General Stability | Organism |
---|---|
freeze-thawing or lyophilization inactivates | Citrullus lanatus subsp. vulgaris |
sensitive to freezing even in the presence of 20% glycerol | Citrullus lanatus subsp. vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | 50 mM, almost complete inhibition | Citrullus lanatus subsp. vulgaris | |
KCN | 10 mM, almost complete inhibition | Citrullus lanatus subsp. vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
O-acetylserine | - |
Citrullus lanatus subsp. vulgaris | |
74 | - |
pyrazole | - |
Citrullus lanatus subsp. vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Citrullus lanatus subsp. vulgaris |
58000 | - |
gel filtration | Citrullus lanatus subsp. vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O3-acetyl-L-serine + pyrazole | Citrullus lanatus subsp. vulgaris | involved in biosynthesis of heterocyclic beta-substituted alanines | 3-(pyrazol-1-yl)-L-alanine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrullus lanatus subsp. vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Citrullus lanatus subsp. vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | barely detectable in dry seeds | Citrullus lanatus subsp. vulgaris | - |
seedling | cotyledons removed, 1 week old | Citrullus lanatus subsp. vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.355 | - |
- |
Citrullus lanatus subsp. vulgaris |
Storage Stability | Organism |
---|---|
0-2°C, stable for at least 1 month | Citrullus lanatus subsp. vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-succinyl-L-serine + pyrazole | at 16% the rate of O-acetyl-L-serine | Citrullus lanatus subsp. vulgaris | 3-(pyrazol-1-yl)-L-alanine + succinate | - |
? | |
O-sulfo-L-serine + pyrazole | at 2.5% the rate of O-acetyl-L-serine | Citrullus lanatus subsp. vulgaris | 3-(pyrazol-1-yl)-L-alanine + SO42- | - |
? | |
O3-acetyl-L-serine + 3-amino-1,2,4-triazole | at 11% the rate of pyrazole | Citrullus lanatus subsp. vulgaris | 3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate | - |
? | |
O3-acetyl-L-serine + N-hydroxyurea | at 9% the rate of pyrazole | Citrullus lanatus subsp. vulgaris | O-ureidoserine + acetate | - |
? | |
O3-acetyl-L-serine + pyrazole | highly specific | Citrullus lanatus subsp. vulgaris | 3-(pyrazol-1-yl)-L-alanine + acetate | - |
? | |
O3-acetyl-L-serine + pyrazole | involved in biosynthesis of heterocyclic beta-substituted alanines | Citrullus lanatus subsp. vulgaris | 3-(pyrazol-1-yl)-L-alanine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Citrullus lanatus subsp. vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 7.4 | - |
Citrullus lanatus subsp. vulgaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | about half-maximal activity at pH 6.5 and 8.5 | Citrullus lanatus subsp. vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit, further increase of activity by 40% by adding pyridoxal phosphate | Citrullus lanatus subsp. vulgaris |