorogalactose 1-phosphate
27% conversion
-
-
?
ATP + 3-deoxy-D-galactose
ADP + 3-deoxy-D-galactose 1-phosphate
19% conversion
-
-
?
ATP + 3-deoxy-D-galactose
ADP + 3-deoxy-D-galactose 1-phosphate
85% conversion after 2 h under optimised conditions
-
-
?
ATP + 3-deoxy-D-galactose
ADP + 3-deoxy-D-galactose 1-phosphate
85% conversion after 2 h under optimised conditions
-
-
?
ATP + 6-deoxy-D-galactose
ADP + 6-deoxy-alpha-D-galactose 1-phosphate
-
substrate: 6-deoxygalactose, specific activity: 0.0781 micromol/min/mg
-
-
?
ATP + 6-deoxy-D-galactose
ADP + 6-deoxy-alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + 6-deoxy-D-galactose
ADP + 6-deoxy-D-galactose 1-phosphate
-
-
-
-
?
ATP + 6-deoxy-D-galactose
ADP + 6-deoxy-D-galactose 1-phosphate
57% conversion
-
-
?
ATP + 6-deoxy-D-galactose
ADP + 6-deoxy-D-galactose 1-phosphate
57% conversion
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
the enzyme catalyses the first committed step in the Leloir pathway
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
negative charge at residue 174 is essential for catalysis
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
r
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
72% conversion
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
72% conversion
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
involved in Leloir pathway
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
91% conversion after 2 h under optimised conditions
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
involved in Leloir pathway
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
91% conversion after 2 h under optimised conditions
-
-
?
ATP + alpha-D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactosamine
ADP + alpha-D-galactosamine 1-phosphate
-
substrate: D-galactosamine, specific activity: 12.5 micromol/min/mg
-
-
?
ATP + D-galactosamine
ADP + alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + D-galactosamine
ADP + alpha-D-galactosamine 1-phosphate
68% conversion
-
-
?
ATP + D-galactosamine
ADP + alpha-D-galactosamine 1-phosphate
68% conversion
-
-
?
ATP + D-galactosamine
ADP + D-galactosamine 1-phosphate
100% of the activity with alpha-D-galactose
-
-
?
ATP + D-galactosamine
ADP + D-galactosamine 1-phosphate
100% of the activity with alpha-D-galactose
-
-
?
ATP + D-galactosamine
ADP + D-galactosamine 1-phosphate
-
-
-
-
?
ATP + D-galactosamine
ADP + D-galactosamine 1-phosphate
-
-
-
?
ATP + D-galactosamine
ADP + D-galactosamine 1-phosphate
87% conversion after 2 h under optimised conditions
-
-
?
ATP + D-galactosamine
ADP + D-galactosamine 1-phosphate
87% conversion after 2 h under optimised conditions
-
-
?
ATP + D-galactosamine
ADP + D-galactosamine 1-phosphate
-
-
-
-
?
ATP + D-galactosamine
ADP + D-galactosamine 1-phosphate
-
-
-
-
?
ATP + D-galactosamine
ADP + D-galactosamine 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
very strict substrate specificity for galactose and ATP
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
substrate: ATP, specific activity: 137 micromol/min/mg
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
substrate: D-galactose, specific activity: 158 micromol/min/mg
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
unidirectional towards galactose phosphorylation
-
-
ir
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
ir
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
ir
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + D-galacturonic acid
ADP + D-galacturonic acid 1-phosphate
20.2% of the activity with alpha-D-galactose
-
-
?
ATP + D-galacturonic acid
ADP + D-galacturonic acid 1-phosphate
20.2% of the activity with alpha-D-galactose
-
-
?
ATP + D-glucose
ADP + alpha-D-glucose 1-phosphate
52.5% of the activity with alpha-D-galactose
-
-
?
ATP + D-glucose
ADP + alpha-D-glucose 1-phosphate
52.5% of the activity with alpha-D-galactose
-
-
?
ATP + D-glucose
ADP + alpha-D-glucose 1-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + alpha-D-glucose 1-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + alpha-D-glucose 1-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + alpha-D-glucose 1-phosphate
-
-
-
-
?
ATP + D-talose
ADP + alpha-D-talose 1-phosphate
-
substrate only for mutants Y223F, Y223W
-
-
?
ATP + D-talose
ADP + alpha-D-talose 1-phosphate
-
-
-
?
ATP + D-talose
ADP + alpha-D-talose 1-phosphate
-
-
-
-
?
ATP + D-talose
ADP + alpha-D-talose 1-phosphate
41% conversion
-
-
?
ATP + D-talose
ADP + alpha-D-talose 1-phosphate
41% conversion
-
-
?
ATP + galactose
ADP + alpha-D-galactose 1-phosphate
-
the enzyme catalyzes the first step in the conversion of galactose to glucose. Galactokinase deficiency is characterized by galactosemia, galactosuria and cataract formation in early childhood
-
-
?
ATP + galactose
ADP + alpha-D-galactose 1-phosphate
-
galactokinase deficiency is an autosomal recessive disorder, which causes cataract formation in children not maintained on a lactose-free diet
-
-
?
ATP + galactose
ADP + alpha-D-galactose 1-phosphate
-
galaktokinase deficiency is an autosomal recessive disorder characterized by hypergalactosemia and cataract formation
-
-
?
ATP + galactose
ADP + alpha-D-galactose 1-phosphate
-
lack of fully functional galactokinase is one cause of the inherited disease galactosemia, the main clinical manifestation of which is the early onset of cataracts
-
-
?
ATP + galactose
ADP + alpha-D-galactose 1-phosphate
-
-
-
-
?
ATP + galactose
ADP + alpha-D-galactose 1-phosphate
-
first step in the Leloir pathway for galactose metabolism
-
-
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
15.5% of the activity with alpha-D-galactose
-
-
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
15.5% of the activity with alpha-D-galactose
-
-
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
-
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
40% conversion after 2 h under optimised conditions
-
-
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
40% conversion after 2 h under optimised conditions
-
-
?
additional information
?
-
-
no tolerance for C-4 changes in the substrate
-
-
?
additional information
?
-
-
no substrate: N-acetyl-D-galactosamine, L-arabinose, D-fucose, D-glucose
-
-
?
additional information
?
-
-
amino acid side chains responsible for anchoring the sugar ligand to the protein include Arg36, Glu42, Asp45, ASp183, and Tyr233
-
-
?
additional information
?
-
-
mechanism of the reaction is sequential in both directions. Residue Asp183 acts as a catalytic base that deprotonates the C1 hydroxyl group of galactose. The pH-kcat profile of the synthesis of galactose 1-phosphate has a pKa of 6.9 likely due to Asp183. The pH-kcat/KGal profile of the reverse reaction lacks a key pKa required for a direct proton transfer mechanism. Arg36 may lower the pKa of the C1 hydroxyl to facilitate deprotonation
-
-
?
additional information
?
-
-
galactokinase Gal1p is a regulator of transcription. Gal1p activates Gal4p, the activator of GAL genes. Gal1p activates Gal4p by direct interaction with the Gal4p inhibitor Gal80p. Interaction requires galactose, adenosine triphosphate and the regulatory function of Gal1p. The gal1p-gal80p complex formation results in the inactivation of Gal80p, thereby transmitting the galactose signal to Gal4p
-
-
?
additional information
?
-
-
L-arabinose, D-fucose, L-glucose, and D-talose are no substrates
-
-
?
additional information
?
-
-
L-arabinose, D-fucose, L-glucose, and D-talose are no substrates
-
-
?
additional information
?
-
-
key enzyme for cellulase induction during growth on lactose
-
-
?
additional information
?
-
-
induction of the gal pathway and cellulase genes involves no transcriptional inducer function of the galactokinase in Hypocrea jecorina
-
-
?
additional information
?
-
-
induction of the gal pathway and cellulase genes involves no transcriptional inducer function of the galactokinase in Hypocrea jecorina
-
-
?
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2.9
2-amino-deoxy-D-galactose
-
-
0.3 - 29.21
2-deoxy-D-galactose
6.4
3-deoxy-3--methyl-D-galactose
-
-
4.9
6-deoxy-D-galactose
-
-
0.078 - 3.278
alpha-D-galactose
0.22 - 4.8
D-galactosamine
0.091 - 37.23
D-galactose
15.76
N-acetyl-D-galactosamine
-
pH 8.0, 45°C
0.3
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W
0.97
2-deoxy-D-galactose
pH 7.5, 37°C, wild-type enzyme
0.97
2-deoxy-D-galactose
-
pH 7.5, 37°C, wild-type enzyme
1.1
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme L231S
1.8
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme E244S
1.9
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme E245S
2
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
2.2
2-deoxy-D-galactose
-
pH 7.5, 25°C
2.2
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme Y379W
2.7
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme Q242S
3.6
2-deoxy-D-galactose
-
-
7.4
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme M185L
29.21
2-deoxy-D-galactose
-
wild type enzyme, at 30°C
0.078
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S
0.09
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme G373S
0.099
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M180V
0.15
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S
0.17
alpha-D-galactose
pH 7.5, 37°C, wild-type enzyme
0.17
alpha-D-galactose
pH 7.5, 37°C, wild type enzyme
0.19
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V
0.22
alpha-D-galactose
mutant enzyme E174D, pH 7.5, 37°C
0.22
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V
0.23
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S
0.26
alpha-D-galactose
mutant enzyme R105M, pH 7.5, 37°C
0.26
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme L231S
0.27
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S
0.28
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme A334S
0.31
alpha-D-galactose
mutant enzyme R228K, pH 7.5, 37°C
0.31
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
0.33
alpha-D-galactose
wild-type enzyme, pH 7.5, 37°C
0.44
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme Q242S
0.48
alpha-D-galactose
-
wild-type, pH 8.0, 24°C
0.48
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/A334S
0.53
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme R366Q
0.58
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme D268E
0.627
alpha-D-galactose
pH 7.4, 37°C
0.67
alpha-D-galactose
mutant enzyme R228M, pH 7.5, 37°C
0.698
alpha-D-galactose
-
wild-type protein
0.784
alpha-D-galactose
-
R37A mutant protein
0.823
alpha-D-galactose
-
Y109F mutant protein
0.986
alpha-D-galactose
-
Y236F mutant protein
1
alpha-D-galactose
-
mutant R36K, pH 8.0, 24°C
1
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme E244S
1.1
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme E245S
1.118
alpha-D-galactose
-
Y109H mutant protein
1.12
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M185L/Y379W
1.19
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M185L
1.3
alpha-D-galactose
pH 9.0, 50°C
1.32
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme Y379W
1.8
alpha-D-galactose
-
mutant R36A, pH 8.0, 24°C
3.278
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W
0.0024
ATP
pH 7.5, 37°C, mutant enzyme L231S
0.0027
ATP
pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: D-galactosamine
0.0037
ATP
pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: D-galactosamine
0.0039
ATP
pH 7.5, 37°C, wild-type enzyme
0.0039
ATP
-
mutant E43G, pH 8.0, 37°C
0.0039
ATP
mutant enzyme E174D, pH 7.5, 37°C
0.004
ATP
wild-type enzyme, pH 7.5, 37°C
0.004
ATP
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose
0.0044
ATP
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine
0.005
ATP
-
pH 8, 37°C, mutant enzyme G346S
0.0066
ATP
pH 7.5, 37°C, mutant enzyme E244S
0.0066
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine
0.0077
ATP
pH 7.5, 37°C, mutant enzyme E245S
0.0094
ATP
mutant enzyme R228M, pH 7.5, 37°C
0.0094
ATP
pH 7.5, 37°C, mutant enzyme Q242S
0.0128
ATP
pH 7.5, 37°C, mutant enzyme Q242S
0.0132
ATP
pH 7.5, 37°C, mutant enzyme E245S
0.0134
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-talose
0.0148
ATP
pH 7.5, 37°C, mutant enzyme L231S
0.0149
ATP
mutant enzyme R228K, pH 7.5, 37°C
0.016
ATP
pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: 2-deoxy-D-galactose
0.0162
ATP
pH 7.5, 37°C, wild-type enzyme
0.0173
ATP
pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: 2-deoxy-D-galactose
0.0174
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose
0.018
ATP
-
mutant R36K, pH 8.0, 24°C
0.0198
ATP
pH 7.5, 37°C, mutant enzyme E244S
0.026
ATP
-
pH 8, 37°C, mutant enzyme A198V
0.034
ATP
-
pH 8, 37°C, wild-type enzyme
0.034
ATP
-
wild-type, pH 8.0, 37°C
0.035
ATP
-
mutant E43A, pH 8.0, 37°C
0.0367
ATP
pH 7.5, 37°C, mutant enzyme E244S
0.039
ATP
-
pH 8, 37°C, mutant enzyme G349S
0.0392
ATP
pH 7.5, 37°C, mutant enzyme E245S
0.0432
ATP
pH 7.5, 37°C, wild-type enzyme
0.0432
ATP
pH 7.5, 37°C, wild-type enzyme, cosubstrate: 2-deoxy-D-galactose
0.0446
ATP
pH 7.5, 37°C, mutant enzyme Q242S
0.046
ATP
-
in the presence of 2-deoxy-D-galactose as a substrate, at 30°C, wild type enzyme
0.053
ATP
-
wild-type, pH 8.0, 24°C
0.057
ATP
-
in the presence of D-galactose as a substrate, wild type enzyme
0.0594
ATP
pH 7.5, 37°C, mutant enzyme E244S
0.0594
ATP
pH 7.5, 37°C, mutant enzyme E245S
0.083
ATP
mutant enzyme R105M, pH 7.5, 37°C
0.089
ATP
-
pH 8, 37°C, mutant enzyme G347S
0.0948
ATP
-
wild-type protein
0.1
ATP
pH 8.6, temperature not specified in the publication
0.11
ATP
-
pH 8, 37°C, mutant enzyme R68C
0.13
ATP
-
pH 8, 37°C, mutant enzyme H44Y
0.132
ATP
-
in 50 mM HEPES-NaOH pH 7.5, at 50°C
0.136
ATP
-
Y236F mutant protein
0.14
ATP
-
mutant R36A, pH 8.0, 24°C
0.152
ATP
-
R37A mutant protein
0.162
ATP
pH 7.5, 37°C, wild-type enzyme
0.162
ATP
pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-galactosamine
0.17
ATP
-
ATP in form of MgATP2-
0.17
ATP
pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-talose
0.225
ATP
-
in the presence of D-glucose as a substrate, at 30°C, wild type enzyme
0.271
ATP
-
mutant enzyme D62L, at 30°C
0.296
ATP
-
mutant enzyme D62F, at 30°C
0.33
ATP
-
mutant enzyme A198V
0.34
ATP
-
mutant enzyme D62A, at 30°C
0.36
ATP
pH 8.6, temperature not specified in the publication
0.371
ATP
-
mutant enzyme D62H, at 30°C
0.57
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S, cosubstrate: alpha-D-galactose
0.755
ATP
-
Y109F mutant protein
0.82
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S, cosubstrate: alpha-D-galactose
1
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V, cosubstrate: alpha-D-galactose
2.5
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S, cosubstrate: alpha-D-galactose
3.8
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S, cosubstrate: alpha-D-galactose
3.83
ATP
-
Y109H mutant protein
3.9
ATP
pH 7.5, 37°C, wild type enzyme, cosubstrate: alpha-D-galactose
4.2
ATP
pH 7.5, 37°C, mutant enzyme M60V/A334S, cosubstrate: alpha-D-galactose
5
ATP
pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: alpha-D-galactose
5.4
ATP
pH 7.5, 37°C, mutant enzyme M180V, cosubstrate: alpha-D-galactose
7.7
ATP
pH 7.5, 37°C, mutant enzyme R366Q, cosubstrate: alpha-D-galactose
7.9
ATP
pH 7.5, 37°C, mutant enzyme A334S, cosubstrate: alpha-D-galactose
8.1
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: alpha-D-galactose
11.4
ATP
pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: alpha-D-galactose
12.4
ATP
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: alpha-D-galactose
13
ATP
pH 7.5, 37°C, mutant enzyme D268E, cosubstrate: alpha-D-galactose
13
ATP
pH 7.5, 37°C, mutant enzyme G373S, cosubstrate: alpha-D-galactose
18
ATP
pH 7.5, 37°C, mutant enzyme M60V, cosubstrate: alpha-D-galactose
34.5
ATP
pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: alpha-D-galactose
0.22
D-galactosamine
pH 7.5, 37°C, mutant enzyme Y379W
0.27
D-galactosamine
pH 7.5, 37°C, mutant enzyme M185L/Y379W
0.62
D-galactosamine
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W
0.71
D-galactosamine
pH 7.5, 37°C, wild-type enzyme
0.71
D-galactosamine
-
pH 7.5, 37°C, wild-type enzyme
0.92
D-galactosamine
pH 7.5, 37°C, mutant enzyme E244S
1.2
D-galactosamine
-
pH 7.3, 25°C
2.9
D-galactosamine
pH 7.5, 37°C, mutant enzyme E245S
4.8
D-galactosamine
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
0.091
D-galactose
pH 8.6, temperature not specified in the publication
0.1 - 0.15
D-galactose
-
-
0.108
D-galactose
pH 8.6, temperature not specified in the publication
0.117
D-galactose
-
mutant enzyme A198V
0.14
D-galactose
-
mutant E43G, pH 8.0, 37°C
0.403
D-galactose
-
in 50 mM HEPES-NaOH pH 7.5, at 50°C
0.43
D-galactose
-
pH 8, 37°C, mutant enzyme R68C
0.5
D-galactose
-
pH 7.3, 25°C
0.539
D-galactose
-
wild type enzyme, at 30°C
0.58
D-galactose
-
pH 7.8
0.6
D-galactose
-
pH 7.5, 37°C
0.6
D-galactose
-
pH 8.0, 30°C
0.66
D-galactose
-
pH 8, 37°C, mutant enzyme A198V
0.895
D-galactose
-
pH 8, 37°C
0.97
D-galactose
-
pH 8, 37°C, wild-type enzyme
0.97
D-galactose
-
wild-type, pH 8.0, 37°C
1.1
D-galactose
-
pH 8, 37°C, mutant enzyme G346S
1.11
D-galactose
-
pH 7.5, 25°C
1.7
D-galactose
-
pH 8, 37°C, mutant enzyme G349S
1.85
D-galactose
-
pH 8.7, 25°C
1.9
D-galactose
-
mutant E43A, pH 8.0, 37°C
2.09
D-galactose
-
wild-type, 37°C
4.46
D-galactose
-
mutant Y223F
4.59
D-galactose
-
mutant M173L/Y371H, 37°C
5.2
D-galactose
-
wild-type
5.62
D-galactose
-
mutant Y371H, 37°C
5.63
D-galactose
-
mutant Y223W
5.87
D-galactose
-
mutant M173L, 37°C
7.7
D-galactose
-
pH 8, 37°C, mutant enzyme H44Y
13
D-galactose
-
pH 8, 37°C, mutant enzyme G347S
37.23
D-galactose
-
pH 8.0, 45°C
0.038
D-glucose
-
mutant enzyme D62F, at 30°C
0.107
D-glucose
-
mutant enzyme D62A, at 30°C
0.137
D-glucose
-
mutant enzyme D62L, at 30°C
0.148
D-glucose
-
mutant enzyme D62H, at 30°C
0.225
D-glucose
-
wild type enzyme, at 30°C
2.57
D-glucose
-
mutant M173L, 37°C
4.02
D-glucose
-
mutant M173L/Y371H, 37°C
49.37
D-glucose
-
pH 8.0, 45°C
0.37
D-talose
pH 7.5, 37°C, wild-type enzyme
0.71
D-talose
pH 7.5, 37°C, wild-type enzyme
0.83
D-talose
pH 7.5, 37°C, mutant enzyme E244S
0.92
D-talose
pH 7.5, 37°C, mutant enzyme E245S
2.2
D-talose
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
2.3
D-talose
pH 7.5, 37°C, mutant enzyme Q242S
6.04
L-altrose
-
mutant Y371H, 37°C
6.28
L-altrose
-
mutant M173L/Y371H, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.195
2-amino-deoxy-D-galactose
-
-
0.4 - 15
2-deoxy-D-galactose
0.085
3-deoxy-3-methyl-D-galactose
-
-
0.0483
6-deoxy-D-galactose
-
-
0.05 - 12.9
alpha-D-galactose
0.36
N-acetyl-D-galactosamine
-
pH 8.0, 45°C
0.4
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme L231S
0.4
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme M185L
0.5
2-deoxy-D-galactose
-
-
0.7
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W
0.8
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme Y379W
3.13
2-deoxy-D-galactose
-
wild type enzyme, at 30°C
4.01
2-deoxy-D-galactose
-
wild type enzyme, at 30°C
6.8
2-deoxy-D-galactose
pH 7.5, 37°C, wild-type enzyme
6.8
2-deoxy-D-galactose
-
pH 7.5, 37°C, wild-type enzyme
6.8
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
8
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme E244S
11
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme E245S
15
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme Q242S
0.05
alpha-D-galactose
-
R37A mutant protein
0.1
alpha-D-galactose
-
Y109F mutant protein
0.1
alpha-D-galactose
-
Y109H mutant protein
0.12
alpha-D-galactose
-
Y236F mutant protein
0.15
alpha-D-galactose
-
mutant R36A, pH 8.0, 24°C
0.45
alpha-D-galactose
-
mutant R36K, pH 8.0, 24°C
0.65
alpha-D-galactose
-
wild-type protein
0.7
alpha-D-galactose
mutant enzyme R105M, pH 7.5, 37°C
1
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme L231S
1
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M180V
1.35
alpha-D-galactose
pH 7.4, 37°C
1.5
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V
1.6
alpha-D-galactose
mutant enzyme E174D, pH 7.5, 37°C
1.6
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S
1.7
alpha-D-galactose
wild-type enzyme, pH 7.5, 37°C
1.7
alpha-D-galactose
pH 7.5, 37°C, wild-type enzyme
1.7
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S
1.7
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S
1.7
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S
1.7
alpha-D-galactose
pH 7.5, 37°C, wild type enzyme
2 - 3.7
alpha-D-galactose
pH 9.0, 50°C
2.1
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme R366Q
2.3
alpha-D-galactose
mutant enzyme R228M, pH 7.5, 37°C
2.4
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme G373S
2.5
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M185L/Y379W
2.6
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/A334S
3.2
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme D268E
3.7
alpha-D-galactose
mutant enzyme R228K, pH 7.5, 37°C
4
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme E245S
4.3
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme A334S
4.8
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V
5
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
5.9
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme Q242S
6.1
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme E244S
6.8
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme Y379W
8.1
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M185L
11
alpha-D-galactose
-
wild-type, pH 8.0, 24°C
12.9
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W
0.2
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-talose
0.2
ATP
pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: D-galactosamine
0.3
ATP
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine
0.4
ATP
-
pH 8, 37°C, mutant enzyme G346S
0.4
ATP
pH 7.5, 37°C, mutant enzyme L231S
0.5
ATP
pH 7.5, 37°C, mutant enzyme E244S
0.7
ATP
mutant enzyme R105M, pH 7.5, 37°C
0.7
ATP
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose
0.8
ATP
pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: 2-deoxy-D-galactose
0.91
ATP
-
in 50 mM HEPES-NaOH pH 7.5, at 50°C
1
ATP
pH 7.5, 37°C, mutant enzyme L231S
1
ATP
pH 7.5, 37°C, mutant enzyme M180V, cosubstrate: alpha-D-galactose
1.1
ATP
-
pH 8, 37°C, mutant enzyme G347S
1.5
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V, cosubstrate: alpha-D-galactose
1.6
ATP
mutant enzyme E174D, pH 7.5, 37°C
1.6
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S, cosubstrate: alpha-D-galactose
1.7
ATP
wild-type enzyme, pH 7.5, 37°C
1.7
ATP
pH 7.5, 37°C, wild-type enzyme
1.7
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S, cosubstrate: alpha-D-galactose
1.7
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S, cosubstrate: alpha-D-galactose
1.7
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S, cosubstrate: alpha-D-galactose
1.7
ATP
pH 7.5, 37°C, wild type enzyme, cosubstrate: alpha-D-galactose
1.7
ATP
pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-galactosamine
1.8
ATP
-
pH 8, 37°C, mutant enzyme G349S
2
ATP
-
pH 8, 37°C, mutant enzyme H44Y
2
ATP
pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-talose
2.1
ATP
pH 7.5, 37°C, wild-type enzyme
2.1
ATP
pH 7.5, 37°C, mutant enzyme R366Q, cosubstrate: alpha-D-galactose
2.3
ATP
mutant enzyme R228M, pH 7.5, 37°C
2.4
ATP
pH 7.5, 37°C, mutant enzyme G373S, cosubstrate: alpha-D-galactose
2.5
ATP
pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: alpha-D-galactose
2.6
ATP
pH 7.5, 37°C, mutant enzyme M60V/A334S, cosubstrate: alpha-D-galactose
3.2
ATP
pH 7.5, 37°C, mutant enzyme D268E, cosubstrate: alpha-D-galactose
3.4
ATP
pH 7.5, 37°C, mutant enzyme E245S
3.4
ATP
pH 7.5, 37°C, mutant enzyme Q242S
3.7
ATP
mutant enzyme R228K, pH 7.5, 37°C
3.9
ATP
-
pH 8, 37°C, mutant enzyme R68C
4
ATP
pH 7.5, 37°C, mutant enzyme E245S
4
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine
4.3
ATP
pH 7.5, 37°C, mutant enzyme A334S, cosubstrate: alpha-D-galactose
4.8
ATP
pH 7.5, 37°C, mutant enzyme M60V, cosubstrate: alpha-D-galactose
5
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: alpha-D-galactose
5.9
ATP
-
pH 8, 37°C, mutant enzyme R198V
5.9
ATP
pH 7.5, 37°C, mutant enzyme Q242S
6.1
ATP
pH 7.5, 37°C, mutant enzyme E244S
6.8
ATP
pH 7.5, 37°C, wild-type enzyme
6.8
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose
6.8
ATP
pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: alpha-D-galactose
6.8
ATP
pH 7.5, 37°C, wild-type enzyme, cosubstrate: 2-deoxy-D-galactose
8
ATP
pH 7.5, 37°C, mutant enzyme E244S
8.1
ATP
pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: alpha-D-galactose
8.7
ATP
-
pH 8, 37°C, wild-type enzyme
9.4
ATP
pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: 2-deoxy-D-galactose
11
ATP
pH 7.5, 37°C, mutant enzyme E245S
12.9
ATP
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: alpha-D-galactose
15
ATP
pH 7.5, 37°C, mutant enzyme Q242S
18.1
ATP
pH 7.5, 37°C, mutant enzyme E244S
18.1
ATP
pH 7.5, 37°C, mutant enzyme E245S
25
ATP
pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: D-galactosamine
0.2
D-galactosamine
pH 7.5, 37°C, mutant enzyme Y379W
0.3
D-galactosamine
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W
1.7
D-galactosamine
pH 7.5, 37°C, wild-type enzyme
2.1
D-galactosamine
pH 7.5, 37°C, wild-type enzyme
3.4
D-galactosamine
pH 7.5, 37°C, mutant enzyme E245S
4
D-galactosamine
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
18.1
D-galactosamine
pH 7.5, 37°C, mutant enzyme E244S
25
D-galactosamine
pH 7.5, 37°C, mutant enzyme M185L/Y379W
0.38
D-galactose
pH 8.6, temperature not specified in the publication
0.4
D-galactose
-
pH 8, 37°C, mutant enzyme G346S
0.9
D-galactose
-
mutant E43G, pH 8.0, 37°C
0.91
D-galactose
-
in 50 mM HEPES-NaOH pH 7.5, at 50°C
1.1
D-galactose
-
pH 8, 37°C, mutant enzyme G347S
1.8
D-galactose
-
pH 8, 37°C, mutant enzyme G349S
2
D-galactose
-
pH 8, 37°C, mutant enzyme H44Y
2.61
D-galactose
-
pH 8.0, 45°C
3.9
D-galactose
-
pH 8, 37°C, mutant enzyme R68C
5.9
D-galactose
-
pH 8, 37°C, mutant enzyme R198V
6.7
D-galactose
-
mutant E43A, pH 8.0, 37°C
8.7
D-galactose
-
pH 8, 37°C, wild-type enzyme
8.7
D-galactose
-
wild-type, pH 8.0, 37°C
22.3
D-galactose
-
wild type enzyme, at 30°C
55.8
D-galactose
-
pH 8.0, 30°C
146
D-galactose
-
pH 8, 37°C
0.007
D-glucose
-
mutant enzyme D62F, at 30°C
0.019
D-glucose
-
mutant enzyme D62A, at 30°C
0.034
D-glucose
-
wild type enzyme, at 30°C
0.037
D-glucose
-
mutant enzyme D62L, at 30°C
0.067
D-glucose
-
mutant enzyme D62H, at 30°C
1.1
D-glucose
-
pH 8.0, 45°C
0.2
D-talose
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
0.5
D-talose
pH 7.5, 37°C, mutant enzyme E244S
1.7
D-talose
pH 7.5, 37°C, wild-type enzyme
2
D-talose
pH 7.5, 37°C, wild-type enzyme
3.4
D-talose
pH 7.5, 37°C, mutant enzyme Q242S
18.1
D-talose
pH 7.5, 37°C, mutant enzyme E245S
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0022 - 0.059
2-amino-2-deoxy-D-galactose
0.006 - 7.1
2-deoxy-D-galactose
0.024 - 0.054
3-Deoxy-D-galactose
0.064 - 25
alpha-D-galactose
0.013 - 91
D-galactosamine
0.023
D-glucose
-
pH 8.0, 45°C
0.022
N-acetyl-D-galactosamine
-
pH 8.0, 45°C
0.0022
2-amino-2-deoxy-D-galactose
-
R37A mutant protein
0.0027
2-amino-2-deoxy-D-galactose
-
Y236F mutant protein
0.026
2-amino-2-deoxy-D-galactose
-
Y109F mutant protein
0.032
2-amino-2-deoxy-D-galactose
-
wild-type protein
0.059
2-amino-2-deoxy-D-galactose
-
Y109H mutant protein
0.006
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme M185L
0.019
2-deoxy-D-galactose
-
R37A mutant protein
0.026
2-deoxy-D-galactose
-
Y109H mutant protein
0.037
2-deoxy-D-galactose
-
Y109F mutant protein
0.047
2-deoxy-D-galactose
-
Y236F mutant protein
0.237
2-deoxy-D-galactose
-
wild-type protein
0.39
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme Y379W
0.5
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme L231S
2.3
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W
3.1
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
4.5
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme E244S
5.6
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme Q242S
5.8
2-deoxy-D-galactose
pH 7.5, 37°C, mutant enzyme E245S
7.1
2-deoxy-D-galactose
pH 7.5, 37°C, wild-type enzyme
7.1
2-deoxy-D-galactose
-
pH 7.5, 37°C, wild-type enzyme
0.024
3-Deoxy-D-galactose
-
Y109F mutant protein
0.028
3-Deoxy-D-galactose
-
Y109H mutant protein
0.037
3-Deoxy-D-galactose
-
R37A mutant protein
0.041
3-Deoxy-D-galactose
-
Y236F mutant protein
0.054
3-Deoxy-D-galactose
-
wild-type protein
0.064
alpha-D-galactose
-
R37A mutant protein
0.081
alpha-D-galactose
-
mutant R36A, pH 8.0, 24°C
0.089
alpha-D-galactose
-
Y109H mutant protein
0.12
alpha-D-galactose
-
Y109F mutant protein
0.122
alpha-D-galactose
-
Y236F mutant protein
0.45
alpha-D-galactose
-
mutant R36K, pH 8.0, 24°C
0.93
alpha-D-galactose
-
wild-type protein
2.15
alpha-D-galactose
pH 7.4, 37°C
2.4
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M185L/Y379W
2.7
alpha-D-galactose
mutant enzyme R105M, pH 7.5, 37°C
3.3
alpha-D-galactose
mutant enzyme R228M, pH 7.5, 37°C
3.9
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme E245S
3.9
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme L231S
3.9
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W
4
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme R366Q
5.2
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme Y379W
5.4
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/A334S
6
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme D268E
6.2
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme E244S
6.3
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S
7.3
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S
7.6
alpha-D-galactose
mutant enzyme E174D, pH 7.5, 37°C
8
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M185L
8.8
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V
10.1
alpha-D-galactose
pH 7.5, 37°C, wild-type enzyme
10.1
alpha-D-galactose
wild-type enzyme, pH 7.5, 37°C
10.1
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M180V
10.1
alpha-D-galactose
pH 7.5, 37°C, wild type enzyme
10.9
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S
13.5
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme Q242S
15.4
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme A334S
16.1
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
18.1
alpha-D-galactose
pH 9.0, 50°C
19.6
alpha-D-galactose
mutant enzyme R228K, pH 7.5, 37°C
20
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S
21.8
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme M60V
23
alpha-D-galactose
-
wild-type, pH 8.0, 24°C
25
alpha-D-galactose
pH 7.5, 37°C, mutant enzyme G373S
1
ATP
-
mutant R36A, pH 8.0, 24°C
17.6
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-talose
19.4
ATP
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose
20
ATP
pH 7.5, 37°C, mutant enzyme E245S
23
ATP
pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: 2-deoxy-D-galactose
25
ATP
-
mutant R36K, pH 8.0, 24°C
30
ATP
pH 7.5, 37°C, mutant enzyme L231S
50
ATP
pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: 2-deoxy-D-galactose
50
ATP
pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: D-galactosamine
67
ATP
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine
73
ATP
pH 7.5, 37°C, mutant enzyme E244S
98
ATP
mutant enzyme R105M, pH 7.5, 37°C
110
ATP
pH 7.5, 37°C, wild-type enzyme
110
ATP
pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-galactosamine
110
ATP
pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-talose
185
ATP
pH 7.5, 37°C, mutant enzyme M180V, cosubstrate: alpha-D-galactose
190
ATP
pH 7.5, 37°C, wild-type enzyme
190
ATP
pH 7.5, 37°C, mutant enzyme G373S, cosubstrate: alpha-D-galactose
190
ATP
pH 7.5, 37°C, wild-type enzyme, cosubstrate: 2-deoxy-D-galactose
207
ATP
-
wild-type, pH 8.0, 24°C
210
ATP
pH 7.5, 37°C, mutant enzyme E244S
220
ATP
pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: alpha-D-galactose
236
ATP
pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: alpha-D-galactose
240
ATP
mutant enzyme R228M, pH 7.5, 37°C
240
ATP
pH 7.5, 37°C, mutant enzyme D268E, cosubstrate: alpha-D-galactose
250
ATP
mutant enzyme R228K, pH 7.5, 37°C
270
ATP
pH 7.5, 37°C, mutant enzyme M60V, cosubstrate: alpha-D-galactose
270
ATP
pH 7.5, 37°C, mutant enzyme R366Q, cosubstrate: alpha-D-galactose
300
ATP
pH 7.5, 37°C, mutant enzyme E245S
310
ATP
pH 7.5, 37°C, mutant enzyme E244S
320
ATP
pH 7.5, 37°C, mutant enzyme E245S
340
ATP
mutant enzyme E174D, pH 7.5, 37°C
360
ATP
pH 7.5, 37°C, mutant enzyme E244S
360
ATP
pH 7.5, 37°C, mutant enzyme L231S
380
ATP
pH 7.5, 37°C, mutant enzyme Q242S
390
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S, cosubstrate: alpha-D-galactose
390
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose
420
ATP
pH 7.5, 37°C, mutant enzyme Q242S
430
ATP
pH 7.5, 37°C, wild-type enzyme
430
ATP
wild-type enzyme, pH 7.5, 37°C
430
ATP
pH 7.5, 37°C, wild type enzyme, cosubstrate: alpha-D-galactose
440
ATP
pH 7.5, 37°C, mutant enzyme E245S
550
ATP
pH 7.5, 37°C, mutant enzyme A334S, cosubstrate: alpha-D-galactose
550
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: alpha-D-galactose
600
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine
610
ATP
pH 7.5, 37°C, mutant enzyme Q242S
620
ATP
pH 7.5, 37°C, mutant enzyme M60V/A334S, cosubstrate: alpha-D-galactose
680
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S, cosubstrate: alpha-D-galactose
950
ATP
pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: D-galactosamine
1000
ATP
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W
1360
ATP
pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: alpha-D-galactose
1430
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V, cosubstrate: alpha-D-galactose
2000
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S, cosubstrate: alpha-D-galactose
3000
ATP
pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S, cosubstrate: alpha-D-galactose
0.00057
D-fucose
-
Y236F mutant protein
0.00062
D-fucose
-
R37A mutant protein
0.0047
D-fucose
-
wild-type protein
0.012
D-fucose
-
Y109F mutant protein
0.026
D-fucose
-
Y109H mutant protein
0.013
D-galactosamine
-
Y236F mutant protein
0.047
D-galactosamine
-
Y109H mutant protein
0.06
D-galactosamine
-
Y109F mutant protein
0.066
D-galactosamine
-
R37A mutant protein
0.3
D-galactosamine
pH 7.5, 37°C, mutant enzyme Y379W
0.4
D-galactosamine
pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W
0.615
D-galactosamine
-
wild-type protein
0.9
D-galactosamine
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
1.2
D-galactosamine
pH 7.5, 37°C, mutant enzyme E245S
2.9
D-galactosamine
pH 7.5, 37°C, wild-type enzyme
2.9
D-galactosamine
-
pH 7.5, 37°C, wild-type enzyme
19.7
D-galactosamine
pH 7.5, 37°C, mutant enzyme E244S
91
D-galactosamine
pH 7.5, 37°C, mutant enzyme M185L/Y379W
0.07
D-galactose
-
pH 8.0, 45°C
3.5
D-galactose
pH 8.6, temperature not specified in the publication
164
D-galactose
-
pH 8, 37°C
0.0052
D-talose
-
R37A mutant protein
0.019
D-talose
-
Y109H mutant protein
0.031
D-talose
-
Y109F mutant protein
0.057
D-talose
-
Y236F mutant protein
0.14
D-talose
-
wild-type protein
0.2
D-talose
pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
0.4
D-talose
pH 7.5, 37°C, mutant enzyme E244S
0.4
D-talose
pH 7.5, 37°C, mutant enzyme E245S
1.5
D-talose
pH 7.5, 37°C, mutant enzyme Q242S
2.6
D-talose
pH 7.5, 37°C, wild-type enzyme
2.9
D-talose
pH 7.5, 37°C, wild-type enzyme
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M173L
-
moderate activity on D-glucose, wider substrate specificity than wild-type
M173L/Y371H
-
acts on additional substrates such as 4-azido-4-deoxy-D-galactose, 6-ido-6-deoxy-D-galactose, 6-chloro-6-deoxy-D-galactose, 6-bromo-6-deoxy-D-galactose, 4-deoxy-D-galactose, 6-thio-6-deoxy-D-galactose, 6-thio-6-deoxy-D-glucose, 6-azido-6-deoxy-D-glucose
Y223F
-
activity similar to wild-type, converts additional substrates L-glucose, D-talose, 4-deoxy-D-galactose, less active on D-galactosamine than wild-type
Y223H
-
significant reduction in activity
Y223L
-
significant reduction in activity
Y223R
-
significant reduction in activity
Y223W
-
significant reduction in activity, converts additional substrates L-glucose, D-talose, 4-deoxy-D-galactose, with less efficiency than mutant Y223F, less active on D-galactosamine than wild-type
Y371H
-
wider substrate specificity than wild-type
A384P
-
mutant enzyme is not present in the soluble fraction after sonication and can not be purified
C32M
-
mutant enzyme is not present in the soluble fraction after sonication and can not be purified
D46A
-
no detectable activity
E174Q
no detectable activity, less stable enzyme
E244S
significantly increased (5fold) Michaelis constant for ATP, indicating that this variation impacts on the interaction of the protein with ATP. Significantly increased Michaelis constants for galactose. Turnover number is significantly increased
E245S
catalyzes less efficiently the phosphorylation of galactose than the wild-type enzyme. Significantly increased Michaelis constants for galactose. Turnover number is significantly increased
E43A
-
minor changes in kinetic parameters
E43G
-
10fold drop in turnover number
G346S
-
mutant enzyme shows substantial reduction in turnover number. Lower specificity constant for galactose than wild-type enzyme
G347S
-
mutant enzyme shows substantial reduction in turnover number, increase in Km-value for galactose. Lower specificity constant for galactose than wild-type enzyme
G36R
-
mutant enzyme is not present in the soluble fraction after sonication and can not be purified
H44Y
-
increrase in Km-value for galactose compared to wild-type. Lower specificity constant for galactose than wild-type enzyme
L139P
a loss of stability and function is predicted by molecular docking and simulation modeling
L231S
catalyzes less efficiently the phosphorylation of galactose than the wild-type enzyme. Reduced turnover and decreased catalytic efficiency with the substrates 2-deoxy-D-galactose and ATP
M185L
statistically significantly increased catalytic turnover, with corresponding increases in the Michaelis constants for both substrates ATP and alpha-D-galactose. The specificity constants for galactose and ATP are not significantly changed. This variant is significantly less thermally stable than the wild-type protein and is less resistant to denaturation by urea
M60V/M180V
modest, but significant, increase in the melting temperature compared to the wild type, but no change in the turnover number
M60V/M180V/A334S
none of the steady state kinetic parameters is significantly altered. The variant has significantly increased thermal stability
M60V/M180V/A334S/D268E/G373S
increase in thermal stability, but no significant change in the kinetic parameters
M60V/M180V/A334S/G373S
increase in thermal stability, but no significant change in the kinetic parameters
M60V/M180V/D268E/A334S/R366Q/G373S
mutant enzyme with increased thermal stability and increased turnover towards some substrates. The conformation of the protein is altered at key sites. The number of salt bridges and hydrogen bonds is increased. More stable towards denaturation by urea
Q242S
the turnover number and the catalytic efficiency with ATP (cosubstrate 2-deoxy-D-galactose) are increased. Inactive with D-galactosamine. Turnover number is significantly increased
R105M
modest reduction in activity with little change in stability
R228M
interaction with both ATP and galactose is affected. The variant is significantly less stable than the wild-type protein
R256W
-
drastic reduction of activity when expressed in COS cells, missense mutation causes GALK deficiency
T288M
-
mutant enzyme is not present in the soluble fraction after sonication and can not be purified
T344M
-
drastic reduction of activity when expressed in COS cells, missense mutation causes GALK deficiency
Y379W
a variant with greater substrate promiscuity than wild-type enzyme
R36A
-
100fold decrease in activity
R36N
-
100fold decrease in activity
D186A
-
residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme
D186E
-
residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme
D186N
-
residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme
R37A
-
residue R37 interacts with galactose
Y109F
-
residue Y109interacts with ATP
Y109H
-
residue Y109interacts with ATP
Y236A
-
residue Y236 interacts with galactose
Y236F
-
residue Y236 interacts with galactose
D62A
-
abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate
D62A/Y274A
-
shows no sugar kinase activity
D62C
-
shows no sugar kinase activity
D62E
-
shows no detectable sugar kinase activity
D62F
-
abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate
D62G
-
shows no sugar kinase activity
D62H
-
abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate
D62I
-
shows no sugar kinase activity
D62K
-
shows no detectable sugar kinase activity
D62L
-
abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate
D62M
-
shows no sugar kinase activity
D62N
-
shows no detectable sugar kinase activity
D62P
-
shows no sugar kinase activity
D62Q
-
shows no sugar kinase activity
D62R
-
shows no sugar kinase activity
D62S
-
shows no sugar kinase activity
D62T
-
shows no sugar kinase activity
D62V
-
shows no sugar kinase activity
D62W
-
shows no sugar kinase activity
D62Y
-
shows no sugar kinase activity
R53A/D217A
-
shows no sugar kinase activity
D62A
-
abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate
-
D62E
-
shows no detectable sugar kinase activity
-
D62G
-
shows no sugar kinase activity
-
D62M
-
shows no sugar kinase activity
-
D62N
-
shows no detectable sugar kinase activity
-
A198V
-
GALK variant associated with an A198V mutation in three infants with mild GALK deficiency. Km-values from healthy and mutant individuals are similar. The variant A198V probably originates in Japanese and Korean ancestors and is one of the genetic factors that cause cataract in elderly individuals
A198V
-
very similar kinetic parameters to the wild-type enzyme
A198V
a loss of stability and function is predicted by molecular docking and simulation modeling
G349S
-
lower specificity constant for galactose than wild-type enzyme. Lower specificity constant for galactose than wild-type enzyme
G349S
-
drastic reduction of activity when expressed in COS cells, missense mutation causes GALK deficiency
P28T
-
mutant enzyme is not present in the soluble fraction after sonication and can not be purified
P28T
a loss of stability and function is predicted by molecular docking and simulation modeling
additional information
-
all exchanges for D37 are detrimental to the enzyme
additional information
-
the two deletions, of 410delG and 509-510delGT , occur at the nucleotide repeats GGGGGG and GTGTGT, respectively and result in in-frame nonsense codons at amino acids 163 and 201. These mutations arise by slipped strand mispairing
additional information
-
enzyme deletion mutant, strain shows reduced growth on D-galactose but is only slightly affected in growth on lactose. In mutant strain, induction of D-galactose-1-phosphate uridylyltransferase by D-galactose, but not by L-arabinose, is impaired
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