Information on EC 2.7.1.3 - ketohexokinase

Word Map on EC 2.7.1.3
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
2.7.1.3
-
RECOMMENDED NAME
GeneOntology No.
ketohexokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-fructose = ADP + D-fructose 1-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sucrose degradation V (sucrose alpha-glucosidase)
-
-
Fructose and mannose metabolism
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-fructose 1-phosphotransferase
D-Sorbose, D-tagatose and 5-dehydro-D-fructose and a number of other ketoses and their analogues can also act as substrates [4].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-50-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
two mouse knockout models are generated: one strain is selectively deficient in peripheral Khk-A, while the second strain lacks activity of both Khk-A and Khk-C. Both homozygous knockout mice are healthy and fertile and display minimal biochemical abnormalities under basal dietary conditions
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2,5-anhydro-D-glucitol
?
show the reaction diagram
ATP + 2,5-anhydro-D-lyxitol
?
show the reaction diagram
ATP + 2,5-anhydro-D-mannitol
?
show the reaction diagram
ATP + 2,5-anhydro-D-mannose
?
show the reaction diagram
ATP + D-fructose
ADP + D-fructose 1-phosphate
show the reaction diagram
ATP + D-psicose
?
show the reaction diagram
-
-
-
-
r
ATP + D-ribono-gamma-lactone
?
show the reaction diagram
-
-
-
-
r
ATP + D-ribose
?
show the reaction diagram
ATP + D-ribulose
?
show the reaction diagram
-
-
-
-
?
ATP + D-sedoheptulose
?
show the reaction diagram
-
-
-
-
r
ATP + D-sorbose
?
show the reaction diagram
ATP + D-tagatose
?
show the reaction diagram
ATP + D-xylulose
?
show the reaction diagram
ATP + L-galactoheptulose
?
show the reaction diagram
-
-
-
-
r
ATP + L-ribulose
?
show the reaction diagram
-
-
-
-
r
ATP + L-sorbose
?
show the reaction diagram
ATP + mannoheptulose
?
show the reaction diagram
-
-
-
-
r
GTP + D-fructose
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose
ADP + D-fructose 1-phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CsCl
-
the enzyme is maximally active in the presence of 1.2 M KCl. Activity in the presence of RbCl and CsCl is similar to that observed in the presence of KCl
KCl
-
the enzyme is maximally active in the presence of 1.2 M KCl. Activity in the presence of RbCl and CsCl is similar to that observed in the presence of KCl
Li+
-
absolute requirement for both monovalent and divalent cations, when Mg2+ fills the requirement for divalent cation, K+ and Rb+ are the most active monovalent cations, NH4+, Na+, Cs+ and Li+ are decreasingly active
Mn2+
-
inactive in absence of Mg2+ or Mn2+
NaCl
-
stimulates, yields 50% of the activity observed in equimolar KCl
RbCl
-
the enzyme is maximally active in the presence of 1.2 M KCl. Activity in the presence of RbCl and CsCl is similar to that observed in the presence of KCl
Tl+
-
activates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-deoxy-D-fructose
-
dead-end inhibitor
2,5-anhydro-D-glucitol
-
-
2,5-anhydro-D-lyxitol
-
-
2,5-anhydro-D-mannitol
-
-
2,5-anhydro-D-mannose
-
-
2,5-Anhydro-D-xylitol
-
-
2-(4-aminopiperidin-1-yl)-N8-(cyclopropylmethyl)-N4-(2-methylphenyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
2-[(3R)-3-aminopiperidin-1-yl]-N8-(cyclopropylmethyl)-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
2-[(3S)-3-aminopiperidin-1-yl]-N8-(cyclopropylmethyl)-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
2-[3-(aminomethyl)azetidin-1-yl]-N8-(cyclopropylmethyl)-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
2-[4-(aminomethyl)piperidin-1-yl]-N8-(cyclopropylmethyl)-N4-(2-methylphenyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
2-[4-(aminomethyl)piperidin-1-yl]-N8-(cyclopropylmethyl)-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
4-chloromercuribenzoate
-
-
5-Thio-D-fructose
-
competitive to D-fructose
ATP
-
-
CrADP
-
dead-end inhibitor
CrATP
-
dead-end inhibitor
D-Fructose 1-phosphate
-
competitive against D-fructose, noncompetitive to MgATP2-
D-mannoheptulose
-
-
D-psicose
-
-
D-ribono-gamma-lactone
-
-
D-ribose
-
-
D-sedoheptulose
-
-
D-tagatose
-
-
D-xylulose
-
-
L-galactoheptulose
-
-
L-ribulose
-
-
L-sorbose
-
-
MgADP-
-
competitive to MgATP2-
N,N'-dicyclopropyl-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N-ethylmaleimide
-
-
N4-(2-bromophenyl)-N8-cyclopropyl-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N4-(2-chlorophenyl)-N8-cyclopropyl-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N4-(2-methylphenyl)-2-(piperazin-1-yl)-N8-(1,3-thiazol-2-ylmethyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N4-(2-methylphenyl)-2-(piperazin-1-yl)-N8-(prop-2-yn-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N4-(2-methylphenyl)-2-(piperazin-1-yl)-N8-(tetrahydrothiophen-2-ylmethyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N4-(2-methylphenyl)-2-(piperazin-1-yl)-N8-propylpyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N4-cyclohexyl-N8-cyclopropyl-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)-N8-(1,3-thiazol-2-ylmethyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)-N8-(pyridin-2-ylmethyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)-N8-(thiophen-2-ylmethyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-(2-cyclopropylethyl)-N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-(2-methoxyethyl)-N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-(cyclobutylmethyl)-N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-(cyclopropylmethyl)-2-(1,4-diazepan-1-yl)-N4-(2-methylphenyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-(cyclopropylmethyl)-2-(4-iminopiperidin-1-yl)-N4-(2-methylphenyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-(cyclopropylmethyl)-2-(4-methylpiperazin-1-yl)-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-(cyclopropylmethyl)-2-[4-[(dimethylamino)methyl]piperidin-1-yl]-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-(cyclopropylmethyl)-N2-methyl-N2-[2-(methylamino)ethyl]-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-2,4,8-triamine
-
-
N8-(cyclopropylmethyl)-N4-(2-methylphenyl)-2-(4-methylpiperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-(cyclopropylmethyl)-N4-(2-methylphenyl)-2-(morpholin-4-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-benzyl-N4-(2-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclohexyl-N4-(2-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-2-(piperazin-1-yl)-N4-[2-(propan-2-yl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-2-(piperazin-1-yl)-N4-[2-[(trifluoromethyl)sulfanyl]phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-(2-cyclopropylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-(2-ethoxyphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-(2-ethylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-(2-fluorophenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-(2-methoxyphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-(2-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-(3-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-phenyl-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-[2-(ethylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-[2-(methylsulfonyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-[3-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-cyclopropyl-N4-[4-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-ethyl-N4-(2-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-hexyl-N4-(2-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
N8-methyl-N4-(2-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
-
-
PCMB
-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.9 - 47
2,5-anhydro-D-glucitol
10 - 67
2,5-anhydro-D-lyxitol
1.6 - 6.3
2,5-anhydro-D-mannitol
0.31 - 1.5
2,5-anhydro-D-mannose
1.2
5-keto-D-fructose
-
25C, pH 7
1.43 - 3.3
ATP
0.1 - 8.2
D-fructose
120
D-mannoheptulose
-
25C, pH 7
11
D-psicose
-
25C, pH 7
58
D-ribono-gamma-lactone
-
25C, pH 7
142 - 434
D-ribose
1.8 - 32.7
D-ribulose
2.2
D-sedoheptulose
-
25C, pH 7
0.9
D-tagatose
0.44 - 1.4
D-xylose
6
D-xylulose
-
37C, pH 7.5
1.53
GTP
-
pH 7.4
1.8
L-galacto-2-heptulose
-
25C, pH 7, D-fructose 1-phosphate
0.37
L-ribulose
-
25C, pH 7
0.4 - 19.3
L-sorbose
0.15 - 0.41
MgATP2-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.7 - 7.6
D-fructose
1.55 - 8.2
D-ribose
4.8 - 7.6
D-ribulose
7.6 - 11.1
D-xylulose
5.2 - 6.5
L-sorbose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1 - 0.2
ADP
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0002
2-(4-aminopiperidin-1-yl)-N8-(cyclopropylmethyl)-N4-(2-methylphenyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.000018
2-[(3R)-3-aminopiperidin-1-yl]-N8-(cyclopropylmethyl)-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.000023
2-[(3S)-3-aminopiperidin-1-yl]-N8-(cyclopropylmethyl)-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00003
2-[3-(aminomethyl)azetidin-1-yl]-N8-(cyclopropylmethyl)-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00007
2-[4-(aminomethyl)piperidin-1-yl]-N8-(cyclopropylmethyl)-N4-(2-methylphenyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00001
2-[4-(aminomethyl)piperidin-1-yl]-N8-(cyclopropylmethyl)-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.009
N,N'-dicyclopropyl-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00017
N4-(2-bromophenyl)-N8-cyclopropyl-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00054
N4-(2-chlorophenyl)-N8-cyclopropyl-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00006
N4-(2-methylphenyl)-2-(piperazin-1-yl)-N8-(1,3-thiazol-2-ylmethyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0017
N4-(2-methylphenyl)-2-(piperazin-1-yl)-N8-(prop-2-yn-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0003
N4-(2-methylphenyl)-2-(piperazin-1-yl)-N8-(tetrahydrothiophen-2-ylmethyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0004
N4-(2-methylphenyl)-2-(piperazin-1-yl)-N8-propylpyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.009
N4-cyclohexyl-N8-cyclopropyl-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.000016
N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)-N8-(1,3-thiazol-2-ylmethyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0000098
N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)-N8-(pyridin-2-ylmethyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00003
N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)-N8-(thiophen-2-ylmethyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0000071
N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00005
N8-(2-cyclopropylethyl)-N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.000007
N8-(2-methoxyethyl)-N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.000018
N8-(cyclobutylmethyl)-N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0003
N8-(cyclopropylmethyl)-2-(1,4-diazepan-1-yl)-N4-(2-methylphenyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00071
N8-(cyclopropylmethyl)-2-(4-iminopiperidin-1-yl)-N4-(2-methylphenyl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00011
N8-(cyclopropylmethyl)-2-(4-methylpiperazin-1-yl)-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00014
N8-(cyclopropylmethyl)-2-[4-[(dimethylamino)methyl]piperidin-1-yl]-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00013
N8-(cyclopropylmethyl)-N2-methyl-N2-[2-(methylamino)ethyl]-N4-[2-(methylsulfanyl)phenyl]pyrimido[5,4-d]pyrimidine-2,4,8-triamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0015
N8-(cyclopropylmethyl)-N4-(2-methylphenyl)-2-(4-methylpiperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.007
N8-(cyclopropylmethyl)-N4-(2-methylphenyl)-2-(morpholin-4-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0003
N8-benzyl-N4-(2-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0016
N8-cyclohexyl-N4-(2-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.005
N8-cyclopropyl-2-(piperazin-1-yl)-N4-[2-(propan-2-yl)phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.003
N8-cyclopropyl-2-(piperazin-1-yl)-N4-[2-[(trifluoromethyl)sulfanyl]phenyl]pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00038
N8-cyclopropyl-N4-(2-cyclopropylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0002
N8-cyclopropyl-N4-(2-ethoxyphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00013
N8-cyclopropyl-N4-(2-ethylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0015
N8-cyclopropyl-N4-(2-fluorophenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0001
N8-cyclopropyl-N4-(2-methoxyphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.00021
N8-cyclopropyl-N4-(2-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0028
N8-cyclopropyl-N4-(3-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0032
N8-cyclopropyl-N4-phenyl-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.009
N8-cyclopropyl-N4-[2-(ethylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.000012
N8-cyclopropyl-N4-[2-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.009
N8-cyclopropyl-N4-[2-(methylsulfonyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.002
N8-cyclopropyl-N4-[3-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.009
N8-cyclopropyl-N4-[4-(methylsulfanyl)phenyl]-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0023
N8-ethyl-N4-(2-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
Homo sapiens
-
pH and temperature not specified in the publication
0.0004
N8-hexyl-N4-(2-methylphenyl)-2-(piperazin-1-yl)pyrimido[5,4-d]pyrimidine-4,8-diamine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00014
-
in B and non-B cells. The reaction velocity is recorded in the presence of D-fructose (1.0 mM) and K+ in heated homogenate, it is expressed relative to protein content.
0.000158
-
in parotid gland. The reaction velocity is recorded in the presence of D-fructose (1.0 mM) and K+ in heated homogenate, it is expressed relative to protein content.
0.00046
-
in islets. The reaction velocity is recorded in the presence of D-fructose (1.0 mM) and K+ in heated homogenate, it is expressed relative to protein content.
0.00082
-
in pancreas. The reaction velocity is recorded in the presence of D-fructose (1.0 mM) and K+ in heated homogenate, it is expressed relative to protein content.
0.00245
-
in ileum. The reaction velocity is recorded in the presence of D-fructose (1.0 mM) and K+ in heated homogenate, it is expressed relative to protein content.
0.02154
-
in liver. The reaction velocity is recorded in the presence of D-fructose (1.0 mM) and K+ in heated homogenate, it is expressed relative to protein content.
9.4
-
-
additional information
-
enzyme activities in transgenic plants of Solanum tuberosum
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.8
-
-
7
-
assay at
7.4
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.1 - 9.1
-
pH 5.1: about 20% of activity maximum, pH 9.1: about 30% of activity maximum
7.5 - 10.5
-
activity increases about twofold from pH 7.5 to pH 10.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
heat resistant
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
-
2-D gel analysis
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
relatively low expression of ketohexokinase.The expression profile for ketohexokinase in human is derived from from Unigene by adding the number of EST clones obtained from normal tissues
Manually annotated by BRENDA team
-
relatively low expression of ketohexokinase.The expression profile for ketohexokinase in human is derived from from Unigene by adding the number of EST clones obtained from normal tissues
Manually annotated by BRENDA team
-
12.4% of the phosphorylation of D-fructose by ileum homogenates is attributable to ketohexokinase
Manually annotated by BRENDA team
-
relatively high expression of ketohexokinase.The expression profile for ketohexokinase in human is derived from from Unigene by adding the number of EST clones obtained from normal tissues
Manually annotated by BRENDA team
-
relatively low expression of ketohexokinase.The expression profile for ketohexokinase in human is derived from from Unigene by adding the number of EST clones obtained from normal tissues
Manually annotated by BRENDA team
-
represent the most prevalent cancer of the kidney. The mean ketohexokinase activity in the renal cell carcinoma tissue samples is approximately 1.4fold lower than that in their normal kidney tissue. Ketohexokinase activity is approximately 1.5fold lower in pT3 renal cell carcinoma tissue than that in pT1 renal cell carcinoma tissue. The enzyme activty of tumor in histological grade 3 is 1.8fold lower than for grade 1 tumor(reaction mixture activity assay: acid-treated supernatant (100 microl) containing 10 mM MgCl2, 50 mM TrisHCl, pH 7.4, 10 mM ATP, 50 mM N-acetyl-D-glucosamine, 10 mM D[UL-14C]fructose and water, at 37C). The expression level of ketohexokinase mRNA is reduced two- to eightfold in nearly all the clear cell renal cell carcinoma tissue samples compared with normal kidney, except for one renal cell carcinoma sample.
Manually annotated by BRENDA team
-
relatively low expression of ketohexokinase.The expression profile for ketohexokinase in human is derived from from Unigene by adding the number of EST clones obtained from normal tissues
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32730
-
2-D gel analysis; theoretical
56000
-
gel filtration, ultracentrifugation
75000
-
gel filtration
95500
-
gel filtration
100000
-
sucrose density gradient sedimentation analysis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
? * 33000, immunoblot
homodimer
-
gel filtration, nondenaturing PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
at 20C, 10 mg/ml protein pH 7.5 in 10 mM HEPES, vapour-diffusion method applied in hanging- and sitting-drop variants, protein desalted and with Centricon-10 concentrated, precipitants: 2-propanol and MPD, space group: P212121 or P21212 with a: 93.4, b: 121.5 and c: 108.4 A
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
25C, 2 h, totally inactivated in 0.1 M KCl/50 mM Tris-HCl (pH 9.0). Stabilizing effect of KCl increases with increasing KCl concentration between 0.1 and 2.5 M (ranging from 30 to 98% of the total). Addition of 50 mM phosphate aids in stabilization of ketohexokinase activity in 0.1 M KCI for about 24 h. Although it is inhibitory for expression of ketohexokinase activity, (NH4)2SO4 stabilizes
37
-
C-A43T rapidly loss in activity in absence of glycerol
55
-
30 min ketohexokinase-A little loss in activity, ketohexokinase-C completely inactivated
75
-
30 min, 5% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
(NH4)2SO4 stabilizes
-
stabilizing effect of KCl increases with increasing KCl concentration between 0.1 and 2.5 M (ranging from 30 to 98% of the total)
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, stable for several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
construction of transgenic Solanum tuberosum plants expressing the enzyme under control of the CaMV 35S promotor by Agrobacterium tumefaciens infection system
-
expressed in Escherichia coli
-
overexpression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A43T
-
decreased thermostability of ketohexokinase-A and ketohexokinase-C
G40R
-
both ketohexokinase-A and ketohexokinase-C become inactive and largely unsoluble
additional information
-
heterologous expression of the enzyme in potato plants, showing elevated activity in leaves and tubers, leads to inhibited rates of photosynthesis, severe growth retardation and abnormal leaf development, phenotype analysis