Information on EC 2.5.1.51 - beta-pyrazolylalanine synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Citrullus lanatus subsp. vulgaris

EC NUMBER
COMMENTARY hide
2.5.1.51
-
RECOMMENDED NAME
GeneOntology No.
beta-pyrazolylalanine synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
O-acetyl-L-serine + pyrazole = 3-(pyrazol-1-yl)-L-alanine + acetate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aminopropanoyl-group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta-pyrazole-1-ylalanine biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
O3-acetyl-L-serine:pyrazole 1-(2-amino-2-carboxyethyl)transferase
The enzyme is highly specific for acetylserine and pyrazole. Not identical with EC 2.5.1.52 L-mimosine synthase.
CAS REGISTRY NUMBER
COMMENTARY hide
37290-81-6
not distinguished from EC 4.2.1.50 in Chemical Abstracts
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
O-succinyl-L-serine + pyrazole
3-(pyrazol-1-yl)-L-alanine + succinate
show the reaction diagram
-
at 16% the rate of O-acetyl-L-serine
-
-
?
O-sulfo-L-serine + pyrazole
3-(pyrazol-1-yl)-L-alanine + SO42-
show the reaction diagram
-
at 2.5% the rate of O-acetyl-L-serine
-
-
?
O3-acetyl-L-serine + 3-amino-1,2,4-triazole
3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
show the reaction diagram
-
at 11% the rate of pyrazole
-
-
O3-acetyl-L-serine + N-hydroxyurea
O-ureidoserine + acetate
show the reaction diagram
-
at 9% the rate of pyrazole
-
?
O3-acetyl-L-serine + pyrazole
3-(pyrazol-1-yl)-L-alanine + acetate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
O3-acetyl-L-serine + pyrazole
3-(pyrazol-1-yl)-L-alanine + acetate
show the reaction diagram
-
involved in biosynthesis of heterocyclic beta-substituted alanines
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit, further increase of activity by 40% by adding pyridoxal phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxylamine
-
50 mM, almost complete inhibition
KCN
-
10 mM, almost complete inhibition
additional information
-
no inhibition by pyridoxal phosphate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
O-acetylserine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
-
about half-maximal activity at pH 6.5 and 8.5
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
barely detectable in dry seeds
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
-
2 * 32000, SDS-PAGE
58000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 32000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freeze-thawing or lyophilization inactivates
-
sensitive to freezing even in the presence of 20% glycerol
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-2°C, stable for at least 1 month
-
0°C, crude enzyme preparation, 25% loss of activity within 22 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE